Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion

Abstract

Probiotics are known to contribute to the anti-oxidation, immunoregulation, and aging delay. Here, we investigated the extension of lifespan by fermented pickles-origin Pediococcus acidilactici (PA) in Caenorhabditis elegans (C. elegans), and found that PA promoted a significantly extended longevity of wild-type C. elegans. The further results revealed that PA regulated the longevity via promoting the insulin/IGF-1 signaling, JNK/MAPK signaling but not TOR signaling in C. elegans, and that PA reduced the reactive oxygen species (ROS) levels and modulated expression of genes involved in fatty acids uptake and lipolysis, thus reducing the fat accumulation in C. elegans. Moreover, this study identified the nrfl-1 as the key regulator of the PA-mediated longevity, and the nrfl-1/daf-18 signaling might be activated. Further, we highlighted the roles of one chloride ion exchanger gene sulp-6 in the survival of C. elegans and other two chloride ion channel genes clh-1 and clh-4 in the prolonged lifespan by PA-feeding through the modulating expression of genes involved in inflammation. Therefore, these findings reveal the detailed and novel molecular mechanisms on the longevity of C. elegans promoted by PA.

Saccharomyces cerevisiae: a patulin degradation candidate bothin vitroandin vivo

“…55 Besides, Pediococcus acidilactici also up-regulated the sod-3, fat-4 and ltpl-4 genes in C. elegans to reduce the ROS level and lipid disposition to extend lifespan. 56 These research findings are consistent with the up-regulation of sod-3 in the wild-type KD group in this work. In the daf-2 mutant, due to the defect of the daf-2 gene and patulin exposure, genes daf-16, sod-3 and skn-1 declined in the KD group.…”

Betulinic acid increases lifespan and stress resistance via insulin/IGF-1 signaling pathway in Caenorhabditis elegans

“…For the pharyngeal pumping assay, the synchronized C. elegans were visualized at the magnification of 50X under a stereomicroscope. The pumping frequency of the terminal pharyngeal bulb was counted for 30s at room temperature (25).…”

Approaches to discern if microbiome associations reflect causation in metabolic and immune disorders

“… 31 For C. elegans , several studies have investigated the role of chosen specific bacterial strains (or consortia) – frequently lactic acid bacteria, on lifespan, fat metabolism, or metabolic pathways involved in energy metabolism regulation. 32 , 33 The worm has also been extensively used in the field of host-pathogen interaction, triggering the regulation of innate immunity 34 as well as a cascade of a wide variety of genes. 35 Similarly, Drosophila has been extensively used as a model to study the phenomenology and the underlying molecular and cellular mechanisms of microbiota-diet interactions and their influence on host biology including postnatal development (ie juvenile growth and maturation), adult physiology (ie immune and metabolic functions and behavior) and aging.…”