2012
DOI: 10.1099/ijs.0.046201-0
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Pediococcus lolii DSM 19927T and JCM 15055T are strains of Pediococcus acidilactici

Abstract: Strain NGRI 0510QT , isolated from ryegrass silage, was recently classified as a representative of a novel Pediococcus species, Pediococcus lolii Doi et al. 2009. It was deposited in the DSMZ and JCM culture collections as DSM 19927 T and JCM 15055 T , respectively. A polyphasic taxonomic study, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, pheS and 16S rRNA gene sequence analysis, fluorescent amplified fragment length polymorphism and DNA-DNA hybridization, was used… Show more

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Cited by 42 publications
(40 citation statements)
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“…Isolates were subcultured twice using the respective isolation conditions and MALDI-TOF MS was performed using the third generation of pure cultures by means of a 4800 Plus MALDI TOF/ TOF™ Analyzer (AB SCIEX, Framingham, MA, USA), as described previously (Wieme et al, 2012). In short, Data Explorer 4.0 software (AB SCIEX) was used to convert the mass spectra into txt-files to import them into a BioNumerics 5.1 (Applied Maths) database.…”
Section: Matrix-assisted Laser Desorption/ionization Time-of-flight Mmentioning
confidence: 99%
“…Isolates were subcultured twice using the respective isolation conditions and MALDI-TOF MS was performed using the third generation of pure cultures by means of a 4800 Plus MALDI TOF/ TOF™ Analyzer (AB SCIEX, Framingham, MA, USA), as described previously (Wieme et al, 2012). In short, Data Explorer 4.0 software (AB SCIEX) was used to convert the mass spectra into txt-files to import them into a BioNumerics 5.1 (Applied Maths) database.…”
Section: Matrix-assisted Laser Desorption/ionization Time-of-flight Mmentioning
confidence: 99%
“…All conditions were as previously described except that YMA growth medium was used to culture the strains prior to protein extraction (Wieme et al, 2012). All strains representing these novel species formed distinct clusters that could be distinguished from the strains representing the closest neighbour M. ciceri (Fig.…”
Section: Mesorhizobium Thiogangeticum Sjt T (Aj864462)mentioning
confidence: 99%
“…Bradyrhizobium elkanii USDA 76 T (U35000) Five novel Mesorhizobium species (Wieme et al, 2012). All conditions were as previously described except that YMA growth medium was used to culture the strains prior to protein extraction (Wieme et al, 2012).…”
Section: Mesorhizobium Thiogangeticum Sjt T (Aj864462)mentioning
confidence: 99%
“…Cell extraction for MALDI-TOF MS analysis was performed as described by Wieme et al (2012). Bacterial fingerprints were acquired using the 4800 Plus MALDI TOF/TOF Analyzer (Applied Biosystems) in linear positive ion mode as described previously (Wieme et al, 2012). For each spot, 50 sub-spectra for each of 40 randomized positions within the spot were collected and presented as one main spectrum.…”
mentioning
confidence: 99%
“…Cells were grown on Mueller Hinton agar base (Oxoid) supplemented with 5 % sheep blood for 72 h at 37 C under microaerobic conditions. Cell extraction for MALDI-TOF MS analysis was performed as described by Wieme et al (2012). Bacterial fingerprints were acquired using the 4800 Plus MALDI TOF/TOF Analyzer (Applied Biosystems) in linear positive ion mode as described previously (Wieme et al, 2012).…”
mentioning
confidence: 99%