Peer Review #1 of "Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy (v0.1)"

Abstract

We analyzed the applicability of high-resolution 2 H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondria isolated from rat liver and from aqueous extracts of homogenates of rat liver in the presence of high D 2 O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.