Peer Review #3 of "Clinical characteristics and prognostic value of MEX3A mRNA in liver cancer (v0.2)"

Abstract

Background. MEX3A is an RNA-binding proteins (RBPs) that promotes the proliferation, invasion, migration and viability of cancer cells. The aim of this study was to explore the clinicopathological characteristics and prognostic significance of MEX3A mRNA expression in liver cancer. Methods. RNA-Seq and clinical data were collected from The Cancer Genome Atlas (TCGA). Boxplots were used to represent discrete variables of MEX3A. Chisquare tests were used to analyze the correlation between clinical features and MEX3A expression. Receiver operating characteristic (ROC) curves were used to confirm diagnostic ability. Independent prognostic ability and values were assessed using Kaplan-Meier curves and Cox analysis. Results. We acquired MEX3A RNA-Seq from 50 normal liver tissues and 373 liver cancer patients along with clinical data. We found that MEX3A was up-regulated in liver cancer which increased according to histological grade (p<0.001). MEX3A showed moderate diagnostic ability for liver cancer (AUC=0.837). Kaplan-Meier curves and Cox analysis revealed that the high expression of MEX3A was significantly associated with poor survival (OS and RFS) (p<0.001). Moreover, MEX3A was identified as an independent prognostic factor of liver cancer (p<0.001). Conclusions. MEX3A expression shows promise as an independent predictor of liver cancer prognosis.