Peer Review #4 of "Silk derived formulations for accelerated wound healing in diabetic mice (v0.2)"

Abstract

Background: The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods: Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis.Results: The results indicated that the best biocompatible silk combination was of 5 % silk fibroin and 5 % Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5±0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion: It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.