Peer Reviewed: Genomics Tools in Environmental Impact Assessment

Purohit, Hemant J.; Raje, Dhanajay V.; Kapley, Atya; Padmanabhan, Parsuraman; Singh, R. N.

doi:10.1021/es032594m

Cited by 25 publications

(12 citation statements)

References 59 publications

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“…Contamination of sea water is being reported in the marine ecosystem due to xenobiotics used in pharmaceuticals and pesticides and from municipal sewage (Fent 1989;Aguilar and Borrell 2005). These alarming reports do not bode well for the health of the marine ecosystem and would endanger the health of the coral reefs and lead to degradation of this ecosystem We have previously reported the use of genomic tools to analyze environment impact assessment (Purohit et al 2003). Future studies in this area would be the analysis of the bacterial diversity data to predict the degradation of the coral reefs before visible damage sets in.…”

Section: Discussionmentioning

confidence: 99%

Preliminary analysis of bacterial diversity associated with the Porites coral from the Arabian sea

Kapley

Siddiqui

Misra

et al. 2007

World J Microbiol Biotechnol

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It is widely reported that coral reefs are suffering degradation from a combination of stresses. We have previously reported the use of genomic tools in the study of environmental impact assessment and hypothesize that monitoring the bacterial diversity associated with a coral would indicate changes in its health before visible damage occurs. This study analyzes the bacterial diversity associated with Porites coral collected from two sites in the Arabian Sea using culture independent techniques. Two clone libraries were constructed from the16S rDNA amplicons and selected individual clones were partially sequenced. Retrieved sequences were identified by BLAST analysis and indicate the presence of unidentified bacteria. Diversity index was calculated using Shannon-Wiener index. The bacterial diversity associated with coral sample 1 gives the index of species diversity, H 1 as 1.6287, divergence from eqiprobability as 45.708% and the evenness of the sample as 54.291. For coral sample 2, the values obtained were, H 1 as 1.97, divergence from eqiprobability as 15.11% and the evenness of the sample as 84.88. Sequences representing bacteria related to agricultural or industrial pollution and pathogenesis were also found. Coral samples collected near a lagoon area, showed a greater percentage of sequences representing bacteria related to human interventions, indicative of pollution.

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Section: Discussionmentioning

confidence: 99%

Preliminary analysis of bacterial diversity associated with the Porites coral from the Arabian sea

Kapley

Siddiqui

Misra

et al. 2007

World J Microbiol Biotechnol

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“…could be defi ned linguistically and the fuzzy numbers could be generated for each linguistic term. A fuzzy rule-based system could be developed that incorporates the knowledge about the role of different parameters in deciding the assimilatory capacity of the treatment plant [52].…”

Section: Looking Beyond the Culturable Microorganismsmentioning

confidence: 99%

Genomic tools in bioremediation

Kapley

Purohit

2009

Indian J Microbiol

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Bioremediation is a process that uses microorganisms or their enzymes to remove pollutants from the environment. Generally, bioremediation technologies can be classifi ed as in situ or ex situ. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be treated elsewhere. Like so much else in biology, the ease and availability of genomic data has created a new level of understanding this system. Bioremediation capabilities of the microbial population can be analyzed; not only by physiological parameters, but also by the use of genomic tools, and effi cient remediation strategies can be planned. PCR and DNA-or oligonucleotide-based microarray technology is a powerful functional genomics tool that allows researchers to view the physiology of a living cell from a comprehensive and dynamic molecular perspective. This paper explores the use of such tools in bioremediation process.

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“…Meanwhile, also in the USA, the National Academies of Sciences National Research Council Programme has reported on Applications of Toxicogenomic Technologies to Predictive Toxicology and Risk Assessment, making recommendations to realise the benefits of such an approach to investigation of interactions between environmental chemicals and genes in the human body and ultimately to practical utilisation by the regulatory agencies (NRC 2007). In some ways, this is analogous to the use of genomic tools in environmental impact assessment, where subtle impacts caused by industrial development that could have long-term effects can be monitored at the microbial level using selected tools (Purohit et al 2003). The difference with studies involving humans (e.g.…”

Section: A Local Case Study: Chromium-contaminated Land In Glasgowmentioning

confidence: 99%

Strategies for improving human health in contaminated situations: a review of past, present and possible future approaches

Farmer

Jarvis²

2008

Environ Geochem Health

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Strategies for improving human health in contaminated situations have traditionally been based on restricting emissions, remedial reduction of exposure and, where appropriate and possible, medical reconnaissance of efficacy. We review these and the broader aspects of general public health approaches, including necessary understanding of epidemiology and the wider social context, before considering a specific local case study involving health issues associated with chromium-contaminated land and its remediation in an area of urban regeneration. The impact of remediation upon the common good, in its broadest environmental, health and socio-economic sense, including enhanced opportunities for members of the community to take personal responsibility for health-improving activities, should be taken into account in addition to conventional theoretical assessments and practical measurements of relief from environmental risk. Rapidly emerging toxicogenomic technologies may have a role to play in informing future risk assessment and remediation approaches in contaminated situations, although the ethical challenges of using personal genetic information could well be considerable.

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Peer Reviewed: Genomics Tools in Environmental Impact Assessment

Cited by 25 publications

References 59 publications

Preliminary analysis of bacterial diversity associated with the Porites coral from the Arabian sea

Preliminary analysis of bacterial diversity associated with the Porites coral from the Arabian sea

Genomic tools in bioremediation

Strategies for improving human health in contaminated situations: a review of past, present and possible future approaches

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