Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations

Clicq, David; Pappaert, Kris; Vankrunkelsven, Sarah; Vervoort, Nico; Baron, Gino V.; Desmet, Gert

doi:10.1021/ac0416822

Cited by 26 publications

(31 citation statements)

References 27 publications

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“…18 The shear-driven flow channel is assembled by pressing two flat surfaces against each other. 18 The shear-driven flow channel is assembled by pressing two flat surfaces against each other.…”

Section: Methodsmentioning

confidence: 99%

“…18 Shear-driven systems have the potential to enhance chromatographic separations, 19,20 separation and transport of microparticles 21,22 and DNA-microarray hybridization kinetics. 18 Shear-driven systems have the potential to enhance chromatographic separations, 19,20 separation and transport of microparticles 21,22 and DNA-microarray hybridization kinetics.…”

Section: Introductionmentioning

confidence: 99%

“…In previous publications, our group has already reported on a number of potential applications and advantages of sheardriven microfluidic systems. 18 Shear-driven systems have the potential to enhance chromatographic separations, 19,20 separation and transport of microparticles 21,22 and DNA-microarray hybridization kinetics. 9,23 Shear driven flows are generated by mechanically moving the bottom half of a flat-rectangular channel past the top part (or vice versa).…”

Section: Introductionmentioning

confidence: 99%

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Measurements of diffusion coefficients in 1-D micro- and nanochannels using shear-driven flows

et al. 2005

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The present paper describes a method for measuring the molecular diffusion coefficient of fluorescent molecules in microfluidic systems. The proposed static shear-driven flow method allows one to perform diffusion measurements in a fast and accurate manner. The method also allows one to work in very thin (i.e. submicron) channels, hence allowing the investigation of diffusion in highly confined spaces. In the deepest investigated channels, the obtained results were comparable to the existing literature values, but when the channel size dropped below the micrometer range, a significant decrease (more than 30%) in molecular diffusivity was observed. The reduction of the diffusivity was most significant for the largest considered molecules (ssDNA oligomers with a size ranging between 25 to 100 bases), but the decrease was also observed for smaller tracer molecules (FITC). This decrease can be attributed to the interactions of the analyte molecules with the channel walls, which can no longer be neglected when the depth of the channel reaches a critical value. The change in diffusivity seems to become more explicit as the molecular weight of the analytes increases.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Measurements of diffusion coefficients in 1-D micro- and nanochannels using shear-driven flows

et al. 2005

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“…Fine stationary phase particles, which allow a reduction of the time required for radial diffusion of a solute, are often employed to gain a high separation performance. 12,13 Desmet et al [14][15][16][17][18] devised sheardriven flow chromatography, in which a mobile phase is sandwiched between two plates and the movement of one wall causes a shear-driven flow of the mobile phase. In their study, the surface of a fixed wall was modified to act as a stationary phase.…”

Section: Introductionmentioning

confidence: 99%

Shear-Driven Flow Ice Chromatography as a Possible Tool Probing Ice/Water Interface

et al. 2016

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Although ice chromatography is a useful probe of ice interfaces, its low separation efficiency has often made difficult to access the ice/water interface. Coupling of this method with shear-driven flow chromatography, which has high separation potential, solves the problems involved in ice chromatography. This paper reports on shear-driven flow ice chromatographic instrumentation, and discusses the separation performance. Electrostatic separation of positively and negatively charged dyes is demonstrated with an OH --doped ice plate as a stationary phase.Keywords Shear flow, ice chromatography, electrostatic interaction at ice interface, interface between ice and aqueous solution

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“…Component A has a higher affinity to the stationary phase compared with component B. After a time ∆t both components are clearly separated from each other [1] [2]. The generation of the liquid flow in micro-channels can be achieved by using pressure, electrical or shear-driven systems.…”

Section: Introductionmentioning

confidence: 99%

A novel optical detection system for chromatography applications

et al. 2006

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In this work we describe the design of a confocal microscope based optical system which can be used for two types of miniaturized fluid-chromatographic experiments: first of all for the study of shear-driven fluid separations in microchannels and secondly for the study of peak-broadening phenomena in pressure-driven fluids in the same type of microchannels. For the first type of measurements fluorescing dyes (coumarins) are used while for the latter type photo-active molecules (uncaged dyes) like for example fluorescein molecules are needed. We designed the detection system in such a way that by making some small changes to the system we can swap from one application to the other. After calculating the specifications of the optical components, we simulated the system with the sequential ray-tracing-software Solstis and built the system. Finally we did some preliminary experiments which demonstrated the working principle of our setup.

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Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations

Cited by 26 publications

References 27 publications

Measurements of diffusion coefficients in 1-D micro- and nanochannels using shear-driven flows

Measurements of diffusion coefficients in 1-D micro- and nanochannels using shear-driven flows

Shear-Driven Flow Ice Chromatography as a Possible Tool Probing Ice/Water Interface

A novel optical detection system for chromatography applications

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