Peer Reviewed: Thermal Lens Microscopy and Microchip Chemistry

Kitamori, T.; Tokeshi, Manabu; Hibara, Akihide; Sato, Katsuaki

doi:10.1021/ac041508d

Cited by 201 publications

(174 citation statements)

References 26 publications

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“…Since there is a linear dependence on the concentration of the absorbing molecules, quantitative analysis can be carried out by measuring changes in the divergence or convergence of the probe beam due to the thermal lensing. Two reviews [58,59] and an excellent paper [60] have been recently published that collectively provide a comprehensive overview of the technique and instrumentation involved for microchip thermal lens microscopy (TLM).…”

Section: Thermal Lens Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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The direction of modern analytical techniques is to push for lower detection limits, improved selectivity and sensitivity, faster analysis time, higher throughput, and more inexpensive analysis systems with ever-decreasing sample volumes. These very ambitious goals are exacerbated by the need to reduce the overall size of the device and the instrumentation -the quest for functional micrototal analysis systems epitomizes this. Microfluidic devices fabricated in glass, and more recently, in a variety of polymers, brings us a step closer to being able to achieve these stringent goals and to realize the economical fabrication of sophisticated instrumentation. However, this places a significant burden on the detection systems associated with microchip-based analysis systems. There is a need for a universal detector that can efficiently detect sample analytes in real time and with minimal sample manipulation steps, such as lengthy labeling protocols. This review highlights the advances in uncommon or less frequently used detection methods associated with microfluidic devices. As a result, the three most common methods -LIF, electrochemical, and mass spectrometric techniques -are omitted in order to focus on the more esoteric detection methods reported in the literature over the last 2 years.

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Section: Thermal Lens Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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“…The TLM system has been described elsewhere. 4,11 Briefly, the TLM system consisted of an optical microscope (Special design, Nikon, Tokyo, Japan), two lasers (a diode pumped Nd:YVO4 laser (JUNO 100, 532 nm, 100 mW, Showa Optronics Co., Ltd., Tokyo, Japan) for the excitation beam and a He-Ne laser (05LHP171, 632.8 nm, 15 mW, Melles Griot, Carlsbad, CA) for the probe beam) and opto-electronic detection systems. The excitation beam, which was mechanically modulated at 1020 Hz by a light chopper (5584A, NF Corporation, Yokohama, Japan), and the probe beam were coaxially aligned by a dichroic mirror and a mirror in the bodytube of the microscope, and then introduced into an objective lens with a numerical aperture of 0.46 and 20 magnification (CF IC EPI Plan, Nikon).…”

Section: Measurementsmentioning

confidence: 99%

“…We have developed a thermal lens microscope (TLM) that is thermal lens spectroscopy realized under a microscope. 4 With TLM, the highly sensitive detection of a wide variety of compounds in a micro space, such as in a micro chemical chip, has become possible. We have successfully demonstrated many applications of TLM to microchips.…”

Section: Introductionmentioning

confidence: 99%

Optimization of an Interface Chip for Coupling Capillary Electrophoresis with Thermal Lens Microscopic Detection

et al. 2005

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“…volume measurements) and transport to the detector, while microfluidic lab-on-a-chip technology can facilitate and speed up processes, including colorimetric reactions, antigen-antibody or enzyme-substrate interactions in bioanalytical systems, and even the extraction and preconcentration steps by introducing continuous-flow processing and micro unit operations in chemical analysis. 9 A crucial advantage of microfluidic systems lies in the fact that small dimensions of such systems, which consist of capillaries and micro reactors with dimensions of about 10 to 100 μm, significantly reduce the molecular diffusion time, which is inversely proportional to the second power of distance. For example, the time required for the completion of an ELISA immunoassay for immunoglobulin A, performed in a commercial microtiter plate (dimensions of a well are of several mm), was reduced from 15 h to only 10 min when transferring the assay into a microfluidic system, while maintaining, or even improving, the sensitivity.…”

Section: Introductionmentioning

confidence: 99%