PEG-Based Hydrogel Synthesis via the Photodimerization of Anthracene Groups

Zheng, Y. George; Mićić, Miodrag; Mello, Sarita V.; Mabrouki, Mustapha; Andreopoulos, Fotios M.; Konka, Veeranjaneyulu; Pham, Si M.; Leblanc, Roger M.

doi:10.1021/ma012263z

Cited by 162 publications

(130 citation statements)

References 36 publications

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“…Given that PEG is highly hydrophilic and generally nonadhesive to proteins or cells, it has found widespread use as a drug carrier [14], and many PEG hydrogels have been produced from aqueous solutions containing linear or branched PEG macromolecules via chemical crosslinking [15][16][17][18]. In addition to hydrogels formed via radical crosslinking reactions, PEG hydrogels have been formed, for example, via Michael-type addition reactions upon mixing with thiol-bearing compounds [19,20] or via the reaction between amino-terminated poly (ethylene glycol) and the herbal iridoid glycoside genipin [21].…”

Section: Introductionmentioning

confidence: 99%

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Zhang

Furst

Kiick

2006

Journal of Controlled Release

117

131

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The design of materials in which assembly, mechanical response, and biological properties are controlled by protein-polysaccharide interactions could provide materials that mimic the biological environment and find use in the delivery of growth factors. In the investigations reported here, a heparin-binding, coiled-coil peptide, PF4 ZIP , was employed to mediate the assembly of heparinized polymers. The heparin-binding affinity of this peptide was compared with that of other heparinbinding peptides (HBP) via heparin-sepharose chromatography and surface plasmon resonance (SPR) experiments. Results from these experiments indicate that PF4 ZIP demonstrates a higher heparin-binding affinity and heparin association rate when compared to the heparin-binding domains of antithrombin III (ATIII) and heparin-interacting protein (HIP). Viscoelastic hydrogels were formed upon the association of PF4 ZIP -functionalized star poly(ethylene glycol) (PEG-PF4 ZIP ) with low-molecular-weight heparin-functionalized star PEG (PEG-LMWH). The viscoelastic properties of the hydrogels can be altered via variations in the ratio of LMWH to PF4 ZIP . bFGF release from these gels have also been investigated. Comparison of the bFGF release profiles with the hydrogel erosion profiles indicates that bFGF delivery from this class of hydrogels is mainly an erosioncontrolled process and the rates of bFGF release can be modulated via choice of HBP or via variations in the mechanical properties of the hydrogels. Manipulation of hydrogel physical properties and erosion profiles will provide novel materials for controlled growth factor delivery and other biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Zhang

Furst

Kiick

2006

Journal of Controlled Release

117

131

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“…As tRNA is a weak fluorophore, the titration of mPEGanthracene was done against various tRNA concentrations, using mPEG-anthracene excitation at 330-350 nm and emission at 400-450 nm [41,42]. When mPEG-anthracene interacts with tRNA, fluorescence may change depending on the impact of such interaction on the mPEG-anthracene conformation or via direct quenching effect [43].…”

Section: Stability Of Synthetic Polymer-trna Complexesmentioning

confidence: 99%

Effect of Synthetic Polymers on tRNA Nanoparticle Formation

HA¹

2015

JNMR

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In this review, we have examined the effects of several synthetic nanoparticles such as poly (ethylene glycol) PEG-(PEG-3350, PEG-6000), methoxypoly (ethylene glycol) anthracene (mPEG-anthracene), methoxypoly (ethylene glycol) poly (amidoamine) (mPEG-PAMAM-G3), (mPEG-PAMAM-G4) and poly (amidoamine) (PAMAM-G4) on tRNA structure and dynamics. Atomic force microscopic (AFM) images and spectroscopic data were analysed and the effects of synthetic polymer complexation on tRNA stability, aggregation and particle formation are discussed. Hydrophilic and hydrophobic contacts were dominated in the polymer-tRNA complexation and the overall binding constants showed that the order of binding is PEG-6000>PAMAM-G4>PEG-3350>mPEG-PAMAM-G4>mPEG-PAMAM-G3>mPEG-anthracene. The morphology of polymer-tRNA complexes showed major aggregation and nanoparticle formation of tRNA, in the presence of synthetic nanoparticles.

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“…Since DNA is a weak fluorophore, the titration of mPEGanthracene was done against various DNA concentrations, using mPEG-anthracene excitation at 330-350 nm and emission at 400-450 nm [35,36]. When mPEG-anthracene interacts with DNA, fluorescence may change depending on the impact of such interaction on the mPEG-anthracene conformation or via direct quenching effect [37].…”

Section: The Number Of Binding Sites Occupied By Polymer On Dna Duplexmentioning

confidence: 99%

DNA Compaction and Particle Formation by Synthetic Polymers: Applications in Gene Delivery

HA¹

2015

JNMR

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ExperimentalAtomic force microscopy: Polymer-DNA complexes at a ratio of 1:1 and final DNA concentration of 0.1 mM were prepared in 5 ml Tris-HCl (pH 7.4). The solutions were either used undiluted or diluted further in ultrapure water. For each sample, 30 µl aliquot was adsorbed for two minutes on freshly cleaved muscovite mica. The surface was rinsed thoroughly with 10 ml of ultrapure water and dried with Argon. AFM imaging was performed in acoustic mode at a scanning speed of 1Hz with an Agilent 5500 (Agilent, Santa Barbara, CA) using high frequency (300 kHz) silicon cantilevers with a tip radius of 2-5 nm (TESP-SS, Veeco, Santa Barbara, CA) [26]. Images were treated using the software Gwyddion (http://gwyddion.net/). FTIR spectroscopy: Infrared spectra were recorded on a FTIR spectrometer (Impact 420 model), equipped with DTGS (deuterated triglycine sulfate) detector and KBr beam splitter, using AgBr windows. Spectra were collected after 2h incubation of polymer with the DNA solution and measured. Interferograms were accumulated over the spectral range 4000-600 cm -1 with a nominal resolution of 2 cm -1 and a minimum of 100 scans. The difference spectra [(DNA solution + polymer) -(DNA solution)] were obtained, using a sharp band at 968 (DNA) as internal reference. This band, which is due to sugar C-C stretching vibrations, exhibits no spectral changes (shifting or intensity variations) upon polymer-polynucleotide complexation, and cancelled out upon spectral subtraction [26].CD spectroscopy: The CD spectra of DNA and its polymer adducts were recorded at pH 7.3 with a Jasco J-720 spectropolarimeter. For measurements in the Far-UV region (200-320 nm), a quartz cell with a path length of 0.01 cm was used. Six scans were accumulated at a scan speed of 50 nm per minute, with data being collected at every nm from 200 to 320 nm. Sample temperature was maintained at 25 °C using a Neslab RTE-111Submit Manuscript | http://medcraveonline.com J Nanomed Res 2015, 2(4): 00038 AbstractWe have reviewed the effects of synthetic polymers on DNA compaction and particle formation. Synthetic polymers such as poly (ethylene glycol) PEG-(PEG-3350, PEG-6000), methoxypoly (ethylene glycol) anthracene (mPEGanthracene), methoxypoly (ethylene glycol) poly (amidoamine) (mPEG-PAMAM-G3), (mPEG-PAMAM-G4) and poly(amidoamine) (PAMAM-G4) alter DNA structure and dynamic. The spectroscopic results and atomic force microscopic (AFM) were analysed and the effect of synthetic polymer complexation on DNA stability, aggregation, compaction and particle formation are discussed. A comparison of the overall binding constants showed that the order of binding PAMAM-G4>PEG-6000>PEG-3350>mPEG-anthracene> mPEG-PAMAMG4>mPEG-PAMAM-G3. The morphology and ultrastructure of polymer-DNA adducts showed major DNA compaction and particle formation induced by synthetic polymers. The generated information is useful for the application of synthetic nanoparticles in gene delivery.

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PEG-Based Hydrogel Synthesis via the Photodimerization of Anthracene Groups

Cited by 162 publications

References 36 publications

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Effect of Synthetic Polymers on tRNA Nanoparticle Formation

DNA Compaction and Particle Formation by Synthetic Polymers: Applications in Gene Delivery

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