Peg-Bovine Hemoglobin: Safety en a Canine Dehydrated Hypovolemic-Hemorrhagic Shock Model

Abstract

An initial evaluation of PEG-bHb was performed using a modified hypovolemic shock model. PEG-bHb had a substantially longer intravascular half-life than native Hb and no measurable hemoglobinuria was observed in the canine. PEG-bHb allowed successful resuscitation with an oxygen carrying capacity of 14-22% over that of lactated Ringer's solution.

Carbon monoxide form of PEGylated hemoglobin protects myocardium against ischemia/reperfusion injury in diabetic and normal mice

“…Surface lysine residues on purifi ed bovine Hb were conjugated with 5000 molecular weight PEG (Nho et al, 1992). Th e PEG-Hb solution was bubbled with CO to convert greater than 80% of the PEG-Hb to PEG-COHb before storage.…”

Sanguinate's effect on pial arterioles in healthy rats and cerebral oxygen tension after controlled cortical impact

“…e l s e v i e r . c o m / l o c a t e / y m v r e 5000-molecular weight residues of polyethylene glycol (PEG) on the surface lysine residues (Nho et al, 1992) in phosphate-buffered saline. It is a 4% Hb solution with an osmolarity of 310-360 mOsm, and a P 50 (PO 2 at 50% oxyhemoglobin saturation) of ∼ 11 mm Hg.…”

Hemoglobin-Based Blood Substitutes and the Treatment of Sickle Cell Disease: More Harm than Help?

“…Sanguinate is a purified bovine Hb manufactured by Prolong Pharmaceuticals (Piscataway, NJ, USA) and is conjugated with 5000 molecular weight of PEG residues on the surface lysines and is ligated to CO [57]. The unligated form of this HBOC has a high oxygen affinity (a P 50 of approximately 11 mmHg).…”

“…A Phase I/II study using polymerized bovine hemoglobin (HBOC-201, Hemopure) was carried out in adult patients with sickle cell disease who were not at a crisis at the time of the study [57]. In this investigation, 18 adults with SCD who were asymptomatic at the time were enrolled for this study.…”