PEG infiltration: an alternative method to obtain thin sections of cacti tissues

Mozzi, Giacomo; Romero, Eunice; Martínez-Quezada, Daniel M.; Crivellaro, Alan

doi:10.1163/22941932-bja10051

Cited by 9 publications

(6 citation statements)

References 28 publications

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“…Studies have been hampered by the challenges of applying standard histological and biochemical techniques to water-rich tissues, with methodological modifications often being required in order to investigate succulent tissues (e.g. Ahl et al , 2018 ; Mozzi et al , 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Elastic and collapsible: current understanding of cell walls in succulent plants

Fradera‐Soler

Grace

Jørgensen

et al. 2022

Journal of Experimental Botany

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Succulent plants represent a large functional group of drought-resistant plants which store water in specialized tissues. Several co-adaptive traits accompany water-storage capacity to constitute the succulent syndrome. A widely reported anatomical adaptation of cell walls in succulent tissues allows them to fold in a regular fashion during extended drought, thus preventing irreversible damage and allowing for reversible volume changes. Although ongoing research on crop and model species continuously reports the importance of cell walls and their dynamics in drought resistance, cell walls of succulent plants have received relatively little attention to date, despite the potential of succulents as natural capital to mitigate the effects of climate change. In this review, we summarize the current knowledge of cell walls in drought-avoiding succulents and their effects on tissue biomechanics, water relations and photosynthesis. We also highlight the existing knowledge gaps and propose a hypothetical model for regulated cell wall folding in succulent tissues upon dehydration. Future perspectives of methodological development in succulent cell wall characterization, including the latest technological advances in molecular and imaging techniques, are also presented.

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Section: Introductionmentioning

confidence: 99%

Elastic and collapsible: current understanding of cell walls in succulent plants

Fradera‐Soler

Grace

Jørgensen

et al. 2022

Journal of Experimental Botany

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“…PEG infiltration is widely used for archaeological wood to stabilize cell structure, while paraffin-embedding of tree-ring microcores and bark tissue represents a good embedding effect (Rossi et al 2006;Hamann et al 2011;Pace et al 2019;Mozzi et al 2021). Compared to PEG embedding, paraffin needs to be removed by HistoClear or limonene before staining and imaging (Yeung et al 2015;Mozzi et al 2021). High molecular weight PEG was selected in our experiment to impregnate the G. biloba stem xylem.…”

Section: Discussionmentioning

confidence: 99%

Practical guidelines for quantitative wood anatomy on Ginkgo biloba L.

Huang

2022

IAWA J.

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Summary Ginkgo biloba L., a long-lived relict species, has high ecological, cultural and economic value. Studying the quantitative wood anatomy of G. biloba is important to understand the relationships between their tree-growth and climate change, which could help in the protection of old G. biloba trees. Preparing high-quality wood thin sections is the premise of quantitative wood anatomy analysis. In addition, manual correction of image analysis is a time-consuming process. We therefore present a protocol on how to make G. biloba high-quality xylem thin sections, from sample preparation, sample embedding, blade selection to thickness selection. We also present a method to determine the necessary sample width and number of cores to establish reliable wood anatomical time series that both can capture climate signals and reduce the time used for image analysis. For microsection preparation, it is optimal to cut 20–30-μm-thick sections using Leica DB80 blades after applying the cornstarch solution on top of the sample surface. For image analysis, the reliable wood anatomical time series can be established when measuring six cores (trees) or above, and the environmental signals are captured when analyzing a 1.2-mm-wide radial strip along the increment core (deviation <2%) for the parameters cell density, mean lumen area, potential hydraulic conductivity and percentage of the conductive area. This paper provides practical guidance to successfully use quantitative wood anatomy methods on G. biloba. We hope our study will be helpful for future research on the quantitative wood anatomy of G. biloba and similar tree species.

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“…The advantages of PEG 1500 are that it is water soluble and does not make lignified samples harder. PEG 1500 has in addition excellent penetration, and large samples can be embedded, even entire branches [ 87 , 88 ]. If working in a very humid environment, the addition of a 10% PEG 4000 on the last stage of the process helps making the block less prone to absorbing room humidity and easier to handle.…”

Section: Overcoming the Methodological Limitationmentioning

confidence: 99%

Priorities for Bark Anatomical Research: Study Venues and Open Questions

Shtein

Gričar

Lev‐Yadun

et al. 2023

Plants

Self Cite

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The bark fulfils several essential functions in vascular plants and yields a wealth of raw materials, but the understanding of bark structure and function strongly lags behind our knowledge with respect to other plant tissues. The recent technological advances in sampling and preparation of barks for anatomical studies, along with the establishment of an agreed bark terminology, paved the way for more bark anatomical research. Whilst datasets reveal bark’s taxonomic and functional diversity in various ecosystems, a better understanding of the bark can advance the understanding of plants’ physiological and environmental challenges and solutions. We propose a set of priorities for understanding and further developing bark anatomical studies, including periderm structure in woody plants, phloem phenology, methods in bark anatomy research, bark functional ecology, relationships between bark macroscopic appearance, and its microscopic structure and discuss how to achieve these ambitious goals.

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PEG infiltration: an alternative method to obtain thin sections of cacti tissues

Cited by 9 publications

References 28 publications

Elastic and collapsible: current understanding of cell walls in succulent plants

Elastic and collapsible: current understanding of cell walls in succulent plants

Practical guidelines for quantitative wood anatomy on Ginkgo biloba L.

Priorities for Bark Anatomical Research: Study Venues and Open Questions

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