PEG-mediated osmotic stress induces premature differentiation of the root apical meristem and outgrowth of lateral roots in wheat

Ji, Hongtao; Liu, Ling; Li, Kexue; Qingen, Xie; Wang, Zhijuan; Zhao, Xiuhua; Li, Xia

doi:10.1093/jxb/eru255

Cited by 89 publications

(76 citation statements)

References 33 publications

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“…Six day-old mutants defective in ABA biosynthesis (aba2-1) and the signaling pathway (the triple mutant of ABA receptors pyr1/pyl1pyl4) were subjected to 5% PEG 8000 simulated water stress as described previously. 3 As shown in, Fig. 2A root tips of the aba2-1 and pyr1/ pyl1pyl4 mutants were morphologically comparable to the wild type under normal conditions.…”

Section: Aba and Signaling Pathway Affect Ram Premature Differentiatimentioning

confidence: 59%

“…2 Recently, we reported that under mild and moderate water stresses, plant primary roots undergo root tip swelling and RAM premature differentiation resulting in cessation of the primary root growth. 3 We demonstrated that the RAM premature differentiation of primary roots and subsequent lateral formation are directly correlated to plant stress tolerance. Importantly, we provide evidence that the RAM premature differentiation and remodeling of root system architecture induced by mild and moderate water stresses simulated by PEG8000 is a conserved adaptive mechanism that is well adopted by various plants to cope with water stress.…”

Section: Introductionmentioning

confidence: 82%

“…2A) were much less than that under PEG8000. 3 Thus, despite the critical role of ABA in RAM activity maintenance, it is conceivable that RAM activity and root growth under water stress are coordinated by crosstalk of several hormonal signaling pathways, especially the ABA and auxin signaling pathways. It is likely that the ratio of ABA and auxin fine tunes activity of RAM and growth rates of roots under water stress.…”

Section: Discussionmentioning

confidence: 99%

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ABA mediates PEG-mediated premature differentiation of root apical meristem in plants

2014

Plant Signaling & Behavior

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Abbreviations: PEG8000, polyethylene glycol; RAM, root apical meristem; ABA, abscisic acid.Root apical meristem (RAM) is central for indeterminate growth of plant roots and in sensing environmental stimuli, such as water status. Recently, we reported that PEG8000-simulated mild and moderate osmotic stress induces premature differentiation of RAM, which is a conserved adaptive mechanism in higher plants to cope with water stress. Microarray data analysis revealed that the ABA signaling pathway may be involved in water stress-induced RAM premature differentiation. Here we showed that in wheat, ABA contents increased under water stress with the highest level of ABA in the RAM. Exogenous ABA also induces RAM premature differentiation in both wheat and Arabidopsis plants. Further genetic analysis revealed that loss of function mutations in ABA2 and ABA receptors significantly reduced the level of root tip swelling and RAM premature differentiation in response to PEG-simulated water stress. Together, the results suggest that ABA participates in regulation of PEG-mediated premature differentiation of RAM.

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Section: Aba and Signaling Pathway Affect Ram Premature Differentiatimentioning

confidence: 59%

Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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ABA mediates PEG-mediated premature differentiation of root apical meristem in plants

2014

Plant Signaling & Behavior

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“…Extensive research efforts over the last five decades revealed physiological mechanisms relevant to osmotic stress response of crop plants [8][9][10][11][12][13]. Awareness related to root morphology of annual crop plants, such as wheat, barley, or perennial forage grasses under limited water supply, has developed within the last 20 years [14][15][16][17][18][19][20][21][22][23]. However, little awareness has been created as to how root hairs get modified, and are possibly associated with crop yield under different levels of osmotic potential.…”

Section: Introductionmentioning

confidence: 99%

Polyethylene Glycol (PEG)-Treated Hydroponic Culture Reduces Length and Diameter of Root Hairs of Wheat Varieties

2015

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Abstract:Wheat is an important cereal crop worldwide that often suffers from moisture deficits at the reproductive stage. Polyethylene glycol (PEG)-treated hydroponic conditions create negative osmotic potential which is compared with moisture deficit stress. An experiment was conducted in a growth chamber to study the effects of PEG on root hair morphology and associated traits of wheat varieties. Plants of 13 wheat varieties were grown hydroponically and three different doses of PEG 6000 (w/v): 0% (control), 0.3% and 0.6% (less than −1 bar) were imposed on 60 days after sowing for 20 days' duration. A low PEG concentration was imposed to observe how initial low moisture stress might affect root hair development. PEG-treated hydroponic culture significantly decreased root hair diameter and length. Estimated surface area reduction of root hairs at the main axes of wheat plants was around nine times at the 0.6% PEG level compared to the control plants. Decrease in root hair diameter and length under PEG-induced culture decreased "potential" root surface area per unit length of main root axis. A negative association between panicle traits, length and dry weight and the main axis length of young roots indicated competition for carbon during their development. Data provides insight into how a low PEG level might alter root hair development.

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“…Lower concentrations of NaCl reduced root growth, which was also reflected in the reduction of the size of the root meristem (Hanif andDavies 1998, Zadeh 2007). Recently, a reduction in root meristem size was also reported for wheat under PEG-mediated osmotic stress (Ji et al 2014). However, the mechanism by which the stress affects the meristem length is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Allium cepa root meristem cells under osmotic (sorbitol) and salt (NaCl) stress in vitro

Kiełkowska¹

2017

Acta Botanica Croatica

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-The effects of various concentrations of sorbitol (100, 200 and 360 mM) and NaCl (100, 200 and 300 mM) on root meristem cells of in vitro-cultured Allium cepa L. were analyzed after 10 and 20 days. Both root meristem cell cross-section area and nuclear volume decreased under osmotic and salt stress. The osmotic component of applied stresses had a greater impact on cell shrinkage, while ionic stress perturbed cell functioning, resulting in cell cycle arrest and various aberrations, affecting nucleus integrity. A concentration of 300 mM of NaCl in the culture medium caused complete inhibition of mitotic activity in onion root tip cells after 20 days of exposure. Analysis of the action of iso-osmotic concentrations of NaCl (200 mM) and sorbitol (360 mM) showed stronger mitodepressive effects of salt stress in comparison to osmotic stress.

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PEG-mediated osmotic stress induces premature differentiation of the root apical meristem and outgrowth of lateral roots in wheat

Abstract: SummaryPEG-mediated osmotic stress induces premature differentiation of the root apical meristem, which in turn leads to outgrowth of lateral roots and improved tolerance to water stress.

Cited by 89 publications

References 33 publications

ABA mediates PEG-mediated premature differentiation of root apical meristem in plants

ABA mediates PEG-mediated premature differentiation of root apical meristem in plants

Polyethylene Glycol (PEG)-Treated Hydroponic Culture Reduces Length and Diameter of Root Hairs of Wheat Varieties

Allium cepa root meristem cells under osmotic (sorbitol) and salt (NaCl) stress in vitro

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