PEGylated Calcium Phosphate Nanocomposites as Smart Environment‐Sensitive Carriers for siRNA Delivery

Zhang, Mingzhen; Ishii, Atsushi; Nishiyama, Nobuhiro; Matsumoto, Satoru; Ishii, Takehiko; Yamasaki, Yuichi; Kataoka, Kazunori

doi:10.1002/adma.200800448

Cited by 99 publications

(81 citation statements)

References 28 publications

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“…[43][44][45] To a large extent, this is due the well-known biopassivation abilities of PEO 46 and the strong interaction of poly(electrolyte)s with inorganic ions [47][48][49][50] and surfaces. 47,48,[51][52][53] The combination of highly charged remineralizition-enhancing 48,49,[54][55][56][57][58][59][60][61][62] or dissolution-limiting 63,64 polymer segments with the biopassivation of PEO is thus a viable strategy towards multifunctional polymers providing both anti-biolm and remineralization-enhancing behavior in one single system.…”

Section: 26mentioning

confidence: 99%

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

et al. 2015

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The present article is among the first reports on the effects of poly(ampholyte)s and poly(betaine)s on the biomimetic formation of calcium phosphate. We have synthesized a series of di-and triblock copolymers based on a non-ionic poly(ethylene oxide) block and several charged methacrylate monomers, 2-(trimethylammonium)ethyl methacrylate chloride, 2-((3-cyanopropyl)-dimethylammonium)ethyl methacrylate chloride, 3-sulfopropyl methacrylate potassium salt, and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide. The resulting copolymers are either positively charged, ampholytic, or betaine block copolymers. All the polymers have very high molecular weights of over 10 6 g mol À1 . All polymers are water-soluble and show a strong effect on the precipitation and dissolution of calcium phosphate.The strongest effects are observed with triblock copolymers based on a large poly(ethylene oxide) middle block (nominal M n ¼ 100 000 g mol À1 ). Surprisingly, the data show that there is a need for positive charges in the polymers to exert tight control over mineralization and dissolution, but that the exact position of the charge in the polymer is of minor importance for both calcium phosphate precipitation and dissolution.

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Section: 26mentioning

confidence: 99%

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

et al. 2015

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“…PEG-bp-CaP showed effectively and sustained transfection ability to cells in vitro with low toxicity. Zhang et al [18] developed CaP nanomicellar carriers which can efficiently keep siRNA in their CaP inner core by using PEG for regulating the size of the nanoparticles, which facilitated the internalization of siRNA by cells. The nanoparticles exhibited a well-defined spherical shape with a diameter of 100 nm and the thickness of PEG shell is about 10 nm.…”

Section: The Use Of Calcium Phosphate Nanoparticles For Gene Silencingmentioning

confidence: 99%

“…[17]. HA nanoparticles have recently been used in medical applications as carriers for growth factors, antibiotics, cancer drugs, antigens, and nucleic acids [18]. Moreover, HA columns are used for protein chromatography [19].…”

Section: Calcium Phosphatesmentioning

confidence: 99%

Gene Delivery by Hydroxyapatite and Calcium Phosphate Nanoparticles: A Review of Novel and Recent Applications

Bakan¹

2018

Hydroxyapatite - Advances in Composite Nanomaterials, Biomedical Applications and Its Technological Facets

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Gene therapy is a targeted therapy which can be used in the treatment of various acquired and inherited diseases. Inhabitation of a gene function, restoring or improving a gene, or gaining a new function can be achieved by gene therapy strategies. The most crucial step in this therapy is delivering the therapeutic material to the target. Nanosized calcium phosphates (CaPs) have been considered as promising carriers due to their excellent biocompatibility. In this chapter, the delivery of DNA, siRNA, and miRNA by using CaP nanocarriers were compiled in detail and the main parameters which can affect the carrier properties and thus the gene transfer efficiency were also discussed.

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“…The artificial HA is similar to the body's own HA with an excellent biocompatibility, bioactivity, biodegradability and non-immunogenicity vivo and vitro [14,15]. Research suggests that the inhibitory of HA had dose-dependent manner, which was better with the increasing of HA concentration.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite

Chen¹,

Xu²,

Wang³

et al. 2017

Proceedings of the 2017 International Conference on Material Science, Energy and Environmental Engineering (MSEEE 2017)

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Abstract. The exploration of the apoptosis for HepG2 cells induced by low-frequency and low-power ultrasound combined with hydroxyapatite, provides data base for future clinical treatment. In this research, we selected the HepG2 cells model. The experiences were divided into four groups: control group, ultrasound group, hydroxyapatite group, and ultrasound + hydroxyapatite group. Ultrasound group and control group replaced the normal culture medium, hydroxyapatite group and ultrasound + hydroxyapatite group replaced culture medium containing hydroxyapatite. The control group and hydroxyapatite group were not treated, while ultrasound group and ultrasound + hydroxyapatite group were treated by ultrasound with different parameters (f= 20 kHz; P=0.1, 0.2, 0.3w/cm 2 ; t=30, 60, 90, 120, 150, 180s; T= 0, 12, 24, 48, 72h). MTT test and flow cytometry detected the inhibitory rate of the cells, cell apoptosis and cell cycle. MTT experiment results show that ultrasound with different parameters stimulates the cell model, which leads to the difference of the inhibition rate of cell growth. Among them, the inhibition rate of cell growth reached the top value (71.726±0.677)% with the treatment of f=20 kHz, P =0.2w/cm 2 ,t=90s,T=48h. There was a statistical significance compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05).Under such condition, the apoptosis rate of HepG2 cells was a statistically significant change compared with the control group, ultrasound group, and hydroxyapatite group (P<0.05). During the cell cycle assay, the ultrasonic + hydroxyapatite group also showed obvious G2/M phase block. Low frequency and low-power ultrasound with Hydroxyapatite induced the apoptosis in human HepG2 cells.

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PEGylated Calcium Phosphate Nanocomposites as Smart Environment‐Sensitive Carriers for siRNA Delivery

Cited by 99 publications

References 28 publications

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

Gene Delivery by Hydroxyapatite and Calcium Phosphate Nanoparticles: A Review of Novel and Recent Applications

Apoptosis for HepG2 Cells Induced by Low-frequency and Low-power Ultrasound with Hydroxyapatite

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