PEGylated carboxymethyl chitosan/calcium phosphate hybrid anionic nanoparticles mediated hTERT siRNA delivery for anticancer therapy

Xie, Ying; Qiao, Hongzhi; Su, Zhigui; Chen, Minglei; Ping, Qineng; Sun, Minjie

doi:10.1016/j.biomaterials.2014.05.068

Cited by 140 publications

(82 citation statements)

References 45 publications

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“…siRNA can inhibit production of oncogenic regulators associated with tumor growth, transformation, and metastasis by a sequence-specific gene silencing effect [1-4]. The introduced siRNA interacts with the RNA-induced silencing complex (RICS) in the cell cytoplasm and promotes cleavage of messenger RNA (mRNA) which consists of complementary sequences [5-7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of dendrimer type bio-reducible polymer as a siRNA delivery carrier for cancer therapy

Nam

Jung

et al. 2015

Journal of Controlled Release

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Small interfering ribonucleic acid (siRNA), 20 – 25 base pairs in length, can interfere with the expression of specific genes. Recently, many groups reported the therapeutic intervention of siRNA in various cancer cells. In this study, dendrimer type bio-reducible polymer (PAM-ABP) which was synthesized using arginine grafted bio-reducible poly(cystaminebisacrylamide-diaminohexane) (ABP) and polyamidoamine (PAMAM) was used to deliver anti-VEGF siRNA into cancer cell lines including human hepatocarcinoma (Huh-7), human lung adenocarcinoma (A549), and human fibrosarcoma (HT1080) cells and access their potential as a siRNA delivery carrier for cancer therapy. PAM-ABP and siRNA formed polyplexes with average diameter of 116 nm and charge of around +24.6 mV. The siRNA in the PAM-ABP/siRNA polyplex released by 5 mM DTT and heparin. VEGF gene silencing efficiency of PAM-ABP/siRNA polyplexes was shown to be more effective than PEI/siRNA polyplexes in three cell lines with the following order HT1080>A549>Huh-7.

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Section: Introductionmentioning

confidence: 99%

Evaluation of dendrimer type bio-reducible polymer as a siRNA delivery carrier for cancer therapy

Nam

Jung

et al. 2015

Journal of Controlled Release

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“…Among the polymers commonly employed as non-viral si-RNA delivery systems, chitosan (Ma et al, 2014;Ozpolat et al, 2014;Xie et al, 2014), poly(ethylene imine) (Urban-Klein et al, 2005;Nabzdyk et al, 2014), poly(amidoamine) dendrimers (Patil et al, 2008;Conti et al, 2014) and poly(L-lysine) (Inoue et al, 2008;Kodama et al, 2014) have been extensively investigated.…”

Section: Introductionmentioning

confidence: 99%

EpCAM-targeted liposomal si-RNA delivery for treatment of epithelial cancer

et al. 2014

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Background: RNA interference (RNAi) technology using short interfering RNA (si-RNA) has shown immense potential in the treatment of cancers through silencing of specific genes. Cationic non-viral vectors employed for gene delivery exhibit toxic effects in normal cells limiting their widespread use, therefore, site-specific delivery using benign carriers could address this issue. Objective: Design of a non-toxic carrier that enables site-specific delivery of si-RNA into the cancer cells is of prime importance to realize the promise of gene silencing. Methods: In the present study, non-cationic liposomes encapsulating si-RNA against epithelial cell adhesion molecule (EpCAM) were developed and characterized for encapsulation efficiency, colloidal stability, in vitro and in vivo gene silencing efficacy. Results: PEGylated liposomes containing phosphatidyl choline and phosphatidyl ethanolamine exhibited maximum si-RNA encapsulation efficiency of 47%, zeta potential of -21 mV, phase transition temperature of 51 C and good colloidal stability in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and plasma protein (PP) at 37 C. Conjugation of epithelial cell adhesion molecule (EpCAM) antibody to the liposomes resulted in enhanced cell internalization and superior down-regulation of EpCAM gene in MCF-7 cell lines when compared with free si-RNA and the non-targeted liposomes. In vivo evaluation of immunoliposomes for their efficacy in regressing the tumor volume in Balb/c SCID mice showed about 35% reduction of tumor volume in comparison with the positive control when administered with an extremely low dose of 0.15 mg/kg twice a week for 4 weeks. Conclusion: Our results exhibit that the nanocarrier-mediated silencing of EpCAM gene is a promising strategy to treat epithelial cancers.

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“…All test groups were shaken gently and incubated at room temperature for 30 min. 41,48,49 The process was stopped by adding 5 mL of loading buffer. Then, the samples were electrophoresed in 1% (w/v) agarose gel containing ethidium bromide (EtBr, 2 mg mL À1 ) at 120 V for 20 min.…”

Section: Preparation Of Go-pll-sdgr and Go-pll-sdgr/ Vegf-sirnamentioning

confidence: 99%

Functionalized graphene oxide for anti-VEGF siRNA delivery: preparation, characterization and evaluation in vitro and in vivo

et al. 2017

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PEGylated carboxymethyl chitosan/calcium phosphate hybrid anionic nanoparticles mediated hTERT siRNA delivery for anticancer therapy

Cited by 140 publications

References 45 publications

Evaluation of dendrimer type bio-reducible polymer as a siRNA delivery carrier for cancer therapy

Evaluation of dendrimer type bio-reducible polymer as a siRNA delivery carrier for cancer therapy

EpCAM-targeted liposomal si-RNA delivery for treatment of epithelial cancer

Functionalized graphene oxide for anti-VEGF siRNA delivery: preparation, characterization and evaluation in vitro and in vivo

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