PEGylation of alpha-momorcharin: synthesis and characterization of novel anti-tumor conjugates with therapeutic potential

Bian, Xiaoxiao; Shen, Fubing; Chen, Yiwen; Wang, Baoning; Deng, Mian; Yan-fa, Meng

doi:10.1007/s10529-010-0242-8

Cited by 41 publications

(40 citation statements)

References 16 publications

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“…Four months later, the column worked for 30 times, and the result showed that the reusability of the gel is good. As was reported previously, with small scale, about 3.0 mg a-MMC was obtained from 2.5 g bitter melon after two ion exchange chromatographic steps on DEAE cellulose and mono-S FPLC [18], and 350 mg protein was purified from 200 g bitter melon seeds by the strategy of purification with four steps: supernatant extraction, ammonium sulfate fractionation precipitation, MacroCap SP chromatography and Superdex 75 chromatography [12]. Using immunoaffinity chromatography on the anti-a-MMC-McAb-Sepharose 4B, 196 mg a-MMC with 2.7% yield was obtained from 100 g bitter melon.…”

Section: Purification and Characterization Of A-momorcharin On Immunomentioning

confidence: 91%

“…The slurry extract was stirred for overnight; after simple filtration through cheese cloth, the supernatant was fractionated by ammonium sulfate (35-70%). The deposits were dissolved in pH 7.0 50 mM Tris-HCl and dialyzed against the same buffer [12]. After centrifugation, the supernatant was applied to an anti-a-MMC McAb-Sepharose 4B column equilibrated with pH 7.0 50 mL Tris-HCl buffer; the column was sufficiently washed to remove unbound impurities with the pH 7.0 50 mM Tris-HCl buffer.…”

Section: Purification Of A-mmc By Anti-a-mmc Mcabsepharose 4bmentioning

confidence: 99%

“…More interestingly, its strong anti-tumor growth activity and anti-human immunodeficiency virus (HIV) function make it a potential drug candidate [10,11]. To shield its immunogenicity and extend its plasma half-life, we have PEGylated a-MMC without significant decrease in its anti-tumor/viral activities [12].…”

Section: Introductionmentioning

confidence: 99%

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Immunoaffinity purification of α‐momorcharin from bitter melon seeds (Momordica charantia)

Yao

Deng

et al. 2011

J of Separation Science

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α-Momorcharin (α-MMC), a type I ribosome-inactivating protein (RIP), has shown therapeutic potential such as anti-tumor and anti-viral agent. Traditional process of α-MMC purification from bitter melon seeds was time consuming and low efficient. To take this challenge, we made an affinity matrix by coupling the monoclonal antibody (McAb) with Sepharose 4B. Using this attractive strategy, 196 mg of α-MMC was obtained from 100 g of bitter melon seeds as the starting material. The yield of the protein was 2.7%. The homogeneity and properties of the protein were assessed by SDS-PAGE, acidic PAGE, RP-HPLC and N-terminal sequence as well as Western blot. Purified α-MMC showed remarkable inhibition to the melanoma cell line JAR and EMT-62058. In addition, it also displayed obvious inhibition on hepatitis B virus (HBV). This work provided a simple, rapid and efficient approach for α-MMC purification from Momordica charantia.

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Section: Purification and Characterization Of A-momorcharin On Immunomentioning

confidence: 91%

Section: Purification Of A-mmc By Anti-a-mmc Mcabsepharose 4bmentioning

confidence: 99%

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Immunoaffinity purification of α‐momorcharin from bitter melon seeds (Momordica charantia)

Yao

Deng

et al. 2011

J of Separation Science

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“…The α-MMC was extracted from seeds of M. charantia according to the method of Bian et al (2010). JA, SA, ibuprofen, and ABT were purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

The Roles of Alpha-Momorcharin and Jasmonic Acid in Modulating the Response of Momordica charantia to Cucumber Mosaic Virus

et al. 2016

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Alpha-momorcharin (α-MMC) is a type-I ribosome inactivating protein with a molecular weight of 29 kDa that is found in Momordica charantia, and has been shown to be effective against a broad range of human viruses as well as having anti-tumor activities. However, the role of endogenous α-MMC under viral infection and the mechanism of the anti-viral activities of α-MMC in plants are still unknown. To study the effect of α-MMC on plant viral defense and how α-MMC increases plant resistance to virus, the M. charantia–cucumber mosaic virus (CMV) interaction system was investigated. The results showed that the α-MMC level was positively correlated with the resistance of M. charantia to CMV. α-MMC treatment could alleviate photosystem damage and enhance the ratio of glutathione/glutathione disulfide in M. charantia under CMV infection. The relationship of α-MMC and defense related phytohormones, and their roles in plant defense were further investigated. α-MMC treatment led to a significant increase of jasmonic acid (JA) and vice versa, while there was no obvious relevance between salicylic acid and α-MMC. In addition, reactive oxygen species (ROS) were induced in α-MMC-pretreated plants, in a similar way to the ROS burst in JA-pretreated plants. The production of ROS in both ibuprofen (JA inhibitor) and (α-MMC+ibuprofen)-pretreated plants was reduced markedly, leading to a greater susceptibility of M. charantia to CMV. Our results indicate that the anti-viral activities of α-MMC in M. charantia may be accomplished through the JA related signaling pathway.

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“…Alpha-momorcharin (α-MMC), from a ribosome-inactivating protein (RIP) exhibited excellent cytotoxicity against tumor cells and strong immunogenicity and short half-life time in plasma. Bian et al [126] synthesized PEGylated-α-MMC using a branched mPEG. The results indicated that the complex preserved moderate anticancer activity and a longer circulation time with 36% acute toxicity and at most 66% immunogenicity decrease.…”

Section: The Pegylation Of Acpsmentioning

confidence: 99%

Design and Modification of Anticancer Peptides

Hu¹,

Chen²,

Zhao³

et al. 2016

Drug Des

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Cancer is a great concern in the public health and development of novel therapeutic agent or strategy become urgently. Anticancer peptides (ACPs) have become promising anticancer drug candidate molecules due to their several extraordinary properties, such as small size, high activity, low immunogenicity, good biocompatibility, diversity of sequence and more modification sites for the functional molecules. However, the low stability and short half-life of peptides are the major barriers for the application. In this review, we focus on the methods of peptide design and modification to improve the stability, half-life time and specificity of ACPs, which including amino acid substitution, cyclization, hybridization, fragmentization, modification of C-and N-terminal of peptide by polymer or targeting molecules, etc.modification of C-and N-terminal of peptide by polymer or targeting molecules, etc. The schematic diagram of major design and modification strategies of ACPs are shown in Figure 1.

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PEGylation of alpha-momorcharin: synthesis and characterization of novel anti-tumor conjugates with therapeutic potential

Cited by 41 publications

References 16 publications

Immunoaffinity purification of α‐momorcharin from bitter melon seeds (Momordica charantia)

Immunoaffinity purification of α‐momorcharin from bitter melon seeds (Momordica charantia)

The Roles of Alpha-Momorcharin and Jasmonic Acid in Modulating the Response of Momordica charantia to Cucumber Mosaic Virus

Design and Modification of Anticancer Peptides

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