PEGylation of dendronized silver nanoparticles increases the binding affinity of antimicrobial proteins

Ciepluch, Karol; Skrzyniarz, Kinga; Zdańska, Justyna; Barrios-Gumiel, Andrea; Sánchez‐Nieves, Javier; Mata, F. Javier de la; Maciejewska, Barbara; Drulis-Kawa, Zuzanna; Bryszewska, Maria; Arabski, Michał

doi:10.1016/j.molliq.2020.114339

Cited by 14 publications

(8 citation statements)

References 41 publications

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“…34 Interestingly, it was also shown that endolysin and lysozyme have a higher affinity for PEGylated dendronized AgNPs, while HSA exhibits higher affinity for non-PEGylated variants. 45 Considering the interactions of PEGylated gold nanoparticles with siRNA and comparing them with previous analyses of non-PEGylated AuNPs, 10 as expected, a decrease in the interactions was observed with increasing PEG concentration. This was especially visible for AuNP13 analogues, where only AuNP13a (the least modified with PEG) retained a low siRNA binding capacity, observed both by ζ-potential studies and gel electrophoresis.…”

Section: ■ Discussionsupporting

confidence: 57%

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PEGylation of Dendronized Gold Nanoparticles Affects Their Interaction with Thrombin and siRNA

et al. 2021

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The use of nonviral carriers based on nanomaterials is a promising strategy for modern gene therapy aimed at protecting the genetic material against degradation and enabling its efficient cellular uptake. To improve the effectiveness of nanocarriers in vivo, they are often modified with poly(ethylene glycol) (PEG) to reduce their toxicity, limit nonspecific binding by proteins in the bloodstream, and extend blood half-life. Thus, the selection of an appropriate degree of surface PEGylation is crucial to preserve the interaction of nanoparticles with the genetic material and to ensure its efficient transport to the site of action. Our research focuses on the use of innovative gold nanoparticles (AuNPs) coated with cationic carbosilane dendrons as carriers of siRNA. In this study, using dynamic light scattering and zeta potential measurements, circular dichroism, and gel electrophoresis, we investigated dendronized AuNPs modified to varying degrees with PEG in terms of their interactions with siRNA and thrombin to select the most promising PEGylated carrier for further research.

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Section: ■ Discussionsupporting

confidence: 57%

“…In both cases, non-PEGylated nanoparticles had a stronger effect . Interestingly, it was also shown that endolysin and lysozyme have a higher affinity for PEGylated dendronized AgNPs, while HSA exhibits higher affinity for non-PEGylated variants …”

Section: Discussionmentioning

confidence: 94%

PEGylation of Dendronized Gold Nanoparticles Affects Their Interaction with Thrombin and siRNA

et al. 2021

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“…However, with some single exceptions of endolysins reviewed by Gerstmans et al that permeate the OM barrier per se, the exogenous application of native phage endolysins on Gram-negatives is rather ineffective [28]. Nevertheless, our previous studies on cationic dendrimers with different skeleton nature showed their positive impact on the improvement of endolysin antibacterial activity [22,26,29]. In this work, KP27 endolysin with the activity of L-alanyl-D-glutamate endopeptidase was mixed with the CBS dendrimer functionalized with ammonium groups (NMe 3 + ) alone (4) or with PEG (PEG800 (7), PEG2K (10)) to verify a possible synergic effect (Figure 5).…”

Section: Antibacterial Activity Of Dendrimer Combined With Endolysin ...mentioning

confidence: 97%

The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin

Quintana-Sánchez

Gómez-Casanova

Sánchez‐Nieves

et al. 2022

IJMS

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The search for new microbicide compounds is of an urgent need, especially against difficult-to-eradicate biofilm-forming bacteria. One attractive option is the application of cationic multivalent dendrimers as antibacterials and also as carriers of active molecules. These compounds require an adequate hydrophilic/hydrophobic structural balance to maximize the effect. Herein, we evaluated the antimicrobial activity of cationic carbosilane (CBS) dendrimers unmodified or modified with polyethylene glycol (PEG) units, against planktonic and biofilm-forming P. aeruginosa culture. Our study revealed that the presence of PEG destabilized the hydrophilic/hydrophobic balance but reduced the antibacterial activity measured by microbiological cultivation methods, laser interferometry and fluorescence microscopy. On the other hand, the activity can be improved by the combination of the CBS dendrimers with endolysin, a bacteriophage-encoded peptidoglycan hydrolase. This enzyme applied in the absence of the cationic CBS dendrimers is ineffective against Gram-negative bacteria because of the protective outer membrane shield. However, the endolysin—CBS dendrimer mixture enables the penetration through the membrane and then deterioration of the peptidoglycan layer, providing a synergic antimicrobial effect.

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“…In a new approach for the usage of AgNPs to avoid the development of resistance by pathogens, researchers found that it is time to get another line of treatment. In this scenario, the conjugation of AgNPs and dendron can be a promising strategy to combat antibiotic-resistant pathogens, thanks to dendrons’ antimicrobial activity and capacity to deliver antibacterial agents such as antimicrobial proteins (AMP), lysozyme, and bacteriophage endolysin [ 235 ]. Different antibiotics and AMPs bind to the dendrimers to improve the potency of the drug molecules against different MDR strains.…”

Section: Silver Nanoparticles and Antibacterial Activitymentioning

confidence: 99%

Silver Nanoparticles: Bactericidal and Mechanistic Approach against Drug Resistant Pathogens

et al. 2023

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This review highlights the different modes of synthesizing silver nanoparticles (AgNPs) from their elemental state to particle format and their mechanism of action against multidrug-resistant and biofilm-forming bacterial pathogens. Various studies have demonstrated that the AgNPs cause oxidative stress, protein dysfunction, membrane disruption, and DNA damage in bacteria, ultimately leading to bacterial death. AgNPs have also been found to alter the adhesion of bacterial cells to prevent biofilm formation. The benefits of using AgNPs in medicine are, to some extent, counter-weighted by their toxic effect on humans and the environment. In this review, we have compiled recent studies demonstrating the antibacterial activity of AgNPs, and we are discussing the known mechanisms of action of AgNPs against bacterial pathogens. Ongoing clinical trials involving AgNPs are briefly presented. A particular focus is placed on the mechanism of interaction of AgNPs with bacterial biofilms, which are a significant pathogenicity determinant. A brief overview of the use of AgNPs in other medical applications (e.g., diagnostics, promotion of wound healing) and the non-medical sectors is presented. Finally, current drawbacks and limitations of AgNPs use in medicine are discussed, and perspectives for the improved future use of functionalized AgNPs in medical applications are presented.

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PEGylation of dendronized silver nanoparticles increases the binding affinity of antimicrobial proteins

Cited by 14 publications

References 41 publications

PEGylation of Dendronized Gold Nanoparticles Affects Their Interaction with Thrombin and siRNA

PEGylation of Dendronized Gold Nanoparticles Affects Their Interaction with Thrombin and siRNA

The Antibacterial Effect of PEGylated Carbosilane Dendrimers on P. aeruginosa Alone and in Combination with Phage-Derived Endolysin

Silver Nanoparticles: Bactericidal and Mechanistic Approach against Drug Resistant Pathogens

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