PEGylation of αα-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affinity and resuscitation fluid like properties of PEG αα-Hbs

Meng, Fantao; Tsai, Amy G.; Intaglietta, Marcos; Acharya, Seetharama A.

doi:10.3109/21691401.2014.885443

Cited by 4 publications

(1 citation statement)

References 44 publications

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“…Consequently, red blood cell (RBC) substitutes for practical use are required as a medical measure to supplement blood transfusion treatment. Since the 1980s, hemoglobin (Hb)-based O 2 carriers (HBOCs) of several kinds have been prepared and clinically tested [3][4][5][6][7][8][9][10][11][12]. Despite great efforts, none has been approved yet for commercial use as an RBC alternate.…”

Section: Introductionmentioning

confidence: 99%

Hemoglobin–albumin cluster: physiological responses after exchange transfusion into rats and blood circulation persistence in dogs

Iwasaki

Yokomaku

Kureishi

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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A core-shell protein cluster comprising hemoglobin and human serum albumins, hemoglobin-albumin cluster (Hb-HSA 3), was designed and synthesized for use as an artificial O 2 carrier and red blood cell (RBC) substitute. For initial preclinical safety evaluation of the Hb-HSA 3 solution, we observed blood compatibility in vitro, physiological responses after exchange transfusion into rats and blood circulation lifetime in dogs. Dilution of human whole blood with Hb-HSA 3 showed an appropriate decrease in blood cell number, proportional to the mixing volume ratio. Time courses in the circulation parameters and blood gas parameters after 20% exchange transfusion with Hb-HSA 3 in anesthetized rats were almost identical to those observed in a sham group (without infusion) and an HSA group (with HSA administration) for 6 h. Serum biochemical tests of the withdrawn blood indicated safety of the protein cluster. Furthermore, fluorescent Hb-HSA 3 was infused into beagle dogs to assess blood retention. Fluorescence measurements of the blood samples enabled us to ascertain the cluster half-life within the intravascular space. Histopathologic inspections of the vital organs imply no abnormality in tissues. All these results indicate sufficient initial preclinical safety of Hb-HSA 3 as an alternative material for use in RBC transfusion.

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Section: Introductionmentioning

confidence: 99%

Hemoglobin–albumin cluster: physiological responses after exchange transfusion into rats and blood circulation persistence in dogs

Iwasaki

Yokomaku

Kureishi

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Numerical investigation of oxygen transport by hemoglobin-based carriers through microvessels

Hyakutake

Kishimoto

2017

J Artif Organs

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The small size of hemoglobin-based oxygen carriers (HBOCs) may expand the realm of new treatment possibilities for various circulatory diseases. The parametric evaluation of HBOC performance for oxygen transport within tissue is essential for effectively characterizing its performance for each circulatory disease assessed. Thus, the overarching objective of this present study was to numerically investigate the reaction-diffusion phenomenon of oxygenated HBOCs and oxygen on tissues through microvessels. We considered dissociation rate coefficients, oxygen affinity, and diffusion coefficients due to Brownian motion as the biophysical parameters for estimating HBOC performance for oxygen transport. A two-dimensional computational domain, including vessel and tissue regions, was, therefore, accordingly assumed. It was observed that HBOC flows in a microvessel with a diameter of 25 μm and a length of 1 mm, and that the dissociated oxygen diffuses to the tissue region. The results indicated that oxyhemoglobin saturation and partial oxygen tension in a downstream region changed according to each biophysical parameter of HBOC. Moreover, the change in oxygen consumption rate in the tissue region had considerable influence on the oxyhemoglobin saturation level within the vessel. Comparison between simulation results and existing in vitro experimental data of actual HBOCs and RBC showed qualitatively good agreement. These results provide important information for the effective design of robust HBOCs in future.

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Development of Hemoglobin Microbubbles for Acoustic Blood Oxygen Sensing: A Study on PEGylation and Gas Core Modification for In vivo Applications

Rastegar,

Kianpour,

Pathour

et al. 2024

Acta Biomaterialia

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PEGylation of αα-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affinity and resuscitation fluid like properties of PEG αα-Hbs

Cited by 4 publications

References 44 publications

Hemoglobin–albumin cluster: physiological responses after exchange transfusion into rats and blood circulation persistence in dogs

Hemoglobin–albumin cluster: physiological responses after exchange transfusion into rats and blood circulation persistence in dogs

Numerical investigation of oxygen transport by hemoglobin-based carriers through microvessels

Development of Hemoglobin Microbubbles for Acoustic Blood Oxygen Sensing: A Study on PEGylation and Gas Core Modification for In vivo Applications

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