Peiminine inhibits the IL-1β induced inflammatory response in mouse articular chondrocytes and ameliorates murine osteoarthritis

Luo, Zucheng; Zheng-Lin, Binbin; Jiang, Bingjie; Xue, Xinghe; Xue, Enxing; Zhou, Yulong

doi:10.1039/c9fo00307j

Cited by 36 publications

(27 citation statements)

References 34 publications

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“…In another report, sipeimine and peiminine from bulbs of F. wabuensis inhibited pro-inflammatory mediators in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cells [ 15 ]. More recently, it was demonstrated that peimine relieved inflammatory effects in IL-1β-induced chondrocytes, indicating that peimine might be a potential therapeutic agent for osteoarthritis [ 16 ]. Antitussive, expectorant, and anti-inflammatory effects of several alkaloids, including sipeimine, chuanbeinone, peiminine, and peimine isolated from Bulbus Fritillaria cirrhosa were demonstrated in mice by using a phytochemical method [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

Chen

Lee

et al. 2020

Plants

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Investigations were carried out to study the effects of light-emitting diode (LED) lights on growth and development of isosteroidal alkaloids in embryogenic calli of Fritillaria cirrhosa D. Don, an important traditional Chinese medicine herb. Calli were cultured in glass bottles, each containing 100 mL of Murashige and Skoog’s basal medium supplemented with 2% sucrose and 0.4% gellan gum powder, a gelling agent. These bottles were incubated in a specially designed plant growth chamber equipped with eight different LED lights consisting of single or combinations of four different light spectra emitting blue (450 nm), green (525 nm), red (660 nm), and far-red (730 nm) light. After three months of incubation, morphological changes in embryogenic calli were recorded, and LC-MS/MS analysis of cultures was carried out for peimisine, sipeimine, peiminine, and peimine. The highest number of somatic embryos and the maximum fresh weight was recorded in calli incubated under red (9R), infrared (9IR), and a combination of red+blue+infrared (3R3B3IR), respectively, in decreasing order. The highest contents of peimisine, peiminine, and peimine were recorded under red (9R) and infrared (9IR) lights, respectively. Eight LED lights had significant effects on the morphogenesis of embryogenic calli of F. cirrhosa D. Don and contents of isosteroidal alkaloids.

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Section: Introductionmentioning

confidence: 99%

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

Chen

Lee

et al. 2020

Plants

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“…Damage of articular cartilage caused the changes in extracellular matrix of cartilage 3. Collagen II and aggrecan are the primary composition of the extracellular matrix in cartilage, and matrix metalloproteinase (MMPs) are very important for maintaining integrity of cartilage 4. Inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and IL-1β were considered to be important in inducing damage of articular cartilage 5,6.…”

Section: Introductionmentioning

confidence: 99%

“…Inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and IL-1β were considered to be important in inducing damage of articular cartilage 5,6. IL-1β could regulate the differentiation and function of chondrocytes, which promotes the expression of MMPs; IL-1β then breaks down the cartilage matrix via inducing cell apoptosis 4,7. Therefore, inhibiting IL-1β-induced inflammation might be an effective therapeutic strategy for treating OA.…”

Section: Introductionmentioning

confidence: 99%

<p>Overexpression of SOX9 alleviates the progression of human osteoarthritis in vitro and in vivo</p>

Ouyang¹,

Wang²,

Tu³

et al. 2019

DDDT

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Purpose Recent findings have identified that SOX9 served as a key role during the pathogenesis of osteoarthritis (OA). This study aimed to investigate the mechanisms by which SOX9 regulated the formation of OA in vitro and in vivo. Materials and methods The relative expressions of SOX9 in patients with OA and normal fracture of thighbone were analyzed by real-time-PCR. In vitro, IL-1β induced inflammatory response in human chondrocytes was used to evaluate the function of SOX9. The recombinant SOX9 lentivirus vector (Lenti-SOX9) was used to upregulate the expression of SOX9 in cells. ELISA was used to measure the concentration of tumor necrosis factor-α (TNF-α). The protein expressions of SOX9, matrix metalloproteinase-13 (MMP13), Collagen II, Aggrecan and Smad3 were analyzed by Western blot. Cell proliferation and cell apoptosis were detected by CCK-8 assay and flow cytometry, respectively. In vivo, the effect of SOX9 on surgically induced OA mice was evaluated. Results The gene level of SOX9 was remarkably downregulated in patients with OA compared with normal people, while the concentration of TNF-α was upregulated. In addition, IL-1β reduced the expressions of SOX9, Collagen II and Aggrecan and increased the level of MMP13 in chondrocytes. Moreover, Lenti-SOX9 notably inhibited IL-1β-induced growth inhibition and apoptosis in chondrocytes via increasing the expression of Smad3. Finally, Lenti-SOX9 markedly alleviated the symptoms of OA mice in vivo. Conclusion Upregulation of SOX9 inhibited IL-1β-induced inflammatory response via increasing the level Smad3 in human chondrocytes and exhibited therapeutic effect on surgically induced OA mice in vivo. Therefore, SOX9 may serve as a potential target in the treatment of OA in the future.

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“…In the process of the development of OA, abnormal metabolic dysfunction occurs in chondrocytes, and the synthesis of aggrecan and collagen II, the main components of chondrocyte extracellular matrix, are inhibited, while the synthesis of MMPs is increased, thus promoting the abnormal degradation of chondrocyte extracellular matrix (30)(31)(32). In addition, the large release of inflammatory factors such as IL-1β, IL-6, NO, PGE2 and TNF-α not only damages chondrocytes, but also induces extracellular matrix degradation, which is also an important reason for promoting the development of OA (33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

LINC01534 Promotes the Aberrant Metabolic Dysfunction and Inflammation in IL-1β-Simulated Osteoarthritic Chondrocytes by Targeting miR-140-5p

Wei

Wang

et al. 2019

CARTILAGE

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Objective Long non-coding RNA 01534 (LINC01534) is highly expressed in the tissues of patients with osteoarthritis (OA). This study investigated the mechanism of LINC01534 on abnormal metabolic dysfunction in OA chondrocytes induced by interleukin-1β (IL-1β). Methods The quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expressions of LINC01534, aggrecan, collagen II, and matrix metalloproteinase (MMPs) in OA cartilage tissue or OA chondrocyte model induced by IL-1β. The expressions of aggrecan and collagen II in the chondrocyte were detected by Western blot. The levels of tumor necrosis factor–α (TNF-α), IL-8, IL-6, MMP-13, MMP-9, MMP-3, and prostaglandin E2 (PGE2) in chondrocyte were determined by enzyme-linked immunosorbernt assay. Bioinformatics, dual luciferin gene reporting, RNA pulldown, and Northern blot were used to determine the interaction between LINC01534 and miR-140-5p. Results The results showed that LINC01534 was upregulated in both OA cartilage tissue and OA chondrocyte model. In addition, silencing LINC01534 significantly alleviated the inhibitory effect of IL-1β on expressions of aggrecan and collagen II in chondrocytes, and significantly downregulated the expression of matrix metalloproteinases in IL-1β-induced chondrocytes. Meanwhile, silencing LINC01534 also significantly inhibited the productions of proinflammatory factors NO, PGE2, TNF-α, IL-6, and IL-8 in the IL-1β-induced chondrocytes. Furthermore, miR-140-5p was confirmed to be a direct target of LINC01534. More importantly, inhibition of miR-140-5p significantly reversed the inhibitory effect of silencing LINC01534 on abnormal matrix degradation in the IL-1β-induced chondrocyte model of OA. Conclusion Therefore, LINC01534 could promote the abnormal matrix degradation and inflammatory response of OA chondrocytes through the targeted binding of miR-140-5p.

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Peiminine inhibits the IL-1β induced inflammatory response in mouse articular chondrocytes and ameliorates murine osteoarthritis

Abstract: Osteoarthritis (OA) is a common arthrosis characterized by degeneration and inflammation of articular cartilage.

Cited by 36 publications

References 34 publications

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

LED Lights Affecting Morphogenesis and Isosteroidal Alkaloid Contents in Fritillaria cirrhosa D. Don—An Important Chinese Medicinal Herb

<p>Overexpression of SOX9 alleviates the progression of human osteoarthritis in vitro and in vivo</p>

LINC01534 Promotes the Aberrant Metabolic Dysfunction and Inflammation in IL-1β-Simulated Osteoarthritic Chondrocytes by Targeting miR-140-5p

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