PELDOR Measurements on Nitroxide-Labeled Oligonucleotides

Abstract

Most results presented in this thesis are the outcome of teamwork, and I would like to thank all my collaborators for their valuable contributions. In the project on time-resolved PELDOR, I had the pleasure to collaborate with Prof. Dr. U. Benjamin Kaupp and his group at the Max Planck Institute for Neurobiology of Behavior -caesar, Dr. Reinhard Seifert, Dr. Tobias Zbik, and Norbert Brenner. Furthermore, I would like to thank Prof. Dr. Helmut Grubmüller and Dr. Reinhard Klement from the Max Planck Institute for Multidisciplinary Sciences in Göttingen for introducing me to the field of molecular dynamics simulations and their helpful advice.The EPR measurements on trityls were done in collaboration with Dr. Nico Fleck, Jean-Jacques Jassoy, and Caspar Heubach and I would like to thank them for synthesizing the trityl spin labels and for labelling the proteins. Special thanks go to Dr. Dinar Abdullin for his advice on spectrometer operation and our enlightening discussions on various topics such as spin physics, programming, and data fitting. Likewise, I would like to thank Hamed Alaei for his technical support and for his cooperation in conducting the students' practical courses. Beyond, I would like to express my gratitude to all present and previous members of the research group for the nice working atmosphere and for fruitful discussions -especially my office mates, Catrin Allar, Olivia Kendall, Kevin Kopp, and Maria Vicino, shall be mentioned here.The computation centre of the University of Bonn is acknowledged for granting access to the Bonna cluster and the Deutsche Forschungsgemeinschaft (DFG, project number 420322655, Koselleck grant to U. B. Kaupp) for funding my research.Finally, I would like to thank my friends and family, especially my parents and my brother, for supporting me during my studies.