Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production

Supramani, Sugenendran; Jailani, Nurulhuda Mohd; Ramarao, Kivaandra Dayaa Rao; Zain, Nurul Amalina Mohd; Klaus, Anita; Ahmad, Rahayu; Wan‐Mohtar, Wan Abd Al Qadr Imad

doi:10.1016/j.bcab.2019.101118

Cited by 30 publications

(25 citation statements)

References 29 publications

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“…The highest biomass (9.51 ± 0.2 g/L) was produced in cycle R7 with pellet diameter (49.00 ± 0.82< d <51.33 ± 1.25) µm, which was recorded to be most significant for biomass production in BRR 75%, BRTP at day 11. The compact pellet structure gave high biomass production, which was in agreement with the work done using Ganoderma pfeifferi by Supramani et al, 2019a [4] .…”

Section: Resultssupporting

confidence: 90%

“…Formation of starburst-like pellet indicates a new cycle of culture, along with continuous replacement of old media with the new one at R6, and the small-compact pellets were formed at R7. Formation of hairy like structure around the pellet during the seven cycles of RBF indicated the continuous growth of fungal pellet and the new environment created by the replacement of media made the growth of the culture varied [4] .…”

Section: Resultsmentioning

confidence: 99%

“…A further study conducted by Wan-Mohtar et al, 2016 [10] proved that the production of polysaccharide is directly affected by the morphology of mycelial pellets. Small-loosely branched mycelium pellets produce higher polysaccharide compared to large mycelium pellets [4] . In this study, the ideal pellet diameters of GL strain QRS 5120 were reported for the highest total biomass, exopolysaccharide and endopolysaccharide productions, as well as its anti-oral β-glucan (BG) bioactive properties.…”

Section: Introductionmentioning

confidence: 95%

“…SLF caught researchers' attention due to its high yield of biomass and polysaccharide production in a shorter time period and with lower costs compared to SSF [3] . There are many ways to perform SLF, but the most promising production of a bioactivity compound from mushroom has been seen in repeated-batch fermentation (RBF) [4] . RBF is an adjustment of batch fermentation, in which a specific amount of medium is extracted and replaced by the same amount of medium extracted with a new medium, either intermittently, or more than once without changing the culture.…”

Section: Introductionmentioning

confidence: 99%

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Pellet diameter of <em>Ganoderma lucidum</em> in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan response

et al. 2020

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The pellet morphology and diameter range (DR) of Ganoderma lucidum were observed in a repeated-batch fermentation (RBF) for the trio total production of biomass, exopolysaccharide (EPS) and endopolysaccharide (ENS). Two factors were involved in RBF; broth replacement ratio (BRR: 60%, 75% and 90%) and broth replacement time point (BRTP: log, transition and stationary phase) in days. In RBF, 34.31 g/L of biomass favoured small-compact pellets with DR of 20.67 µm< d < 24.00 µm (75% BRR, day 11 of BRTP). EPS production of 4.34 g/L was prone to ovoid-starburst pellets with DR of 34.33 µm< d <35.67 µm (75% BRR, day 13 of BRTP). Meanwhile, the highest 2.43 g/L of ENS production favoured large-hollow pellets with DR of 34.00 µm< d < 38.67 µm (90% BRR, day 13 of BRTP). In addition, RBF successfully shortened the biomass-EPS–ENS fermentation period (31, 33 and 35 days) from batch to 5 days, in seven consecutive cycles of RBF. In a FTIR detection, β-glucan (BG) from EPS and ENS extracts were associated with β-glycosidic linkages (2925 cm −1 , 1635 cm −1 , 1077 cm −1 , 920 cm −1 and 800 cm −1 wavelengths) with similar 1 H NMR spectral behaviour (4.58, 3.87 and 3.81 ppm). Meanwhile, 4 mg/L of BG gave negative cytotoxic effects on normal gingival cell line (hGF) but induced antiproliferation (IC 50 = 0.23 mg/mL) against cancerous oral Asian cellosaurus cell line (ORL-48). Together, this study proved that G. lucidum mycelial pellets could withstand seven cycles of long fermentation condition and possessed anti-oral cancer beta-glucan, which suits large-scale natural drug fermentation.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pellet diameter of <em>Ganoderma lucidum</em> in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan response

et al. 2020

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“…(2019) and Supramani et al. (2019). The jellified extract was concentrated under vacuum at 40 °C using a rotary evaporator and stored at 4 °C.…”

Section: Methodsmentioning

confidence: 95%

Fruiting‐body‐base flour from an Oyster mushroom waste in the development of antioxidative chicken patty

Wan‐Mohtar

Lim

Kamarudin

et al. 2020

Journal of Food Science

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In a commercial oyster mushroom farm, from 300 g of the total harvest, only the cap and stem of the fruiting body parts are harvested (200 g) while the unused lower section called fruiting-body-base (FBB) is discarded (50 g). A new antioxidative FBB flour (FBBF) conversion to mixed-ratio chicken patty was recently developed which converts 16.67% of FBB into an edible flour. At the initial stage, pretreatments of FBBF were optimized at particle size (106 μm) and citric acid concentration (0.5 g/100 mL) to improve flour antioxidant responses. Such pretreatments boosted total phenolic content (2.31 ± 0.53 mg GAE/g) and DPPH (51.53 ± 1.51%) of pretreated FBBF. Mixed-ratio chicken patty containing FBBF (10%, 20%, 30%) significantly (P < 0.05) influenced the hardness, cohesiveness, springiness, and chewiness of the patties. However, only the hardness and chewiness increased proportionally with the increase FBBF in concentration. Notably, 60 panellists considered that 10% FBBF-chicken patty sensory attributes, including lightness, redness, and yellowness, is acceptable to consumers. This information could be used to market any type of commercial mushroom farm waste as alternative food products.

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Optimization of mycoremediation treatment for the chemical oxygen demand (COD) and ammonia nitrogen (AN) removal from domestic effluent using wild-Serbian Ganoderma lucidum (WSGL)

Mooralitharan

Hanafiah

Manan

et al. 2021

Environ Sci Pollut Res

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Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production

Cited by 30 publications

References 29 publications

Pellet diameter of <em>Ganoderma lucidum</em> in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan response

Pellet diameter of <em>Ganoderma lucidum</em> in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan response

Fruiting‐body‐base flour from an Oyster mushroom waste in the development of antioxidative chicken patty

Optimization of mycoremediation treatment for the chemical oxygen demand (COD) and ammonia nitrogen (AN) removal from domestic effluent using wild-Serbian Ganoderma lucidum (WSGL)

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