Pemanfaatan plasma nutfah padi (Oryza sativa) untuk perbaikan sifat padi gogo

Hairmansis, Aris; Supartopo, Supartopo; Yullianida, Yullianida; Sunaryo, Sunaryo; Warsono, Warsono; Sukirman, Sukirman; Suwarno, Suwarno

doi:10.13057/psnmbi/m010102

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…Perakitan varietas padi dengan menggunakan dan memanfaatkan keunggulan spesifik yang dimiliki varietas lokal diharapkan dapat meningkatkan keunggulan varietas padi yang dibudidayakan pada spesifik lokasi. Varietas padi lokal Klemas, Gampai, Cenggong, dan Grogol telah digunakan sebagai donor tetua untuk mendapatkan varietas padi tahan blas (Hairmansis et al, 2015). Hasil penelitian menunjukkan adanya keragaman ketahanan yang berbeda-beda pada varietas padi lokal terhadap penyakit blas daun (Nasution & Usyati, 2015).…”

Section: Abstrakunclassified

KERAGAMAN DAN SUMBER GEN KETAHANAN VARIETAS PADI LOKAL TERHADAP PATOGEN Pyricularia grisea PENYEBAB PENYAKIT BLAS

Santoso

Nasution

Yunani

2020

JIPI

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[DIVERSITY AND THE SOURCE OF RESISTANCE GENE OF LOCAL RICE VARIETIES ON THE PATHOGENIC OF Pyricularia grisea CAUSE OF BLAST DISEASE]. Local rice varieties are known to have resistance or source of genes to pests even though their productivity yield is low. The pathogen of Pyricularia grisea is a cause of blast disease, which is one of the obstacles in rice production. The research aims to characterize the resistance of local rice varieties to the pathogen of P. grisea and to evaluate the virulence level of P. grisea pathogens against local rice varieties. A total of 100 local rice varieties and check varieties are susceptible and resistant namely Kencana Bali and Situ Patenggang tested their resistance to 4 dominant pathogenic of P. grisea i.e. races 033, 073, 133 and 173. Inoculation was carried out on stages 4-5 leaves or 18-21 days after seedling in a green house. The results showed a high genetic diversity of local rice varieties against pathogenic races 033, 073, 133 and 173. Based on the response of local rice varieties resistance i.e. moderately resistant (MR), resistant (R) and susceptible (S) to pathogenic races 033, 073 , 133 and 173 obtained 45 resistance response patterns. Cere Bereum varieties which are local rice varieties from West Java and Situ Patenggang resistant check varieties have a resistant response to 4 P. grisea pathogenic races used. A number of local rice varieties also show a resistant and moderately resistant response to the four pathogenic races used include Siam 11, Pare Siriendah, Menyan, Cere Manggu and Enud-Rawa Bogo. Local rice varieties Djedah and Padi Hitam (2) are local rice varieties that have a specific response of resistant or moderately resistant to race 173. Race 133 and 173 have higher virulence rates than those of races 033 and 073 on local rice varieties. The results of this study indicate that there is a great potential for the utilization of local rice varieties, as a source of resistance genes for blast disease for the assembly of rice varieties that are resistant to blast disease.

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Section: Abstrakunclassified

KERAGAMAN DAN SUMBER GEN KETAHANAN VARIETAS PADI LOKAL TERHADAP PATOGEN Pyricularia grisea PENYEBAB PENYAKIT BLAS

Santoso

Nasution

Yunani

2020

JIPI

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“…Pemanfaatan padi lokal yang telah terkarakterisasi dan dievaluasi perlu lebih ditingkatkan dan diintegrasikan dengan program pemuliaan (Silitonga, 2004;Sumarno dan Zuraida, 2008). Di antara beberapa sifat penting dalam program pemuliaan padi, sifat tahan terhadap hama dan penyakit tanaman (HPT) dimiliki oleh banyak padi lokal dan padi liar (Daradjat et al, 2009;Hairmansis et al, 2015). Tercatat sebanyak 456 aksesi padi lokal asal Indonesia yang telah dipublikasikan memiliki gen ketahanan terhadap HPT (Sitaresmi et al, 2013).…”

Section: Pendahuluanunclassified

Kekerabatan Beberapa Aksesi Padi Lokal Tahan Hama Penyakit Berdasarkan Analisis Polimorfisme Marka SSR

Susanto¹,

Satoto²

2018

J. AgroBiogen

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The level of phylogeny among local rice that has identified the resistances to plant pests and diseases needs to be studied to determine the genetic distance, so that information can be used for a recommendation parental crosses. Simple sequence repeat (SSR) markers could be used as one tool to know the degree of phylogeny and the genetic distance between genotypes. The aim of this study was to determine phylogenetic tree and the coefficient of genetic distance of several local rice resistant to host and pest based polymorphic analysis of SSR markers. Fifteen host and pest resistant local rice and one popular variety (Ciherang) were used. Molecular analysis was carried out using eighteen SSR markers. The banding patterns of DNA amplification resulted from PCR on polyacrylamide electrophoresis gel was documented by using UV Transilluminator. The phylogenetic tree showed three clearly separated clusters. Cluster 1 consisted of indica accessions resistant to blast and BLB: Gadis Langsat, Kebo, Bandang Si Gadis, Ciherang, Jawa Wangi Sleman, Marahmay, Takong, Ampek Panjang, Benoraja, and Siawak. Cluster 2 consisted of javanica and indica accessions resistant to BPH, tungro, blast, and BLB: Ase Balucung, Ase Bukne, Pare Lottong, Pare Pulu, and Jadul. Cluster 3 consisted of japonica accession resistant to blast: Kapas. The farthest genetic distance was between Pare Pulu and Ampek Panjang (DA = 0.816). The closest genetic distance was between Pare Pulu dan Pare Lottong (DA = 0.098). This research data can be used as a reference in determining the crossing parents that will be used in plant breeding program for varieties improvement of Indonesian local rice.Keywords: Genetic distance, local rice, kinship, SSR markers. ABSTRAKTingkat kekerabatan antarpadi lokal dengan keunggulan tahan terhadap hama dan penyakit tanaman (HPT) perlu dipelajari untuk mengetahui seberapa jauh kekerabatannya, sehingga informasi yang diperoleh dapat digunakan untuk kebutuhan pemilihan tetua persilangan. Marka simple sequence repeat (SSR) dapat dijadikan salah satu alat bantu untuk mengetahui tingkat kekerabatan antargenotipe. Penelitian ini bertujuan mengetahui tingkat kekerabatan dan jarak genetik beberapa aksesi padi lokal unggul tahan HPT berdasarkan analisis polimorfisme marka SSR. Materi genetik yang digunakan adalah lima belas padi lokal tahan HPT dan varietas Ciherang. Analisis molekuler dilakukan menggunakan delapan belas marka SSR. Pola pita DNA hasil amplifikasi PCR pada elektroforesis gel poliakrilamida didokumentasi dengan visualisasi gel doc/UV transilluminator. Pohon kekerabatan menunjukkan tiga klaster yang terpisah jelas. Klaster 1 terdiri atas aksesi-aksesi subspesies indica tahan blas dan HDB, yaitu Gadis Langsat, Kebo, Bandang Si Gadis, Ciherang, Jawa Wangi Sleman, Marahmay, Takong, Ampek Panjang, Benoraja, dan Siawak. Klaster 2 terdiri atas aksesi-aksesi subspesies javanica dan japonica tahan WBC, tungro, blas, dan HDB, yaitu Ase Balucung, Ase Bukne, Pare Lottong, Pare Pulu, dan Jadul. Klaster 3 berisi aksesi subsp...

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“…Most of Indonesian traditional rice varieties belong to tropical japonica (known as javanica) subspecies which commonly tall stature that make them susceptible to lodge, late maturity, low productive tiller number, deep rooting system, wide leaves, photoperiod insensitivity, long panicles, round seed shape with long awn at the end of the grain, intermediates amylose content, and low yield potential (Sitaresmi et al, 2013;Dwiningsih et al, 2019;Hairmansis et al, 2015;Irawan and Purbayanti, 2008;Siwi and Harahap, 1978). Generally, the traditional rice varieties cultivated in unfavorable field conditions with no inputs of chemical fertilizers and pesticides, such as acidic dry land, high salinity of swampy land, dry land in hilly areas, and flooded area.…”

Section: Introductionmentioning

confidence: 99%

Rojolele: A Premium Aromatic Rice Variety in Indonesia

Dwiningsih¹,

Al-Kahtani²

2022

IJAGST

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Rojolele is an Indonesian traditional rice variety from Klaten (Central Java) that classified as tropical japonica and low land rice which become a premium rice variety and have higher price in the market due to a delicious taste and a fragrance aroma. The agronomic characteristics of Rojolele are having high plant stature with sturdy stems and upright plant shape, thick and rough leaves, strong and deeper root systems, long panicle length, and long duration of life. Rojolele rice cultivation is facing important challenges from drought, diseases, long duration, and lodging. Thus, breeding Rojolele cultivars with resistance to drought, diseases, lodging, and short duration is the major focus for Rojolele rice improvement. Indonesian farmer have learned to successfully cultivate Rojolele by applying practical skills, leading to increase the rice productivity. Molecular breeding program, including Quantitative Trait Loci (QTL), Genome-Wide Association Study (GWAS), genomic selection, and genome editing can be applied to improve Rojolele characteristics. In this review, important agronomic and quality traits, intensification system for irrigation and pest control, mutation breeding, transgenic lines, and also future perspectives for Rojolele research were presented. Rojolele is useful for rice breeding program in order to guarantee the food security to overcome increasing population and climate change.

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Pemanfaatan plasma nutfah padi (Oryza sativa) untuk perbaikan sifat padi gogo

Cited by 3 publications

References 6 publications

KERAGAMAN DAN SUMBER GEN KETAHANAN VARIETAS PADI LOKAL TERHADAP PATOGEN Pyricularia grisea PENYEBAB PENYAKIT BLAS

KERAGAMAN DAN SUMBER GEN KETAHANAN VARIETAS PADI LOKAL TERHADAP PATOGEN Pyricularia grisea PENYEBAB PENYAKIT BLAS

Kekerabatan Beberapa Aksesi Padi Lokal Tahan Hama Penyakit Berdasarkan Analisis Polimorfisme Marka SSR

Rojolele: A Premium Aromatic Rice Variety in Indonesia

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