Pembuatan, Karakterisasi dan Uji In Vitro Nanopartikel Emas Berbasis Konjugat Gom Arab-Vinkristin

Abstract

Gold nanoparticles (AuNP) are potentially developed as nanomedicine because AuNP is easily synthesized, functionalized, and biocompatible. With gum arabic as a stabilizer, vincristine was conjugated with gold nanoparticles. As a reducing agent, it used 0.02 M Natrium Boro Hidrat (NaBH4) solution. Gold nanoparticles (AuNP) coated with conjugated gum Arabic (GA) and vincristine (VCR) were successfully synthesized and characterized. The conjugation of GA-VCR and AuNP displayed a narrow hydrodynamic particle size distribution with average size < 100 nm by TEM and PSA (particle size analyzer). We investigated the cytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle by tetrazolium salt assay (MTT) using cancer cell line CCR-CEM. Cytotoxic activity of conjugated VCR-GA-AuNP before and after purification by Size Exclusion Chromatography (SEC), against leukemia cell line CCRF-CEM, was described by IC50 value. All formulation had a cytotoxic of activity with IC50 <20 μg/ml. The IC50 of samples against CCRF cell line were 1,026 μg/mL and 2,607 ug/mL, respectively.

Formulation and evaluation of the nanosilver gel using bioreductor ethanol extract of katuk leaf (sauropus androgynus (l) merr) as antibacterial

“…It was shown that nanoparticles with a potential above (+/-) 30 mV are stable in suspension because the surface charge prevents the aggregation of particles (Pertiwi et al, 2018). The zeta potential analysis shows relevant results and confirms the distribution pattern data and the polydispersity index.…”

Review: Produksi, Karakterisasi Dan Aplikasi Selulosa Bakteri Di Bidang Farmasi

“…Tingginya zeta potensial disebabkan oleh adanya gugus karboksil yang diperoleh dari proses oksidasi cincin piranosa (Yan et al, 2017). Zeta potensial merupakan suatu parameter yang menunjukkan adanya gaya tolak antar partikel, umumnya digunakan untuk mengetahui sifat muatan permukaan pertikel (Pertiwi et al, 2018).Semakin tinggi zeta potensial, maka emulsi semakin stabil karena dapat mencegah terjadinya flokulasi. Semakin tinggi konsentrasi selulosa bakteri yang ditambahkan, maka emulsi yang distabilkan dengan ukuran kecil semakin homogen.…”

Utilization of tekelan leaf extract (Chromolaena odorata L.) based on nanoliposome technology as treatment of antihyperglycemia

“…Abinav et al [10] stated that the size of the vesicle diameter is influenced by the amount of cholesterol, the solvent, and the surfactants that form it. [11] stated that the particle size after purification showed smaller results compared to that before purification. This indicates that purification has resulted in pure nanoparticles.…”