PEMBUATAN KULTUR SEL PRIMER DARI SIRIP EKOR IKAN MAS (Cyprinus carpio)

Sumiati, Tuti; Gardenia, Lila; Sunarto, Agus

doi:10.15578/jra.4.1.2009.107-116

Cited by 3 publications

(6 citation statements)

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“…Although cell initiation has taken place and cells began to spread on the surface of the flask, its development was not perfect. According to Sumiati et al, (2009), medium replacement which was too soon in which the cells had not completely attached to the flask, was thought to be the cause of the cells not growing properly and eventually dying.…”

Section: Resultsmentioning

confidence: 99%

“…The confluence of gill primary cell culture and caudal fin primary cell culture can reach more than 50% at the 17 th passage so that it can be done passage. According to Sumiati et al (2009) passage was only done after reaching a growth rate (confluence) of more than 50%.…”

Section: Culture Of Primary Kidney Cellsmentioning

confidence: 99%

“…The media used in the primary culture as quite similar to media for maintenance, in which only the serum level used was reduced to 10%. According to Sumiati et al, (2009), growth media for producing primary cell culture should contain 20% serum levels to stimulate the growth of cells until it developed into a monolayer. Moreover, for cell maintenance, the serum can be as much as 10%.…”

Section: Table 2 Development Of Primary Cells In Culture Flasksmentioning

confidence: 99%

“…According to Sumiati et al (2009), primary cell lines of carp's caudal fin has successfully been produced. However, the cell culture cannot be maintained or preserved; thus, many of them finally died.…”

Section: Introductionmentioning

confidence: 99%

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Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)

Insariani

Trisniaty

Riatmono

et al. 2020

JIPK

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HighlihgtDevelop primary cultures derived From tissue tails fins, gills, kidney and spleen from local Indonesian carp (Cyprinus carpio).Primary culture cell with L15 Mediacell cultures consist of two type Fibroblast-like and epithelial –like cell AbstractThe fish cell lines technology have been developed for the interests of the fisheries world. This study aimed at developing a primary cell line from gill, kidney, spleen, and caudal fin of a common carp (Cyprinus carpio). A healthy common carp weighing 20 g (~1 month) was collected from the Cijeruk Fish Seed Center, Bogor. The development of primary cell lines from the gill, fin, tail, kidney and spleen tissue was performed in cell culture medium Leibovitz’s L-15 supplemented with 20% serum fetal bovine, 250 IU Penicillin, 250 µg / ml kanamycin sulfate and 2Mm L-Glutamine, and incubated at 28°C. Primary cell lines of caudal fin and gill began to form a monolayer on day 17 after culture. While the development of cell lines from kidney and spleen, although the initiation of cells and cells spread on the surface into a monolayer, was not perfect; therefore, the passage was unable to be done. Microscopic observations and Giemsa staining showed primary cell lines of caudal fin and gill based on cell morphology consisted of two cell types, fibroblast-like cells and epithelial-like cells. The first passage was done on day 17 when the confluence was more than 50%. The next passage was carried out every 3 weeks when confluence reached 70% -80%. The primary cell culture of gill was successfully passaged as much as 72 and the caudal fin was successfully passed as much as 89 times over 7 years. These new cell lines can be further used to propagate fish viruses and other biotechnology assays.

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Section: Resultsmentioning

confidence: 99%

Section: Culture Of Primary Kidney Cellsmentioning

confidence: 99%

Section: Table 2 Development Of Primary Cells In Culture Flasksmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)

Insariani

Trisniaty

Riatmono

et al. 2020

JIPK

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“…In the in vitro culture process, cells originating from tissues or organs that have been mechanically or enzymatically broken down become cell suspensions which are cultured and develop into a single layer of tissue (monolayer) on a hard surface such as a flask and can be renewed through subculture. (passages) in order to obtain sustainable cells (cell line) (Sumiati et al, 2009). The cell culture passage process is important for the cell culture laboratory.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Goat Ovarium Granulosa Cells With Extract Leaves ‎‎(Schleichera oleosa) at Different Passage Levels in Vitro

Hanifah

Mutiah

2020

El–Hayah

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S. oleosa belongs to the Sapindaceae family and has phytochemicals phenolic acid so that it has enormous benefits in the process antioxidant. The presence of antioxidant activity in cells can affect the defense of the cell protein membrane, so that the ability of cells to perform division is optimal. In vitro this is mostly done by adding hormones to cell culture media or natural compounds to increase cell proliferation. Every population of cell that has undergone division during the proliferation process to confluent is an indication that the cell is aging. This study was an experimental study was designed using a completely randomized design (CRD) with 5 treatments and 5 replications as follows: K0: granulose cells and culture media without treatment (0 µl), P1: granulose cells and culture media with 1% kesambi leaf extract. (30 µl), P2: granulose cells and culture media given kesambi leaf extract 1.5% (45 µl), P3: granulose cells and culture media given kesambi leaf extract 2% (60 µl) and P4: granulosa cells and The culture media was given 2.5% (75 µl) to the leaves extract., the characteristics of goat ovarian granulosa cells by giving kesambi (Scheichera oleosa) leaf extract at different passages were at the level of confluence showing the highest value in passage I, namely treatment P1. Meanwhile, the level of viability shows the highest value in section I, namely the P4 treatment

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Efektivitas Daya Simpan dan Uji Organoleptik Nugget Ikan Gabus

Rumaseb,

Tampubolon,

Suprayitno

2023

jharma

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The problem of malnutrition in Papua is caused by economic factors and parents' limited knowledge about nutrition. The impact of malnutrition on children under five causes growth and development to be hampered and can interfere with productivity. Providing food with high protein is an alternative to improve the nutritional status of children under five with infectious disease problems. One of the foods that contains high protein and albumin which is a local food and is easy to obtain in the Papua region is snakehead fish. Objective: To determine the effectiveness of storage and organoleptic tests on snakehead fish nuggets. Methods The research design used in this study used the factorial analysis method. The research design used in this study used the factorial analysis method by comparing the four treatments with three repetitions for each treatment. The four treatments are looking for processing results without using preservatives stored in open spaces and in refrigerators and processing results using preservatives stored in open spaces and in refrigerators. In each treatment three repetitions were carried out to obtain valid conclusions, namely the 1st test (first day), 2nd test (third day) and 3rd test (fifth day). The data analysis used was the mean comparison between treatments without preservatives in the open space and in the refrigerator and using preservatives in the open space and in the refrigerator. The significance test used the Kruskall Wallis test followed by the Friedman test. Results: The difference test for snakehead fish nuggets without preservatives showed a significant difference compared to fish nuggets without preservatives with a p value <0.05. Conclusion: The most optimal storage time for cork fish nuggets without preservatives or with preservatives in an open space or normal temperature can last up to the 3rd day.

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PEMBUATAN KULTUR SEL PRIMER DARI SIRIP EKOR IKAN MAS (Cyprinus carpio)

Cited by 3 publications

References 4 publications

Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)

Development of Primary Cell Lines from Gill, Kidney, Spleen and Caudal Fin of Common Carp (Cyprinus carpio)

Characteristics of Goat Ovarium Granulosa Cells With Extract Leaves ‎‎(Schleichera oleosa) at Different Passage Levels in Vitro

Efektivitas Daya Simpan dan Uji Organoleptik Nugget Ikan Gabus

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