Penaeicola halotolerans gen. nov., sp. nov., a novel bacterium of the family Cyclobacteriaceae isolated from a shrimp pond

Abstract

A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium (designated as LMIT005T) was isolated from shrimp ponds in Shantou, China. The new isolate was characterized taxonomically using a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain LMIT005T was found to be affiliated with the family Cyclobacteriaceae of the order Cytophagales while appearing as a distinct lineage. The 16S rRNA gene sequence similarity between strain LMIT005T and Algoriphagus yeomjeoni KCTC 12309T, the closest type strain in the family, was 91.3 %. Strain LMIT005T grew optimally at 25 °C, pH 7 and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content (data from genome sequence) was 40.5 mol%. Compared with reference strain A. yeomjeoni KCTC 12309T, the average nucleotide identity (ANI) of LMIT005T was 70 %. The sole respiratory quinone of LMIT005T was menaquinone (MK-7), and the major fatty acids were summed feature 3 (C16 : 1 ω6c / C16 : 1 ω7c). The polar lipids of strain LMIT005T were mainly composed of phosphatidylethanolamine, phosphatidylcholine, two unidentified amino lipids, two unidentified lipids, one unidentified glycolipid and one unidentified phospholipid. The draft genome of strain LMIT005T comprised 3 089 781 bp (3.09 Mb) nucleotides and 2773 genes. Antimicrobial resistant-related genes (blal, mexA, and mexb) were annotated in the genome of strain LMIT005T, which indicated that it might be able to resist β-lactam antibiotics. This was further verified by antimicrobial resistant test. Given its distinct genomic, morphological, and physiological differences from previously described type strains, strain LMIT005T is proposed as a representative of a novel genus of the family Cyclobacteriaceae, with the name Penaeicola halotolerans gen. nov., sp. nov. The type strain is LMIT005T (=KCTC 82616T=CICC 25047T).

Bacterial community structure and bacterial isolates having antimicrobial potential in shrimp pond aquaculture

“…Halobacterium salinarum JCM 8978 (AB663362) was used as an outgroup. Bootstrap support is shown for cases where the value was greater than 50% based on 1000 replications (Ali et al 2021). The scale bar indicates 0.01 substitution per nucleotide position.…”

Identification of antibacterial metabolites produced by a marine bacterium Halobacillus marinus HMALI004

“…After being 10-fold serially diluted, dilutions (10 −3 , 10 −4 , and 10 −5 ) were spread on marine agar 2216E (Difco, Becton, Dickinson and Company) for bacterial isolation. The HMALI004 strain was selected out of 20 bacterial strains after 3 days of incubation at 25°C under aerobic conditions based on distinct morphological features and purified after three successive transfers to a fresh medium (Ali et al, 2021). The pathogenic bacterial strains V. parahaemolyticus and V. cholera were kindly provided by the Guangdong Provincial Key Laboratory of Marine Biotechnology, China, and stored in our laboratory at −80°C.…”

Shiella aurantiaca gen. nov., sp. nov., a novel bacterium isolated from activated sludge, and proposal of Shiellaceae fam. nov.

“…General characteristics of the genus Shiella and the closely related genera (families in parentheses) of the order Cytophagales Taxa: 1, Shiella (Shiellaceae) [this study]; 2, Penaeicola (Cyclobacteriaceae)[54]; 3, Algoriphagus (Cyclobacteriaceae)[12,44,46]; 4, Sporocytophaga (Cytophagaceae)[17]; 5, Roseivirga (Roseivirgaceae)[7,55,56]; 6, Marinoscillum (Reichenbachiellaceae)[8,47]; 7, Fulvivirga (Fulvivirgaceae)[49][50][51]; 8, Nafulsella (Cesiribacteraceae)[48]; 9, Adhaeribacter (Hymenobacteraceae)[52,53]. +, Positive; −, negative; nd, not determined or data not available.…”