Penaeus vannamei protease stabilizing process of ZnS nanoparticles

Razzaghi, Mozhgan; Homaei, Ahmad; Mosaddegh, Elaheh

doi:10.1016/j.ijbiomac.2018.01.173

Cited by 29 publications

(14 citation statements)

References 49 publications

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“…Numerous non-conventional techniques, such as ultrasound-assisted extraction (UAE) [ 9 , 10 ], pressurized liquid extraction (PLE) [ 11 ], supercritical fluid extraction (SFE) [ 12 ], and microwave-assisted extraction (MAE) [ 13 ], fulfill these requirements. In comparison with other techniques, MAE has its advantages, such as low cost and lower extraction time; thus, it can be categorized as “green technology” according to environmental policies [ 14 , 15 ]. The extraction time, temperature, technique, solid-to-liquid ratio, and solvent concentration are significant variables that, individually or in combination, alter the extraction efficiency.…”

Section: Introductionmentioning

confidence: 99%

Metabolite Profiling of Microwave-Assisted Sargassum fusiforme Extracts with Improved Antioxidant Activity Using Hybrid Response Surface Methodology and Artificial Neural Networking-Genetic Algorithm

et al. 2022

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Sargassum fusiforme (SF) is a popular edible brown macroalga found in Korea, Japan, and China and is known for its health-promoting properties. In this study, we used two sophisticated models to obtain optimized conditions for high antioxidant activity and metabolite profiling using high-resolution mass spectrometry. A four-factor central composite design was used to optimize the microwave-assisted extraction and achieve the maximum antioxidant activities of DPPH (Y1: 28.01 % inhibition), ABTS (Y2: 36.07 % inhibition), TPC (Y3: 43.65 mg GAE/g), and TFC (Y4: 17.67 mg CAE/g), which were achieved under the optimized extraction conditions of X1: 47.67 %, X2: 2.96 min, X3: 139.54 °C, and X4: 600.00 W. Moreover, over 79 secondary metabolites were tentatively identified, of which 12 compounds were reported for the first time in SF, including five phenolic (isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate, 3,4-dihydroxyphenylglycol, scopoletin, caffeic acid 4-sulfate, and cinnamoyl glucose), two flavonoids (4’,7-dihydroxyisoflavone and naringenin), three phlorotannins (diphlorethohydroxycarmalol, dibenzodioxin-1,3,6,8-tetraol, and fucophlorethol), and two other compounds (dihydroxyphenylalanine and 5-hydroxybenzofuran-2(3H)-one) being identified for the first time in optimized SF extract. These compounds may also be involved in improving the antioxidant potential of the extract. Therefore, optimized models can provide better estimates and predictive capabilities that would assist in finding new bioactive compounds with improved biological activities that can be further applied at a commercial level.

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Section: Introductionmentioning

confidence: 99%

Metabolite Profiling of Microwave-Assisted Sargassum fusiforme Extracts with Improved Antioxidant Activity Using Hybrid Response Surface Methodology and Artificial Neural Networking-Genetic Algorithm

et al. 2022

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“…The caseinolytic activity was measured according to previous studies [4,27,28], with some modifications. Briefly, in 2 ml microtubes (n = 4), 0.5 ml of papain solution (prepared in 100 mM sodium phosphate buffer, pH 7.5) was mixed with 0.5 ml of 0.65% (w v −1 , pH adjusted to 7.5) casein solution and incubated for 5 min at 37°C.…”

Section: Caseinolytic Activity Assaymentioning

confidence: 99%

In vitro effect on the proteolytic activity of papain with proteins of the skin as substrate

Trevisol

Henriques

Cesca

et al. 2022

Intern J of Cosmetic Sci

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Objective This work aims to evaluate the effects of enzyme concentration, pH, temperature and time course degree of hydrolysis (DH) of papain regarding further development of pharmaceutical and cosmetic formulations. Methods The hydrolysis of casein, collagen, keratin and porcine skin at pH and temperature ranges of the human skin was evaluated. Also, low contact times of enzyme–substrate were studied. The incorporation of 3mM of cysteine improved the caseinolytic (PU), collagenolytic (CU) and keratinolytic (KU) activities of papain. Results: In general, the increase from 0.1 to 1.0 or 2.0 mgmL−1 of papain improved PU, CU and KU. When 2.0 mgmL−1 of papain was used, the highest DH of casein, collagen and keratin was obtained at 240min (14%, 35% and 6% respectively). The decrease in pH and temperature reduced all proteolytic activities, but papain maintained at least 50% and 40% of its activity at 26°C and pH 4.5 respectively. Scanning electron micrographs of the surface of the skin showed that papain application had exfoliating activity. Conclusion This preformulation study demonstrated that papain concentration, time of application and pH of the product should be evaluated when developing a product to promote the hydrolysis of the proteins of the skin.

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“…The percentage residual activity of this bromelain was determined under standard assay conditions. The result described above is particularly important as the loss of enzyme activity and catalytic efficiency over time is a major issue encountered in the field of biotechnology [61].…”

Section: Biochemical and Thermodynamic Properties Of The Partially Pu...mentioning

confidence: 99%

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Silva¹,

Nerys²,

Silva³

et al. 2022

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Macambira plant (Bromelia laciniosa Mart. Ex Shult. & Schult. f.) is a species belonging to the family Bromeliaceae, which is distributed widely in the hinterlands of northeastern Brazil, from Bahia to Piauí states. The plants of this species contain an enzyme complex named bromelain, which has great potential for therapeutic applications. In the present study, bromelain derived from Bromelia laciniosa leaves was assessed for its thermodynamic properties and in vitro cytotoxic and healing activities. Bromelain was extracted from the leaves and partitioned using ethanol. The partially purified bromelain was then characterized to determine the optimum pH and temperature and the kinetic and thermodynamic parameters. The partially purified bromelain derived from macambira leaves exhibited its highest activity at 47°C and pH 7, when the Km and Vmax values of 2.26 µM and 2.083 U/mL, respectively. The half-life of this bromelain at 47°C was 5.78 h. At 47°C, the Gibbs's free energy, enthalpy, and entropy values reached 89, 53.54, and –0.11 kJ/mol K, respectively. In the SDS-PAGE analysis, bromelain appeared as the main protein band, with an estimated molecular mass of 33 kDa. The in vitro cytotoxic activity and wound healing effects were determined using the MTT assay and Scratch assay, respectively. These assays were performed on the non-neoplastic cell lines L-929 and PBMC and the human tumor cell lines MCF-7, HCT-116, and NCI-H292. Bromelain exhibited a positive healing activity with a change rate similar to that observed for the control. In addition, bromelain exhibited cytotoxic activity against NCI-H292 tumor cells and chemopreventive potential. These results indicate that bromelain derived from macambira leaves may be utilized as a potential ingredient in pharmaceutical formulations, for various healing and chemopreventive purposes and as an alternative to the bromelain extracted from pineapple. In addition, the biochemical characteristics of this bromelain could find application in the food industry. Future studies should, therefore, focus on deciphering the action mechanisms underlying the activities of bromelain observed in the present study to further confirm and enhance its application potential for therapeutic purposes.

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Penaeus vannamei protease stabilizing process of ZnS nanoparticles

Cited by 29 publications

References 49 publications

Metabolite Profiling of Microwave-Assisted Sargassum fusiforme Extracts with Improved Antioxidant Activity Using Hybrid Response Surface Methodology and Artificial Neural Networking-Genetic Algorithm

Metabolite Profiling of Microwave-Assisted Sargassum fusiforme Extracts with Improved Antioxidant Activity Using Hybrid Response Surface Methodology and Artificial Neural Networking-Genetic Algorithm

In vitro effect on the proteolytic activity of papain with proteins of the skin as substrate

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

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