Penambahan PGF2α dalam pengencer semen dapat meningkatkan motilitas pasca thawing motility spermatozoa domba Waringin

Abstract

<p class="MDPI17abstract"><strong>Objective: </strong>The purpose of this research was to find out the rise of spermatozoa motility of Waringin sheep which was given additional amount of PGF2α in commercial semen diluents (Andromed).</p><p class="MDPI17abstract"><strong>Methods: </strong>A total of 3 Waringin sheep aged 2-3 years were used in this study. Semen samples were collected using an artificial vagina and evaluation of the quality of the spermatozoa was carried out macroscopically and microscopically. This study used a unidirectional completely randomized design. After evaluated, semen samples were added with Andromed diluents afterwards divided into three groups treatment, K1; K2; and K3 where each group was then added with physiologic NaCl, 37,5 µg PGF2α, and 75 µg PGF2α. All of the samples were equilibrated in refrigerator for 4 hours and spermatozoa motility was examined, then after the freezing process was carried out and stored for 24 hours, the post thawing spermatozoa motility was examined. The data obtained was analyzed using one-way analysis of variance (ANOVA) and followed by Duncan test.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result of the research showed that motility of post equilibration spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 43,67±1,20; 75,00±4,00; and 82,33 ±3,18 (P<0,05); and motility of post thawing spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 25,33±1,85; 49,33±3,17; dan 54,00 ±5,50 (P<0,05).<strong></strong></p><strong>Conclusions: </strong>In conclusion that was the rise in motility of prefreezing spermatozoa and motility of post thawing spermatozoa of Waringin sheep which given additional amount of PGF2α on Andromed diluents.

Comparison of Invivo and Invitro PGF2α Administrations on The Sperm Quality of Gembrong Goats

“…In vivo administration of PGF2α can increase the motility of frozen spermatozoa of Nubian goats after thawing [12]. In addition, an increase in the quality of spermatozoa due to the addition of PGF2α in vitro to the diluent medium has been reported in Nubian goat [15] and in Waringin sheep [13]. Therefore, it is necessary to conduct a study that compares the effectiveness of two administration methods on the same breed of goats to obtain the most effective method to improve the semen quality of Gembrong goats.…”

“…Spermatozoa quality can be improved in various ways. Recent studies revealed that the addition of prostaglandin F 2 D (PGF 2 D) can enhance the quality of spermatozoa through in vivo [12][13][14] and in vitro [15] administrations because PGF 2 D can work directly or indirectly.…”