Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro

Wibowo, Risky Hadi; Mubarik, Nisa Rachmania; Rusmana, Iman; Thenawidjaya, Maggy

doi:10.14692/jfi.13.3.105

Cited by 12 publications

(15 citation statements)

References 10 publications

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“…The differences in the chitinolytic index of the isolates were due to differences in the activity of the chitinase enzyme from each of these isolates. The clear zone began to appear on the first day, and the zone size increased until the fifth day of observation (Wibowo, Mubarik, Rusmana, Thenawidjaya, 2017).…”

Section: Chitinase Activity Assay In the Chitin Agarmentioning

confidence: 99%

“…Microorganisms with chitinolytic abilities are believed to be able to play a role in controlling the attack of pathogenic fungi by making chitin a source of carbon and nitrogen (Jholapara, Mehta, Bhagwat, and Sawant, 2013). Several groups of bacteria and fungi with chitinolytic abilities are used in controlling plant pathogens such as Bacillus (Wibowo, Mubarik, Rusmana, Thenawidjaya, 2017, Pseudomonas (Saranya et al, 2013), Trichoderma (Agrawal et al, 2012, Toshy et al, 2012 and Streptomyces (Arias et al, 2016). Pattanapipitpaisal & Songklanakarin (2012) stated that S. hygroscopicus in vitro was antagonistic to Colletotrichum gloeosporioides and Sclerotium rolfsii and inhibited the growth of pathogenic fungi with the activity of hydrolytic enzymes such as chitinase and glucanase.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens

Wibowo

Sipriyadi

Mubarik

et al. 2020

EKW

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Chitinolytic actinobacteria are currently more widely used because of their ability as the biological control agents to the pathogenic fungi, especially in horticultural and plantation crops. This research was conducted to obtain isolates of chitinolytic soil actinobacteria from the rhizosphere of the rubber plant (Hevea brasiliensis) area in IPB University. Antifungal activities from these actinobacteria hopely able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum and Sclerotium rolfsii are used in the inhibition test of chitinolytic actinobacteria. The results successfully obtained 16 isolates of actinobacteria were grew on Humic Acid Vitamin (HV) agar. It showed that six of 16 actinobacteria isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA). KK-15 and KK-07 isolates were able to produce the largest inhibition percentages in F. oxysporum and S. rolfsii. Based on the chitinolytic index (CI) values, KK-15 and KK-07 isolates produced CI values of 1.25 and 1.5, respectively. The morphological characteristics and Gram staining of both KK-15 and KK-07 isolates are closely relative with Streptomyces sp. Abstrak: Bakteri kitinolitik saat ini banyak diteliti karena kemampuannya sebagai agens pengendali hayati jamur patogen khususnya pada tanaman hortikultura dan perkebunan. Penelitian ini dilakukan untuk mendapatkan isolat-isolat bakteri kitinolitik asal tanah dari bagian perakaran tanaman karet (Hevea brasiliensis) di daerah perkebunan karet Institut Pertanian Bogor (IPB). Aktivitas antifungi dari bakteri ini diharapkan mampu menghambat pertumbuhan jamur patogen tanaman secara in Vitro pada media agar-agar kitin. Fusarium oxysporum dan Sclerotium rolfsii digunakan dalam uji hambat aktinobakteri kitinolitik. Hasil penelitian memperoleh 16 isolat aktinobakteri berhasil diisolasi dengan menggunakan media agar-agar Humic Acid Vitamin (HV), dan dari 16 isolat tersebut, enam isolat mampu menghasilkan zona hambat terhadap pertumbuhan hifa dari kedua jamur patogen pada media Potato Dextrose Agar (PDA) secara in Vitro. Isolat KK-15 dan KK-07 mampu menghasilkan diameter hambatan terbesar pada jamur F. oxysporum dan S. rolfsii. Berdasarkan nilai index kitinolitik (IK), isolat KK-15 dan KK-07 menghasilkan nilai IK sebesar 1,25 dan 1,5 secara berurutan. Karakteristik morfologi dan pewarnaan Gram dari kedua isolat yaitu KK-15 dan KK-07 memiliki kedekatan dengan kelompok Streptomyces spp.

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Section: Chitinase Activity Assay In the Chitin Agarmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens

Wibowo

Sipriyadi

Mubarik

et al. 2020

EKW

Self Cite

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“…Testing of chitinase crude extract was carried out using agar well diffusion method (Wibowo et al, 2017). Chitinolytic bacteria were grown on 150 mL NB medium and incubated in a room temperature orbital shaker at a speed of 120 rpm for 72 hours, then 10 mL of culture were centrifuged at 4000 rpm for 20 minutes.…”

Section: Synergy Test Of Ub Forest Chitinolytic Bacteriamentioning

confidence: 99%

The Potency of UB Forest Chitinolytic Bacteria to Promote Plant Growth and Inhibit Damping off Disease on Soybean

Roekhan

Dayanti

Oktaviani

et al. 2021

RJLS

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Damping off disease in soybean plants is caused by the fungal Rhizoctonia solani. The damping off disease causes a yield loss of up to 85-100%. The purpose of this study was determining the ability chitinolytic bacteria consortium of UB Forest's in suppressing damping off disease in vitro and in vivo as well as its potential to stimulate the growth of soybean plants. The research stages included isolation of the pathogenic fungus R. solani and the pathogenicity test. Rejuvenation chitinolytic bacterial isolates of UB Forest, test chitinolytic bacteria antagonist of UB Forest against R. solani fungus, test of Plant Growth Promoting (PGP) activities, synergy test of selected chitinolytic bacterial isolates, in vitro test for the inhibition of chitinase crude extract against R. solani, and inhibition test of chitinolytic bacteria consortium against damping off disease. The selected chitinolytic bacteria were code bacteria UB12, UB19, and UB52 with plant growth promoting activities with inhibition percentage of the pathogen R. solani of 73.9%, 67.4%, and 71.7%. The best chitinolytic bacterial isolates were the genus Bacillus sp. and Pseudomonas sp. The inhibition test of chitinase crude extract showed an inhibition percentage of 25-55%.

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“…Bakteri tanah kitinolitik saat ini banyak diteliti karena kemampuannya sebagai agens pengendali hayati jamur patogen khususnya pada tanaman. Penelitian Wibowo et al, (2017) bakteri kitinolitik yang dilakukan di Taman Nasional Bukit Dua Belas (TNBD) dan perkebunan sawit di Jambi telah mendapatkan isolat potensial penghasil senyawa antijamur terhadap Ganoderma boninense secara in Vitro, namun laporan terkait potensi bakteri kitinolitik yang mampu menghambat pertumbuhan jamur Fusarium dan Sclerotium belum ada dilaporkan sampai saat ini, hal ini menjadi satu alasan kuat penelitian ini perlu dilakukan.…”

Section: Hambat Bakteri Kitinolitik Bakteri Diisolasi Dan Ditapis Daunclassified

Soil Chitinolytic Bacteria from Jambi Province to Produce Antifungal of Plant Pathogens

Wibowo

Sipriyadi²,

Mubarik³

et al. 2020

mangiferaedu

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Chitinolytic bacteria are bacteria that have chitinolytic activity, which is able to hydrolysis the composition of chitin which composes many fungal cell walls. Chitinolytic bacteria are currently more widely used because of their ability as a biological control agent to the pathogenic fungi especially in horticultural and plantation crops. This research was conducted with the aim of obtaining isolates of chitinolytic bacteria that were able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum, Sclerotium rolfsii, and Rhizoctonia solanii are used in the inhibition test of chitinolytic bacteria. Bacteria were isolated and screened from the soil of Bukit Dua Belas National Park and Oil Palm Plantations in Jambi using 0.3% chitin agar media. The results showed that two of 10 bacterial isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA) media. TB04-13 isolate was able to produce the largest inhibition in F. oxysporum and R. solanii about 42% and 42.05% respectively, while TB04-15 isolate produced the biggest inhibition in S. Rolfsii ranged to 25.50%. Based on the chitinolytic index (CI) values, isolates TB04-13 and TB04-15 produced CI values of 1.60 and 0.63, respectively. The morphological characteristics and Gram staining of both TB04-13 and TB04-15 chitinolytic isolates are included in rod-shaped and Gram-positive bacteria. Both of these isolates can be used as antifungal-producing candidates for plant pathogenic fungi in Indonesia.

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Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro

Cited by 12 publications

References 10 publications

Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens

Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens

The Potency of UB Forest Chitinolytic Bacteria to Promote Plant Growth and Inhibit Damping off Disease on Soybean

Soil Chitinolytic Bacteria from Jambi Province to Produce Antifungal of Plant Pathogens

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