Pencegahan Lesi Aterosklerosis Oleh Asam Alfa Lipoat Pada Aorta Mencit Jantan (Mus Musculus) Yang Diberi Diet Tinggi Kolesterol

Abstract

Atherosclerosis can happen easily on animal that are given high cholesterol diet. Alpha lipoic acid is universal antioxidant that very effective to reduce free radicals, include lipid peroxide. The goal of this research was to find out the prohibition of atherosclerosis lesion development on male mice aorta that was given high cholesterol diet by alpha lipoic acid. This was an experimental laboratory research with post test only design. Eighteen adult male mice were segregated into three groups labelled as group NaCl 0,9%, yolks and ALA.. The experiment was design for 6 weeks. The measured parameter was atherosclerosis lesion score of groups labelled. The statistical test result showed the significant difference of some tested groups. There was a statistically significant difference between alpha lipoic acid and yolks group (p=0,008), but there is no significant difference was found between ALA and NaCl 0,9% group (p=0,126). As the conclusion, this study proves that alpha lipoic acid has the effects to prohibition of atherosclerosis lesion development on male mice aorta that are given high cholesterol diet.

The effect of alpha-lipoic acid on expression of VCAM-1 in type 2 diabetic rat

“…In this research, ALA 60 mg/kg dosage from the ALA dose conversion of previous research was utilized [19]. The research result designates that ALA administration for 60 mg/kg/day for 3 weeks is effective to decrease the oxLDL concentration for diabetic rats.…”

“…The results of this present study are also consistent with the earlier study by Heinisch et al (2010) [24], in which ALA treatment (21 days of intravenous treatment with 600 mg ALA) improves endothelium-dependent vasodilatation in patients with type 2 diabetes. Furthermore, in high-fat-fed mice, lipoic acid (60 mg/kg body weight for 2 weeks) has been demonstrated to have protective effects on atherosclerosis [19].…”

“…In the present investigation, ALA was administered 60 mg/kg body weight every day for 3 weeks. Oral treatments were performed using a sonde [19].…”

Pengaruh Pemberian Asam Alfa Lipoat terhadap Kadar Kolesterol Total pada Tikus Diabetes Melitus Tipe 2

“…Kedua, ALA dapat meningkatkan reseptor LDL dihati sehingga meningkatkan pengambilan kolesterol LDL oleh hati. 21 Ketiga, ALA dapat meningkatkan aktivitas LPL sehingga menurunkan produksi kolesterol VLDL.Keempat, ALA dapat menurunkan lipogenesis di hati dengan menurunkan ekspersi gen lipogenik seperti ChREBP dan SREBP-1C. Kelima, ALA dapat meningkatkan aktivitas LCAT yang berperan dalam esterifikasi kolesterol dari jaringan yang akan diangkut oleh HDL sehingga meningkatkan pengambilan kolesterol kehati melalui pertukaran HDL dan VLDL.…”

“…Pada penelitian sebelumnya, Ismawati dkk tahun 2011 membuktikan bahwa ALA mampu mencegah pembentukan lesi aterosklerosis pada aorta mencit diabetes yang diberi diet tinggi kolesterol. 21 Penelitian Ismawati dkk tahun 2017 juga membuktikan ALA mampu mencegah pembentukan lesi aterosklerosis pada aorta abdominalis tikus yang sama pada penelitian ini. 20 Hal ini membuktikan bahwa ALA berpotensi untuk mencegah komplikasi dari diabetes melitus terutama PJK dari berbagai jalur metabolisme sehingga memungkinkan untuk dapat menurunkan angka kematian pasien DM akibat PJK.…”