PENELUSURAN FUNGI ENDOFIT PADA DAUN KOPASANDA (Chromolaena odorata L.) YANG BERPOTENSI SEBAGAI  PENGHASIL ANTIBAKTERI TERHADAP BAKTERI   PENYEBAB  INFEKSI KULIT

Rusli, Rusli; Kosman, Rachmat; Melinda, Pina

doi:10.33096/jifa.v12i1.622

Cited by 1 publication

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“…Bakteri hasil peremajaan disuspensikan dengan larutan NaCl fisiologis 0,9% hingga diperoleh transmitan 25% menggunakan spektrofotometer dengan panjang gelombang 580 nm dan digunakan larutan NaCl fisiologi 0,9% sebagai blanko 12 .…”

Section: Penyiapan Bakteri Ujiunclassified

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Isolation and Identification Endophyte Fungi from Daun Nona Makan Sirih (Clerodendrum thomsoniae) as Antibacteril Against Bacteria Causing Skin Infection Using TLC-Bioautography

Hadjar,

Fitriana,

Asmaliani

2024

j. microbiol. sci

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Nona makan sirih (Clerodendrum thomsoniae) contains secondary metabolites that have the potential as antibacterials. The TLC-Bioautography method will determine the antibacterial activity and chemical components of endophytic fungal isolates of nona makan sirih. This research involved the isolation of endophytic fungi from leaves of nona makan sirih using PDAC media, followed by purification and macroscopic and microscopic tests. In addition, a screening test its effect againts pathogenic bacteria. The chosen isolates were fermented for 21 days. Then supernatant and mycelia were seperated. The supernatant was extracted by dissolving ethyl acetate using the liquid-liquid ectraction method. Then extract obtained was processed by TLC with the mobile phase of chloroform:methanol (8:1) and TLC-Bioautography was carried out on bacterial tests and chemical components. The results of isolation and purification obtained as many as 13 isolates. Screening test results with IFDNMS codes 6, 8 and 11 showed activity against the test bacteria. TLC-Bioautography result isolate 6 obtained 5 spots with an Rf value of 0.89; 0.65; 0.45; 0.23; 0.05 was active against all test bacteria. Isolate 8 obtained 5 spots with an Rf value of 0.89; 0.65; 0.05 was active against all tested bacteria, while 0.45 was active against P.acnes, S.aureus, and S.epidermidis bacteria and 0.23 against S.aureus bacteria. Isolate 11 obtained 5 spots with an Rf value of 0.89; 0.61; 0.45; 0.23; 0.05 against all test bacteria. The findings indicate that flavonoids and phenolics are chemical components with antibacterial activity.

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Section: Penyiapan Bakteri Ujiunclassified

“…Isolat fungi endofit diinokulasi kedalam medium NA yang berisi bakteri uji, dimana isolat tersebut ditempelkan diatas permukaan media. Kemudian diinkubasi pada suhu 37 o C selama 1x24 jam lalu diamati zona hambat yang terbentuk yang memiliki potensi sebagai antibakteri 12 .…”

Section: Uji Skrining Aktivitas Antibakteri Fungi Endofitunclassified

Isolation and Identification Endophyte Fungi from Daun Nona Makan Sirih (Clerodendrum thomsoniae) as Antibacteril Against Bacteria Causing Skin Infection Using TLC-Bioautography

Hadjar,

Fitriana,

Asmaliani

2024

j. microbiol. sci

View full text Add to dashboard Cite

show abstract

PENELUSURAN FUNGI ENDOFIT PADA DAUN KOPASANDA (Chromolaena odorata L.) YANG BERPOTENSI SEBAGAI PENGHASIL ANTIBAKTERI TERHADAP BAKTERI PENYEBAB INFEKSI KULIT

Cited by 1 publication

References 0 publications

Isolation and Identification Endophyte Fungi from Daun Nona Makan Sirih (Clerodendrum thomsoniae) as Antibacteril Against Bacteria Causing Skin Infection Using TLC-Bioautography

Isolation and Identification Endophyte Fungi from Daun Nona Makan Sirih (Clerodendrum thomsoniae) as Antibacteril Against Bacteria Causing Skin Infection Using TLC-Bioautography

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