Penentuan Kadar Senyawa Aktif Cyanocobalamine Menggunakan Metode Spektrofotometri UV-VIS

Abstract

Cyanocobalmine is vitamin B12 which is widely used to treat or prevent deficiency (deficiency of vitamins) in various types of preparations. Cyanocobalmine in the 5th edition of Indonesian Pharmacopoeia literature can determine by UV-Vis Spectrophotometric method with water solvent. The purpose of this testing was to determine whether the levels of the active compound Cyanocobalamine from the 3 test parameters. In the description test where the sample tested is the same as the Cyanocobalamine specification, namely, crystal powder is dark red. Hot plate was used to determine drying shrinkage. Shrink drying was 2.25% - 2.75% from the ≤ 12% requirement. The determination of the levels of the active compound of Cyanocobalamine fulfills the requirements, namely 96% - 102.0%. The results of the assay showed that the average level of Cyanocobalamine from 4 samples was 96.02%, which met the standards set by the Pharmacopeia. Indonesia Edition V as a requirement for determining the levels of the active compound Cyanocobalamine.

Analisis Kuantitatif Hidrokuinon pada Krim Pemutih di Kota X Menggunakan Spektrofotometri UV–Vis

“…Hidrokuinon dapat diamati menggunakan spektrofotometri UV-Vis karena mengandung kromofor benzena dalam strukturnya (Fahira et al, 2021). Kromofor adalah molekul atau bagian dari molekul yang menyerap cahaya dengan kuat di daerah sinar UV-Vis (Suhartati, 2017). Kromofor benzena pada hidrokuinon akan menyerap sinar monokromatis yang dilewatkan pada kuvet pada panjang gelombang tertentu dan diteruskan, yang dapat dibaca senyawa serapan oleh detektor (Fahira et al, 2021).…”