Dental and oral care often causes injuries that can trigger an inflammatory reaction associated with feeling of discomfort. Drugs that can be given for the treatment of inflammation, namely corticosteroids of the glucocorticoid group and non-steroidal anti-inflammatories (NSAIDs). However, these drugs have side effects, therefore, alternatives with minimal toxicity that can be found in plants are preferrable. Coconut coir has the potential to be an anti-inflammatory drug because it contains flavonoids, tannins and saponins. This study aimed to determine the anti-inflammatory activity of coconut coir extract at concentrations of 50, 100, 150, 500, and 1000 ppm. This was a pure experimental study with a post-test-only control design using blood of rats weighing above 250 grams taken through the retro-orbital sinus. The samples were divided into seven groups, namely 50, 100, 150, 500, 1000 ppm, positive control, and negative control. The results showed that coconut coir extract contained alkaloids, flavonoids, tannins, saponins, and terpenoids. The percentages of inhibition of hemolysis were obtained at 50, 100, 150, 500, and 1000 ppm, namely 14.73%, 24.60%, 38.86%, 43.11%, and 50.39%. In conclusion, coconut coir extract (Cocos nucifera L.) has anti-inflammatory activity. Extract concentration of 1000 ppm has the highest anti-inflammatory activity.
Keywords: inflammation; coconut coir extract; stabilization of red blood cell membranes
Abstrak: Perawatan gigi dan mulut tidak jarang menimbulkan perlukaan yang dapat memicu reaksi inflamasi disertai rasa tidak nyaman. Golongan obat yang dapat diberikan untuk pengobatan inflamasi, yaitu kortikosteroid golongan glukokortikoid dan anti-inflamasi non-steroid (AINS). Namun, obat-obat tersebut memiliki efek samping sehingga dibutuhkan alternatif dengan toksisitas minimal yang dapat ditemukan pada tanaman. Sabut kelapa berpotensi menjadi obat anti-inflamasi karena mengandung flavonoid, tanin, dan saponin. Penelitian ini bertujuan untuk mengetahui aktivitas anti-inflamasi ekstrak sabut kelapa konsentrasi 50, 100, 150, 500, dan 1000 ppm. Penelitian ini merupakan eksperimental murni dengan post-test only control design menggunakan darah tikus yang diambil melalui sinus retro-orbitalis. Kriteria tikus ialah berat di atas 250 gram, yang dibagi menjadi tujuh kelompok, yaitu 50, 100, 150, 500, 1000 ppm, kontrol positif, dan kontrol negatif. Hasil penelitian mendapatkan bahwa ekstrak sabut kelapa mengandung senyawa alkaloid, flavonoid, tanin, saponin, dan terpenoid. Hasil persentase inhibisi hemolisis didapatkan pada 50, 100, 150, 500, dan 1000 ppm, yaitu 14,73%, 24,60%, 38,86%, 43,11%, 50,39%. Simpulan penelitian ini ialah ekstrak sabut kelapa (Cocos nucifera L.) memiliki aktivitas anti-inflamasi. Konsentrasi ekstrak 1000 ppm memiliki aktivitas anti inflamasi tertinggi.
Kata kunci: inflamasi; ekstrak sabut kelapa; stabilisasi membran sel darah merah