Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Buah Parijoto (Medinilla speciosa Blume) dengan Metode Spektrofotometri UV-Vis

Abstract

Parijoto (Medinilla speciosa) has a wide range of pharmacological activities due to its secondary metabolite content, especially flavonoid, and phenolic compounds. Flavonoid and phenolic compounds have a major role in human health with their activities as antioxidants, anti-inflammatories, antimicrobials, etc. This study aimed to determine the total levels of phenolic and flavonoid compounds from the ethanol extract of Parijoto fruit. Parijoto fruit is obtained from Bandungan, Semarang Regency, Central Java. Extraction was carried out by maceration method with 70% ethanol solvent then concentrated using a rotary evaporator. The total phenolic and flavonoid content amount was determined using the colorimetric method with Folin-ciocalteau complex reagent on phenolic with gallic acid as the reference standard and AlCl3 complex reagent on flavonoids with quercetin as the reference standard. Absorbance was measured using a UV-Vis spectrophotometer. The results showed that the color of the viscous extract of Parijoto fruit was dark brown with a yield of 6.67% w/w. The total phenolic content of the ethanol extract of Parijoto fruit was 21.67 µgGAE/g extract and the total flavonoid content was 9.21 µg QE/g extract.

Phytochemical Screening Analysis and Determination of Total Flavonoids and Total Phenolics Content of Ethanol Extract of Sungkai Leaf (Penorema canescens Jack) from Samarinda City

“…Determining the maximum wavelength helps determine the measurement wavelength of a complex compound between quercetin and AlCl3 to provide optimal absorbance (Damayanti et al, 2023). The maximum wavelength in this study was obtained at 443.0 nm.…”

“…Still, on the contrary, if the measurement is carried out after the operating time, there will be a possibility that the complex compound between AlCl3 has been damaged (Suharyanto & Prima, 2020). This operating time measurement aims to determine the time when the quercetin uptake is stable (Damayanti et al, 2023). And obtained in the operating time measurement received a stable absorbance value at 28-32 minutes.…”