Penetration effect of the kINPen plasma jet investigated with a 3D agar-entrapped bacteria model

Liu, Gengliang; Shi, Fukun; Wang, Qun; Zhang, Zhiqiang; Guo, Jinsong; Zhuang, Jie

doi:10.1016/j.microc.2022.107973

Cited by 6 publications

(6 citation statements)

References 60 publications

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“…The hydrophobicity changes highlighted in the WCA analysis can be attributed to the interaction between the MGS surfaces and the RONS produced by CAP 34 , leading to the formation of polar groups. Conversely, the ATR-FTIR analysis did not detect these plasma-induced polar groups.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Helium Plasma in the Post-Covid Era: A Promising Tool for the Disinfection of Silicone Endotracheal Prostheses

da Silva,

do Nascimento,

Milhan

et al. 2023

Preprint

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The COVID-19 pandemic resulted in a high prevalence of laryngotracheal stenosis. The endoluminal tracheal prostheses used to treat this condition are made of medical-grade silicone (MGS). Despite their excellent properties, the main limitation of these prostheses is the formation of a polymicrobial biofilm on their surfaces that interacts with the underlying mucosa, causing local inflammation and interfering with the local healing process, ultimately leading to further complications in the clinical scenario. Cold atmospheric plasma (CAP) shows antibiofilm properties on several microbial species. The present study evaluated the inhibitory effect of CAP on multispecies biofilms grown on MGS surfaces. In addition to the MGS characterization before and after CAP exposure, the cytotoxicity of CAP on immortalized human bronchial epithelium cell line (BEAS-2B) was evaluated. The aging time test reported that CAP could temporarily change the MGS surface wetting characteristics from hydrophilic (80.5°) to highly hydrophilic (< 5°). ATR-FTIR shows no significant alterations in the surficial chemical composition of MGS before and after CAP exposure for 5 min. A significant log reduction of viable cells in mono-species biofilms (log CFU/mL) ofC. albicans, S. aureus, andP. aeruginosa(0.636, 0.738, and 1.445, respectively) was detected after CAP exposure. Multi-species biofilms exposed to CAP showed significant viability reduction forC. albicansandS. aureus(1.385 and 0.831, respectively). The protocol was not cytotoxic to BEAS-2B. It could be concluded that CAP can be a simple and effective method to delay the multi-species biofilm formation inside the endotracheal prosthesis.

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Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Helium Plasma in the Post-Covid Era: A Promising Tool for the Disinfection of Silicone Endotracheal Prostheses

da Silva,

do Nascimento,

Milhan

et al. 2023

Preprint

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show abstract

“…The hydrophobicity changes highlighted in the WCA analysis can be attributed to the interaction between the MGS surfaces and the RONS produced by CAP [ 35 ], leading to the formation of polar groups. Conversely, the ATR-FTIR analysis did not detect these plasma-induced polar groups.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Helium Plasma in the Post-COVID-19 Era: A Promising Tool for the Disinfection of Silicone Endotracheal Prostheses

Silva,

Do Nascimento,

Milhan

et al. 2024

Microorganisms

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Despite the excellent properties of silicone endotracheal prostheses, their main limitation is the formation of a polymicrobial biofilm on their surfaces. It can cause local inflammation, interfering with the local healing process and leading to further complications in the clinical scenario. The present study evaluated the inhibitory effect of cold atmospheric plasma (CAP) on multispecies biofilms grown on the silicone protheses’ surfaces. In addition to silicone characterization before and after CAP exposure, CAP cytotoxicity on immortalized human bronchial epithelium cell line (BEAS-2B) was evaluated. The aging time test reported that CAP could temporarily change the silicone surface wetting characteristics from hydrophilic (80.5°) to highly hydrophilic (<5°). ATR-FTIR showed no significant alterations in the silicone surficial chemical composition after CAP exposure for 5 min. A significant log reduction in viable cells in monospecies biofilms (log CFU/mL) of C. albicans, S. aureus, and P. aeruginosa (0.636, 0.738, and 1.445, respectively) was detected after CAP exposure. Multispecies biofilms exposed to CAP showed significant viability reduction for C. albicans and S. aureus (1.385 and 0.831, respectively). The protocol was not cytotoxic to BEAS-2B. CAP can be a simple and effective method to delay multispecies biofilm formation inside the endotracheal prosthesis.

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“…However, in situ and even in vitro measurements of these components are often beyond the limits of current diagnostic tools. Several groups have attempted to investigate the penetration depth of CAP treatments in tissue models in hopes of further clarifying some of the biological effects that long-and short-lived species have [44,45].…”

Section: The Main Effectors Of Cap Treatmentmentioning

confidence: 99%

Cold Atmospheric Plasma Medicine: Applications, Challenges, and Opportunities for Predictive Control

Kazemi,

Nicol,

Bilén

et al. 2024

Plasma

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Plasma medicine is an emerging field that applies the science and engineering of physical plasma to biomedical applications. Low-temperature plasma, also known as cold plasma, is generated via the ionization of atoms in a gas, generally via exposure to strong electric fields, and consists of ions, free radicals, and molecules at varying energy states. Plasmas generated at low temperatures (approximately room temperature) have been used for applications in dermatology, oncology, and anti-microbial strategies. Despite current and ongoing clinical use, the exact mechanisms of action and the full range of effects of cold plasma treatment on cells are only just beginning to be understood. Direct and indirect effects of plasma on immune cells have the potential to be utilized for various applications such as immunomodulation, anti-infective therapies, and regulating inflammation. In this review, we combine diverse expertise in the fields of plasma chemistry, device design, and immunobiology to cover the history and current state of plasma medicine, basic plasma chemistry and their implications, the effects of cold atmospheric plasma on host cells with their potential immunological consequences, future directions, and the outlook and recommendations for plasma medicine.

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Penetration effect of the kINPen plasma jet investigated with a 3D agar-entrapped bacteria model

Cited by 6 publications

References 60 publications

Cold Atmospheric Helium Plasma in the Post-Covid Era: A Promising Tool for the Disinfection of Silicone Endotracheal Prostheses

Cold Atmospheric Helium Plasma in the Post-Covid Era: A Promising Tool for the Disinfection of Silicone Endotracheal Prostheses

Cold Atmospheric Helium Plasma in the Post-COVID-19 Era: A Promising Tool for the Disinfection of Silicone Endotracheal Prostheses

Cold Atmospheric Plasma Medicine: Applications, Challenges, and Opportunities for Predictive Control

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