Penetration in vitro of zona-free pig oocytes by homologous and heterologous spermatozoa

Zhao, Xian-Mian; Song, Xue-Xiong; Kawai, Yasuhiro; Niwa, K.

doi:10.1016/s0093-691x(02)00933-0

Cited by 16 publications

(12 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results were obtained with equine spermatozoa and bovine or hamster zona-free oocytes, with bovine spermatozoa and equine or porcine zona-free oocytes, and with rat spermatozoa and porcine zona-free oocytes [62][63][64][65]. Sartini and Berger [16] suggest that ligand-receptor conservation mediates the spermatozoa-oolemma binding between porcine and bovine species.…”

Section: Discussionsupporting

confidence: 70%

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Mugnier

Dell’Aquila

Peláez

et al. 2009

Biology of Reproduction

View full text Add to dashboard Cite

The mechanism of fertilization remains largely enigmatic in mammals. Most studies exploring the molecular mechanism underlying fertilization have been restricted to a single species, generally the mouse, without a comparative approach. However, the identification of divergences between species could allow us to highlight key components in the mechanism of fertilization. In the pig, in vitro fertilization (IVF) and polyspermy rates are high, and spermatozoa penetrate easily through the zona pellucida (ZP). In contrast, IVF rates are low in the horse, and polyspermy is scarce. Our objective was to develop a comparative strategy between these two divergent models. First, we compared the role of equine and porcine gametes in the following five functions using intraspecific and interspecific IVF: ZP binding, acrosome reaction, penetration through the ZP, gamete fusion, and pronucleus formation. Under in vitro conditions, we showed that the ZP is a determining element in sperm-ZP attachment and penetration, whereas the capacity of the spermatozoa is of less importance. In contrast, the capacity of the spermatozoa is a key component of the acrosome reaction step. Second, we compared the composition and structure of the equine and porcine ZP. We observed differences in the number and localization of the ZP glycoproteins and in the mesh-like structure of the ZP between equine and porcine species. These differences might correlate with the differences in spermatozoal attachment and penetration rates. In conclusion, our comparative approach allows us to identify determining elements in the mechanism of fertilization.

show abstract

Section: Discussionsupporting

confidence: 70%

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Mugnier

Dell’Aquila

Peláez

et al. 2009

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…It has been reported that the treatment of spermatozoa with caffeine results in increased in vitro penetration rates of bovine (Niwa & Ohgoda 1988) and porcine (Wang et al 1991, Zhao et al 2002 oocytes, and also results in a higher proportion of fertilisation (Parrish et al 1986, Niwa & Ohgoda 1988. However, the incidence of sperm penetration was either low or nil in mTBM medium without caffeine (Abeydeera & Day 1997b, Zhao et al 2002. ANP had a similar effect on the proportion of oocytes penetrated and fertilisation as that of caffeine, suggesting that ANP may play a physiological role in fertilisation.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, locally produced ANP may play an important role in mammalian sperm function. Although some agonists (such as caffeine and heparin) are widely used for spermatozoa capacitation and IVF in domestic animals (Parrish et al 1986, Niwa & Ohgoda 1988, Abeydeera & Day 1997b, Zhao et al 2002, the mechanisms of fertilisation in vivo are unclear. In the present study, we analyzed ANP and natriuretic peptide receptors gene expression in pig sperm and oviduct.…”

Section: Introductionmentioning

confidence: 99%

The expression of atrial natriuretic peptide in the oviduct and its functions in pig spermatozoa

Zhang

Hong

Zhou

et al. 2006

Journal of Endocrinology

View full text Add to dashboard Cite

Locally synthesized atrial natriuretic peptide (ANP) and its receptors have been found in reproductive tissues of various mammals, and play an important role in the acrosome reaction of human sperm. The objective of the present study was to examine the expression of ANP and its receptors in pig spermatozoa and oviduct, and the effect of ANP on pig spermatozoa function. The expression of ANP and its receptors was analyzed by RT-PCR. Only natriuretic peptide receptors-A (NPRA) mRNA was detected in fresh sperm. While the levels of natriuretic peptide receptors-C (NPRC) mRNA were low with no obvious change among different oviductal phases, the levels of ANP mRNA were high in oviduct(OT)1 , OT3 and OT5, but were very low in OT2. On the other hand, the levels of NPRA mRNA were low in OT1 and OT2, increased in OT3 and reached a maximum in OT4 and OT5. Western blot analysis revealed that the level of ANP was high in OT1, decreased in OT2 and OT3, and arrived at the nadir in OT4 and OT5. The effect of ANP on spermatozoa function was studied by the acrosome reaction and IVF. Incubation with ANP for 1 h significantly induced acrosome reaction of preincubated spermatozoa, and maximal response of acrosome reaction (34·1 2·3%) was achieved at 1 nM ANP treatment. Both C-ANP-(4-23), a selective ligand of NPRC, and caffeine had no effect on the acrosome reaction. The stimulatory effect of ANP on acrosome reaction could be mimicked by the permeable cGMP analog, 8-Br-cGMP. ANP and caffeine had a similar effect on improving the oocytes penetration rate, polyspermy rate and the average number of sperm per penetrated oocyte. Also, ANP treatment had a similar effect on cleavage rate, blastocyst formation rate and the number of cells per blastocyst as that of caffeine treatment. The effects of ANP on the acrosome reaction and the parameters of oocyte penetration could be blocked by cGMP-dependent protein kinase (PKG) inhibitors KT5823 and/or Rp-8-pCPT-cGMPS. These results suggest that the expression of ANP in the oviduct may be involved in the regulation of the acrosome reaction and the fertilising ability of pig spermatozoa, and the PKG pathway possibly participates in the process.

show abstract

“…In horses, conventional methods of semen quality evaluation, such as sperm motility, morphology, viability and concentration, are routinely used to rapidly estimate reproductive potential, but the results obtained do not correlate well with the final fertility rates achieved (Amann and Hammerstedt, 2002;Kuisma et al, 2006;Love, 2011;Rodríguez-Martínez, 2003). A better alternative is to examine the capacity of spermatozoa to penetrate homologous or heterologous oocytes in vitro (Cox et al, 1994;García-Álvarez et al, 2009;Macedo et al, 2006Macedo et al, , 2010Slavík and Fulka, 1992;Zhao et al, 2002). This has provided excellent results in donkeys (Taberner et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Pre-selection by double layer density gradient centrifugation improves the fertilising capacity of frozen–thawed, capacitated stallion sperm

Morató

Soares

Orero

et al. 2013

Animal Reproduction Science

View full text Add to dashboard Cite

Penetration in vitro of zona-free pig oocytes by homologous and heterologous spermatozoa

Cited by 16 publications

References 50 publications

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

The expression of atrial natriuretic peptide in the oviduct and its functions in pig spermatozoa

Pre-selection by double layer density gradient centrifugation improves the fertilising capacity of frozen–thawed, capacitated stallion sperm

Contact Info

Product

Resources

About