Penetration of gold nanoparticles across the stratum corneum layer of thick-Skin

Raju, Gayathri; Katiyar, Neeraj; Vadukumpully, Sajini; Shankarappa, Sahadev A.

doi:10.1016/j.jdermsci.2017.11.001

Cited by 59 publications

(34 citation statements)

References 38 publications

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“…This possibility and conditions for nanoparticles to penetrate skin layers and accumulate in certain compartments until recently are not fully studied. Size-dependent penetration of Au NP [58], including AuNSts [59], of different sizes into skin was studied using electron microscopy [58] and microscopic studies of haematoxylin and eosin stained skin sections [59]. Well detectability of Au nanoparticles with FLIM can be useful in further study of this issue.…”

Section: Resultsmentioning

confidence: 99%

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Perevedentseva

Ali

Lin

et al. 2020

Biomed. Opt. Express

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In the present work, we report the imaging of Au nanostars nanoparticles (AuNSt) and their multifunctional applications in biomedical research and theranostics applications. Their optical and spectroscopic properties are considered for the multimodal imaging purpose. The AuNSt are prepared by the seed-meditated method and characterized for use as an agent for bio-imaging. To demonstrate imaging with AuNSt, penetration and localization in different biological models such as cancer cell culture (A549 lung carcinoma cell), 3D tissue model (multicellular tumor spheroid on the base of human oral squamous carcinoma cell, SAS) and murine skin tissue are studied. AuNSt were visualized using fluorescence lifetime imaging (FLIM) at two-photon excitation with a pulse duration 140 fs, repetition rate 80 MHz and 780 nm wavelength femtosecond laser. Strong emission of AuNSt at two-photon excitation in the near infrared range and fluorescence lifetime less than 0.5 ns were observed. It allows using AuNSt as a fluorescent marker at two-photon fluorescence microscopy and lifetime imaging (FLIM). It was shown that AuNSt can be observed inside a thick sample (tissue and its model). This is the first demonstration using AuNSt as an imaging agent for FLIM at two-photon excitation in biosystems. Increased scattering of near-infrared light upon excitation of AuNSt surface plasmon oscillation was also observed and rendered using a possible contrast agent for optical coherence tomography (OCT). AuNSt detection in a biological system using FLIM is compared with OCT on the model of AuNSt penetrating into animal skin. The AuNSt application for multimodal imaging is discussed.

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Section: Resultsmentioning

confidence: 99%

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Perevedentseva

Ali

Lin

et al. 2020

Biomed. Opt. Express

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“…In general, researchers are interested in the influence on NP penetration routes and efficiency of the NP size, material, mechanical and surface properties (hydrophobicity/lipophilicity or hydrophilicity, charge, surface functionalization by molecular and ionic groups, as well as modification by conjugation with macromolecules) [5,7,8,36,42,80,81,82,83,84]. Among other things, the transfollicular pathway seems to be of special importance for NP penetration into intact healthy skin.…”

Section: Discussionmentioning

confidence: 99%

“…Note, that metallic NPs, particularly gold NPs, could be relevant both for intact and damaged skin [86]. The skin thickness (and accordingly the structure) can also be a significant parameter [84]. It has been shown using rabbit skin with a thinner stratum corneum (10–20 µm in thickness) compared to human or porcine skin that NPs of sizes 54–150 nm and different compositions can penetrate and distribute themselves through the skin.…”

Section: Discussionmentioning

confidence: 99%

Optical Studies of Nanodiamond-Tissue Interaction: Skin Penetration and Localization

et al. 2019

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In this work, several optical-spectroscopic methods have been used to visualize and investigate the penetration of diamond nanoparticles (NPs) of various sizes (3–150 nm), surface structures and fluorescence properties into the animal skin in vitro. Murine skin samples have been treated with nanodiamond (ND) water suspensions and studied using optical coherence tomography (OCT), confocal and two-photon fluorescence microscopy and fluorescence lifetime imaging (FLIM). An analysis of the optical properties of the used nanodiamonds (NDs) enables the selection of optimal optical methods or their combination for the study of nanodiamond–skin interaction. Among studied NDs, particles of 100 nm in nominal size were shown to be appropriate for multimodal imaging using all three methods. All the applied NDs were able to cross the skin barrier and penetrate the different layers of the epidermis to finally arrive in the hair follicle niches. The results suggest that NDs have the potential for multifunctional applications utilizing multimodal imaging.

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“…Iron-based nanoparticles (FeNP), gold nanoparticles (AuNP), palladium nanoparticles (PdNP), nickel nanoparticles (NiNP), AgNP, SiNP, and metal-based quantum dots (QD) have all been associated with penetration of the skin (Baroli et al 2007; Chu et al 2007; Filon et al 2011, 2016; Labouta et al 2011; Hirai et al 2012a; Rancan et al 2012; George et al 2014; Crosera et al 2016; Kraeling et al 2018). Moreover, many of these studies have established a relationship between decreased particle size and increased potential for skin permeation (Ryman-Rasmussen et al 2006; Sonavane et al 2008; Matsuo et al 2016; Raju et al 2018). Hydrophobicity, surface charge, and morphology are additional properties that have been shown to be influential in the capacity for these nanomaterials to pass through the stratum corneum (Rancan et al 2012; Lee et al 2013; Iannuccelli et al 2014; Fernandes et al 2015; Tak et al 2015; Mahmoud et al 2018).…”

Section: Metal Nanomaterials and Dermal Hypersensitivitymentioning

confidence: 99%

Metal nanomaterials: Immune effects and implications of physicochemical properties on sensitization, elicitation, and exacerbation of allergic disease

Roach

Stefaniak

Roberts

2019

Journal of Immunotoxicology

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The recent surge in incorporation of metallic and metal oxide nanomaterials into consumer products and their corresponding use in occupational settings have raised concerns over the potential for metals to induce size-specific adverse toxicological effects. Although nano-metals have been shown to induce greater lung injury and inflammation than their larger metal counterparts, their size-related effects on the immune system and allergic disease remain largely unknown. This knowledge gap is particularly concerning since metals are historically recognized as common inducers of allergic contact dermatitis, occupational asthma, and allergic adjuvancy. The investigation into the potential for adverse immune effects following exposure to metal nanomaterials is becoming an area of scientific interest since these characteristically lightweight materials are easily aerosolized and inhaled, and their small size may allow for penetration of the skin, which may promote unique size-specific immune effects with implications for allergic disease. Additionally, alterations in physicochemical properties of metals in the nano-scale greatly influence their interactions with components of biological systems, potentially leading to implications for inducing or exacerbating allergic disease. Although some research has been directed toward addressing these concerns, many aspects of metal nanomaterial-induced immune effects remain unclear. Overall, more scientific knowledge exists in regards to the potential for metal nanomaterials to exacerbate allergic disease than to their potential to induce allergic disease. Furthermore, effects of metal nanomaterial exposure on respiratory allergy have been more thoroughly-characterized than their potential influence on dermal allergy. Current knowledge regarding metal nanomaterials and their potential to induce/ exacerbate dermal and respiratory allergy are summarized in this review. In addition, an examination of several remaining knowledge gaps and considerations for future studies is provided.

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Penetration of gold nanoparticles across the stratum corneum layer of thick-Skin

Abstract: We conclude that the thick-skin allows nanoparticle penetration and acts as a depot for release of AuNPs into circulation long after the initial exposure has ceased.

Cited by 59 publications

References 38 publications

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems

Optical Studies of Nanodiamond-Tissue Interaction: Skin Penetration and Localization

Metal nanomaterials: Immune effects and implications of physicochemical properties on sensitization, elicitation, and exacerbation of allergic disease

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