Penetration of Human Eggs by Human Spermatozoa in vitro

McMaster, R.; Yanagimachi, Ryuzo; Lopata, Alexander

doi:10.1095/biolreprod19.1.212

Cited by 60 publications

(12 citation statements)

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“…Different families of ZP domain proteins assemble into similar supramolecular structures. Zona Pellucida fibers of mouse, hamster [24], rat [25], [26], [27], human [28], guinea pig [29] are of the same order – 10–20 nm as well as the size of filaments reconstructed from the purified proteins – 10–15 nm [23]. In case of medusa contact plate, we observed the filaments of the similar size collected in bundles (Fig.…”

Section: Discussionsupporting

confidence: 54%

Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

2012

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One of the A. aurita medusa main mesoglea polypeptides, mesoglein, has been described previously. Mesoglein belongs to ZP-domain protein family and therefore we focused on A.aurita oogenesis. Antibodies against mesoglein (AB RA47) stain the plate in the place where germinal epithelium contacts oocyte on the paraffin sections. According to its position, we named the structure found the “contact plate”. Our main instrument was AB against mesoglein. ZP-domain occupies about half of the whole amino acid sequence of the mesoglein. Immunoblot after SDS-PAGE and AU-PAGE reveals two charged and high Mr bands among the female gonad germinal epithelium polypeptides. One of the gonads' polypeptides Mr corresponds to that of mesogleal cells, the other ones' Mr is higher. The morphological description of contact plate formation is the subject of the current work. Two types of AB RA47 positive granules were observed during progressive oogenesis stages. Granules form the contact plate in mature oocyte. Contact plate of A.aurita oocyte marks its animal pole and resembles Zona Pellucida by the following features: (1) it attracts spermatozoids; (2) the material of the contact plate is synthesized by oocyte and stored in granules; (3) these granules and the contact plate itself contain ZP domain protein(s); (4) contact plate is an extracellular structure made up of fiber bundles similar to those of conventional Zona Pellucida.

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Section: Discussionsupporting

confidence: 54%

Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

2012

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“…The process of fertilization of human oocytes by spermatozoa in vitro has been described in detail by a number of workers Soupart & Strong, 1974McMaster, Yanagimachi & Lopata, 1978;Lopata, Sathananthan, McBain, Johnston & Speirs, 1980). The birth of normal babies following the transfer of 8-to 16-cell embryos to the uteri of women with blocked Fallopian tubes after fertilization in vitro (Edwards, Steptoe & Purdy, 1980b) would support the proposition that, at least in some cases, normal fertilization can occur in vitro.…”

Section: Introductionmentioning

confidence: 98%

Effect of delayed insemination on in-vitro fertilization, culture and transfer of human embryos

Trounson¹,

Mohr²,

Wood³

et al. 1982

Reproduction

346

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Summary. Oocytes were obtained from patients with tubal infertility at fixed times after the onset of the endogenous LH rise or hCG injection, and were inseminated immediately after recovery or after periods of 4\ p=n-\ 4\ m=1/ 2\ , 5\ p=n-\ 5\ m=1/ 2\ and 6\ p=n-\ 6\ m=1/ 2\ h in culture in vitro. Delayed insemination resulted in a marked increase in the proportion of oocytes that were fertilized and developed to normal embryos and maximum rates occurred after 5\ p=n-\ 5\ m=1/ 2\ h in culture ( 0\ p=n-\ \ m=1/ 2\ h, 26%; 4\ p=n-\ 4\ m=1/ 2\ h, 50%; 5\ p=n-\ 5\ m=1/ 2\ h, 89%; 6\ p=n-\ 6\ m=1/ 2\ h, 69%). The range and mean (\m=+-\s.d.) intervals from insemination for the pronuclear and early cleavage stages were 27\p=n-\43(35\m=.\6\m=+-\4\m=.\4)h for 2-cell stages, 36\p=n-\65 (45\m=.\7\ m=+-\ 8\m=.\3) h for 4-cell stages, 45\p=n-\73 (54\m=.\3\ m=+-\ 12\m=.\6) h for 8-cell stages and 68\p=n-\85 h for the 16-cell stage. In 7/50 patients receiving 1 or 2 embryos at the 2-, 4-and 8-cell stages, fetal development was normal and 2 women had twin pregnancies (36% success compared with 8% for single embryos). All pregnancies were from the groups in which insemination was delayed for 5\ p=n-\ 6\ m=1/ 2\ h. It is concluded that a short period of culture in vitro may allow the completion of oocyte maturation, and improve the results of in-vitro fertilization.

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“…Perreault & Rogers ( 1982) reported that the kinetics of capacitation may differ among individuals, but that the minimum capacitation time may be very short. Under appropriate conditions, less than 2 h may be sufficient for human sperm capacitation, the acrosome reaction, and subsequent zona pellucida penetration (McMaster et al, 1978;Overstreet et al, 1980). Regarding the kinetics of the AR, data obtained using rodent spermatozoa indicate that completion of the AR takes between 80 to 120 min on the zona surface (Florman & Storey, 1982;Bleil & Wassarman, 1983;Cherr et al, 1986;Yoshimatsu & Yanagimachi, 1988).…”

Section: Discussionmentioning

confidence: 99%

Sperm-oocyte interaction: studies on the kinetics of zona pellucida binding and acrosome reaction of human spermatozoa

et al. 2009

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Successful sperm-oocyte interaction depends, among other things, on sperm capacitation, which is defined by acrosomal and motility alterations. In the study described here the authors evaluated different aspects of this gamete interaction in humans. Specifically, the authors studied (1) the relationship between the number of spermatozoa bound to the zona pellucida and sperm concentration and incubation period, (2) the capacitation status and kinetics of acrosome reaction among the zona-bound spermatozoa, and (3) the effect of human follicular fluid on the zona-binding potential and acrosome status of spermatozoa from different men. The results indicated a concentration of lo7 cells ml-' after 15 min of coincubation to be the optimum for zona binding. The number of sperm bound after 0, 3 and 5 h of incubation was the same. In addition, spermatozoa incubated for 3 or 5 h underwent the acrosome reaction (range 9-43'10) on the zona surface within 15 min of binding. The maximum percentage of acrosome-reacted spermatozoa was reached after 60 min of binding. Follicular fluid affected the sperm populations selectively, since it did not influence zona binding capacity in all cases. The data enhances the authors' understanding of critical events occurring before fertilization.

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Penetration of Human Eggs by Human Spermatozoa in vitro

Cited by 60 publications

References 0 publications

Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

Aurelia aurita (Cnidaria) Oocytes' Contact Plate Structure and Development

Effect of delayed insemination on in-vitro fertilization, culture and transfer of human embryos

Sperm-oocyte interaction: studies on the kinetics of zona pellucida binding and acrosome reaction of human spermatozoa

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