Penetration of Human Ejaculated Spermatozoa into Human and Bovine Cervical Mucus. I. Correlation Between Penetration Values

Bergman, A; Amit, Ami; David, Menachem P.; Homonnai, Z. T.; Paz, Gedalia

doi:10.1016/s0015-0282(16)45739-0

Cited by 22 publications

(8 citation statements)

References 11 publications

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“…Semen was evaluated for volume, pH, and sperm quality. I n vitro sperm penetration test into precollected cervical mucus was performed using the scale of 1 to 4 (for poor to excellent), as described elsewhere (Bergman et al, 1981). The degree of motility was graded from 1 (poor) to 4 (excellent) forward motility.…”

Section: Methodsmentioning

confidence: 99%

Fertility of men following inguinal hernia repair

et al. 2009

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Among 8500 patients attending the fertility clinic due to infertility, 565 men (6.65%) reported an incidence of inguinal hernioplasty with or without subsequent atrophy of the testis. Semen quality (sperm concentrations, motility, and morphology) of these patients was markedly reduced in comparison to that of fertile men. In cases where hernioplasty was followed by atrophy of the testis, damage to sperm characteristics and Sertoli cell function was found to be far greater. This was also reflected in significant serum follicle stimulating hormone elevation ( P < 0.0025). No changes in luteinizing hormone and testosterone were found. No correlation was found between age of hernioplasty and semen quality following operation. The reasons for testicular damage may be due to ischaemic orchitis or immunological reactions.

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Section: Methodsmentioning

confidence: 99%

Fertility of men following inguinal hernia repair

et al. 2009

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“…In this analysis any variation due to mucus quality was eliminated by using a cryostored pool of bovine cervical mucus, since this material has been shown to reflect accurately the interaction of human spermatozoa and human mucus(Bergman, Amit, David, Homonnai & Paz, 1981;Mangione, Medley & Menge, 1981), exhibiting correlation coefficients of 0-982 and 0-931 in independent studies(Bergman et ai, 1981 ; R. J. Aitken, unpublished observations). In terms of the measurement of cervical mucus penetration, multiple regression equations incorporating the PSC scores as the dependent variable gave a poorer correlation with sperm movement than did Cm or Cm/Cs.…”

mentioning

confidence: 99%

Relationship between the movement characteristics of human spermatozoa and their ability to penetrate cervical mucus and zona-free hamster oocytes

Aitken

Sutton²,

Warner³

et al. 1985

Reproduction

195

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In a group of normospermic donors exhibiting hamster oocyte penetration scores of 0-100%, multiple regression analysis indicated that only 20% of the variation in fertilizing potential could be explained by differences in the movement characteristics of the spermatozoa following incubation in vitro. When the movement characteristics of the spermatozoa in semen were considered this figure was reduced to 6.8% as a result of significant differences in the motility patterns exhibited by the seminal and post-incubation sperm populations. A much closer relationship was observed between the movement characteristics of human spermatozoa in semen and their ability to penetrate cervical mucus. When differences in motile sperm densities were taken into account, 76% of the variation in cervical mucus penetration could be accounted for by the existence of linear correlations with certain aspects of sperm movement (multiple R = 0.874). Of the various attributes of sperm motility measured (linear velocity of progression, frequency of rotation, amplitude of sperm head displacement, % rolling and % yawing), a failure to exhibit an adequate amplitude of lateral sperm head displacement was consistently found to be the most significant factor determining the success of sperm-cervical mucus interaction (R2 = 0.53).

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“…The limiting factor of the performance of the in vitro penetration test is the availability of suitable mucus, especially in the cross-penetration tests. One solution to this prob lem was given by Bergman et al [1] who used bovine cervical mucus as a reference mucus. The characteristics of bovine and human cervical mucus were shown to be similar [4,11], The PV obtained in both the bovine mucus and human mucus were similar [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

“…One solution to this prob lem was given by Bergman et al [1] who used bovine cervical mucus as a reference mucus. The characteristics of bovine and human cervical mucus were shown to be similar [4,11], The PV obtained in both the bovine mucus and human mucus were similar [ 1 ]. Another solution to the problem is the development of a method to store the human cervical mucus.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Method for Preservation of Human Cervical Mucus for in vitro Penetration Tests

Baruch¹,

Yedwab²,

David³

et al. 1983

Gynecol Obstet Invest

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Midcycle cervical mucus was obtained from 56 women. Each mucus sample was divided into four portions collected into a capillary tube and kept either at room temperature or at 4°C, ––20°C or in liquid nitrogen for 1 h, afterwards thawed at 37°C and used in the in vitro penetration device for 1 h. 56 semen samples of good quality were used, the same sample for all four types of the same cervical mucus. Very high significant correlations were calculated between the penetration value (PV) rates in all four methods of cervical mucus preservation. Paired t test revealed a significant decrease (p < 0.025) in the PV rates of sperm in the cervical mucus cryopreserved in liquid nitrogen. The results of this study recommend the establishment of a cervical mucus bank using the 4CC and ––20°C as possible methods for preservation of cervical mucus.

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Penetration of Human Ejaculated Spermatozoa into Human and Bovine Cervical Mucus. I. Correlation Between Penetration Values

Cited by 22 publications

References 11 publications

Fertility of men following inguinal hernia repair

Fertility of men following inguinal hernia repair

Relationship between the movement characteristics of human spermatozoa and their ability to penetrate cervical mucus and zona-free hamster oocytes

Establishment of a Method for Preservation of Human Cervical Mucus for in vitro Penetration Tests

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