Penetration of normal, damaged and diseased skin — An in vitro study on dendritic core–multishell nanotransporters

Alnasif, Nesrin; Zoschke, Christian; Fleige, Emanuel; Brodwolf, Robert; Boreham, Alexander; Rühl, E.; Eckl, Katja Martina; Merk, Hans F.; Hennies, Hans Christian; Alexiev, Ulrike; Haag, Rainer; Küchler, Sarah; Schäfer‐Korting, Monika

doi:10.1016/j.jconrel.2014.04.006

Cited by 79 publications

(80 citation statements)

References 31 publications

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“…Additionally, we studied the toxicity and biocompatibility of non-loaded PG-PEG particles employing different in vitro setups. Knowledge about the toxicity of locally applied carrier systems is of great importance, particularly in diseased or barrier impaired skin, because the nanoparticles can penetrate into deeper dermal layers and be taken up into the systemic circulation [14,18]. In contrast to CMS nanotransporters, PG-PEG particles are less structurally defined and the alkyl and PEG chains are randomly attached to the dendritic PG core without a multishell formation [22].…”

Section: Resultsmentioning

confidence: 99%

“…One possibility is that the particles themselves overcome the SC and co-transport the loaded compounds into deeper dermal layers. However, recent studies of our group clearly showed that after 6 h CMS nanotransporters did not penetrate into viable skin layers but accumulated in the SC [18]. In general, the magnitude of skin absorption of nanoparticles is still the subject of ongoing research with often contradictory results.…”

Section: Dermal Drug Deliverymentioning

confidence: 94%

“…Findings which underline this hypothesis have been made by our group using fluorescence lifetime imaging microscopy (FLIM). FLIM revealed intense interactions between CMS nanotransporters and the SC which was not observed in tape stripped skin [18]. Currently, we are evaluating in detail the interactions of polymeric carrier systems with the skin lipids and proteins in order to unravel the mechanism of enhanced drug delivery.…”

Section: Dermal Drug Deliverymentioning

confidence: 99%

“…However, evidence has emerged that nanoparticle penetration into viable layers of intact human skin is very limited [15,16]. Nevertheless, hyperbranched dendritic core-multishell (CMS) nanotransporters which are composed of a dendritic PG core surrounded by an internal C18 alkyl shell and an outermost methoxy polyethylene glycol (mPEG) shell [17] overcame the SC after a prolonged contact time of 24 h [18]. Biocompatibility studies showed no toxic effects as well as no local irritation following the topical application of CMS nanotransporters [19].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Impact of structural differences in hyperbranched polyglycerol–polyethylene glycol nanoparticles on dermal drug delivery and biocompatibility

Kumar

Alnasif

Fleige

et al. 2014

European Journal of Pharmaceutics and Biopharmaceutics

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Section: Resultsmentioning

confidence: 99%

Section: Dermal Drug Deliverymentioning

confidence: 94%

Section: Dermal Drug Deliverymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of structural differences in hyperbranched polyglycerol–polyethylene glycol nanoparticles on dermal drug delivery and biocompatibility

Kumar

Alnasif

Fleige

et al. 2014

European Journal of Pharmaceutics and Biopharmaceutics

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“…Nanomaterials provide attractive options to resolve this problem. Various technologies using nanosized and microsized carriers have been developed to increase the low rate of penetration of active agents through the skin (5,6). Hyperthermia is a therapeutic procedure that increases tissue temperatures by using physical methods, such as microwave, radiofrequency, laser, and ultrasound.…”

mentioning

confidence: 99%

Killing of Staphylococcus aureus via Magnetic Hyperthermia Mediated by Magnetotactic Bacteria

Chen

Yao

et al. 2016

Appl Environ Microbiol

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e Staphylococcus aureus is a common hospital and household pathogen. Given the emergence of antibiotic-resistant derivatives of this pathogen resulting from the use of antibiotics as general treatment, development of alternative therapeutic strategies is urgently needed. Here, we assess the feasibility of killing S. aureus cells in vitro and in vivo through magnetic hyperthermia mediated by magnetotactic bacteria that possess magnetic nanocrystals and demonstrate magnetically steered swimming. The S. aureus suspension was added to magnetotactic MO-1 bacteria either directly or after coating with anti-MO-1 polyclonal antibodies. The suspensions were then subjected to an alternating magnetic field (AMF) for 1 h. S. aureus viability was subsequently assessed through conventional plate counting and flow cytometry. We found that approximately 30% of the S. aureus cells mixed with uncoated MO-1 cells were killed after AMF treatment. Moreover, attachment between the magnetotactic bacteria and S. aureus increased the killing efficiency of hyperthermia to more than 50%. Using mouse models, we demonstrated that magnetic hyperthermia mediated by antibody-coated magnetotactic MO-1 bacteria significantly improved wound healing. These results collectively demonstrated the effective eradication of S. aureus both in vitro and in vivo, indicating the potential of magnetotactic bacterium-mediated magnetic hyperthermia as a treatment for S. aureus-induced skin or wound infections. Staphylococcus aureus is a major pathogen that produces toxins and superantigens causing skin and soft tissue infections in hospitals or communities (1-3). Antibiotic therapy is not highly efficient because the routine and intensive use of antibiotics has caused the emergence of both hospital-and community-associated methicillin-resistant S. aureus. Moreover, S. aureus tends to form biofilms that reduce the susceptibility of the pathogen to the immune system and topical antimicrobials, rendering treatment of infections using antibiotics less effective (4). The need to develop alternative therapeutic treatments against S. aureus to address this major public health problem is urgent. Nanomaterials provide attractive options to resolve this problem. Various technologies using nanosized and microsized carriers have been developed to increase the low rate of penetration of active agents through the skin (5, 6). Hyperthermia is a therapeutic procedure that increases tissue temperatures by using physical methods, such as microwave, radiofrequency, laser, and ultrasound. However, a bottleneck for the clinical application of hyperthermia is the difficulty of controlling the temperature temporally and spatially, particularly in deep body regions. Magnetic hyperthermia offers an attractive solution to this problem. Upon exposure to an alternating magnetic field, magnetic nanoparticles (MNPs) produce heat mainly on the basis of the mechanisms of hysteresis losses (7), relaxation losses (Néel or Brown relaxation) (8-10), and eddy current effect (11). Magnetic hyperth...

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Overcoming the Translational Gap – Nanotechnology in Dermal Drug Delivery

Zoschke¹,

Schäfer‐Korting²

2021

Fundamentals of Drug Delivery

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Penetration of normal, damaged and diseased skin — An in vitro study on dendritic core–multishell nanotransporters

Cited by 79 publications

References 31 publications

Impact of structural differences in hyperbranched polyglycerol–polyethylene glycol nanoparticles on dermal drug delivery and biocompatibility

Impact of structural differences in hyperbranched polyglycerol–polyethylene glycol nanoparticles on dermal drug delivery and biocompatibility

Killing of Staphylococcus aureus via Magnetic Hyperthermia Mediated by Magnetotactic Bacteria

Overcoming the Translational Gap – Nanotechnology in Dermal Drug Delivery

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