Penetration of UV-A, UV-B, blue, and red light into leaf tissues of pecan measured by a fiber optic microprobe system

Qi, Yadong; Bai, Shuju; Vogelmann, Thomas C.; Heisler, Gordon M.

doi:10.1117/12.506629

Cited by 40 publications

(8 citation statements)

References 4 publications

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“…Moreover, since HY5 and HYH also work downstream of photoreceptors that perceive wavelengths longer than UV-B wavelength, similar mechanisms to drive phenotypic outputs may be expected at other wavelengths. This will of course depend on the optical properties of the tissues, and differential responses may be less pronounced for UV-B light, which penetrates less deeply into plant tissues compared with longer wavelengths (Markstädter et al, 2001;Qi et al, 2003).…”

Section: Different Molecular Mechanisms Involved In the Phototropism mentioning

confidence: 99%

Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism

et al. 2019

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In the course of evolution, plants have developed mechanisms that orient their organs toward the incoming light. At the seedling stage, positive phototropism is mainly regulated by phototropin photoreceptors in blue and UV wavelengths. Contrasting with this, we report that UV RESISTANCE LOCUS8 (UVR8) serves as the predominant photoreceptor of UV-B-induced phototropic responses in Arabidopsis (Arabidopsis thaliana) inflorescence stems. We examined the molecular mechanisms underlying this response and our findings support the Blaauw theory (Blaauw, 1919), suggesting rapid differential growth through unilateral photomorphogenic growth inhibition. UVR8-dependent UV-B light perception occurs mainly in the epidermis and cortex, but deeper tissues such as endodermis can also contribute. Within stems, a spatial difference of UVR8 signal causes a transcript and protein increase of transcription factors ELONGATED HYPOCOTYL5 (HY5) and its homolog HY5 HOMOLOG at the UV-B-exposed side. The irradiated side shows (1) strong activation of flavonoid synthesis genes and flavonoid accumulation; (2) increased gibberellin (GA)2-oxidase expression, diminished GA1 levels, and accumulation of the DELLA protein REPRESSOR OF GA1; and (3) increased expression of the auxin transport regulator PINOID, contributing to diminished auxin signaling. Together, the data suggest a mechanism of phototropin-independent inflorescence phototropism through multiple, locally UVR8-regulated hormone pathways.

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Section: Different Molecular Mechanisms Involved In the Phototropism mentioning

confidence: 99%

Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism

et al. 2019

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“…UV‐B penetrates rather poorly into tissues below the epidermis. Indeed, leaf epidermal transmittance of UV‐B is less than 10%, measured in many different species under various circumstances (Robberecht et al ., ; Day et al ., ; Markstadter et al ., ; Qi et al ., ; Nybakken et al ., ). UVR8 is expressed ubiquitously in different organs of mature Arabidopsis (Rizzini et al ., ), but the precise distribution pattern and the accumulation level of the photoreceptor in various tissues have not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Expression of the UVR8 photoreceptor in different tissues reveals tissue‐autonomous features of UV‐B signalling

Bernula

Crocco

Arongaus

et al. 2017

Plant Cell & Environment

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The Arabidopsis UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) orchestrates the expression of hundreds of genes, many of which can be associated with UV-B tolerance. UV-B does not efficiently penetrate into tissues, yet UV-B regulates complex growth and developmental responses. To unravel to what extent and how UVR8 located in different tissues contributes to UV-B-induced responses, we expressed UVR8 fused to the YELLOW FLUORESCENT PROTEIN (YFP) under the control of tissue-specific promoters in a uvr8 null mutant background. We show that (1) UVR8 localized in the epidermis plays a major role in regulating cotyledon expansion, and (2) expression of UVR8 in the mesophyll is important to protect adult plants from the damaging effects of UV-B. We found that UV-B induces transcription of selected genes, including the key transcriptional regulator ELONGATED HYPOCOTYL 5 (HY5), only in tissues that express UVR8. Thus, we suggest that tissue-autonomous and simultaneous UVR8 signalling in different tissues mediates, at least partly, developmental and defence responses to UV-B.

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“…In a few cases, the responses of a photosynthetic tissue (e.g. a leaf) to different colours of light (blue, green and red) have been compared to evince the effect of different light penetrations on photosynthesis (Qi et al, 2003; Rappaport et al, 2007; Terashima et al, 2009). Both approaches have limitations as they do not rely on direct assessment of photosynthesis inside an intact photosynthetic tissue/organ.…”

Section: Introductionmentioning

confidence: 99%

Tailoring confocal microscopy for in-cell photophysiology studies

Storti

Hsine

Uwizeye

et al. 2022

Preprint

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Photoautotrophs environmental responses have been extensively studied at the organism and ecosystem level. However, less is known about their photosynthesis at the single cell level. This information is needed to understand photosynthetic acclimation processes, as light changes as it penetrates cells, layers of cells or organs. Furthermore, cells within the same tissue may behave differently, being at different developmental/physiological stages. Here we describe a new approach for single-cell and subcellular photophysiology based on the customisation of confocal microscopy to assess chlorophyll fluorescence quenching by the saturation pulse method. We exploit this setup to: i. reassess the specialisation of photosynthetic activities in developing tissues of non-vascular plants; ii. identify a specific subpopulation of phytoplankton cells in marine photosymbiosis, which are consolidating metabolic connections with their animal hosts, and iii. testify to the link between light penetration and photoprotection responses inside the different tissues that constitute a plant leaf anatomy.

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Penetration of UV-A, UV-B, blue, and red light into leaf tissues of pecan measured by a fiber optic microprobe system

Cited by 40 publications

References 4 publications

Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism

Differential UVR8 Signal across the Stem Controls UV-B–Induced Inflorescence Phototropism

Expression of the UVR8 photoreceptor in different tissues reveals tissue‐autonomous features of UV‐B signalling

Tailoring confocal microscopy for in-cell photophysiology studies

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