Penetration studies of topically applied substances: optical determination of the amount of stratum corneum removed by tape stripping

Lademann, Jürgen; Ilgevičius, A.; Zurbau, O.; Liess, H.-D.; Schanzer, Sabine; Weigmann, H.‐J.; Antoniou, C.; Pelchrzim, R. von; Sterry, Wolfram

doi:10.1117/1.2359466

Cited by 34 publications

(15 citation statements)

References 19 publications

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“…Since a large discrepancy of skin penetration existed among the various buffers, the partitioning or permeation process via skin but not the release process was the rate-limiting step for Se-L-M. Tape stripping is a simple standard technique for evaluating the function of the SC in percutaneous absorption studies [33] . Se-L-M in pH 4 buffer showed the highest enhancement of skin permeation across SC-stripped skin compared to the other buffer systems, suggesting that diffusion through the SC layer was important for permeation in a cationic form.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Lin

Fang²,

Al‐Suwayeh

et al. 2011

Acta Pharmacol Sin

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Aim: To investigate the in vitro and in vivo percutaneous absorption of seleno-L-methionine (Se-L-M), an ultraviolet (UV)-protecting agent, from aqueous solutions. Methods: Aqueous solutions of Se-L-M were prepared in pH 4, 8, and 10.8 buffers. The pH 8 buffer contained 30% glycerol, propylene glycol (PG) and polyethylene glycol (PEG) 400. The in vitro skin permeation of Se-L-M via porcine skin and nude mouse skin was measured and compared using Franz diffusion cells. The in vivo skin tolerance study was performed, which examined transepidermal water loss (TEWL), skin pH and erythema. Results: In the excised porcine skin, the flux was 0.1, 11.4 and 8.2 μg· cm -2 ·h -1 for the pH 4, 8, and 10.8 buffers, respectively. A linear correlation between the flux and skin deposition was determined. According to permeation across skin with different treatments (stripping, delipidation, and ethanol treatments), it was determined that the intracellular route comprised the predominant pathway for Se-L-M permeation from pH 8 buffer. Aqueous solutions of seleno-DL-methionine (Se-DL-M), selenium sulfide and selenium-containing quantum dot nanoparticles were also used as donor systems. The DL form showed a lower flux (7.0 vs 11.4 μg· cm -2 ·h -1 ) and skin uptake (23.4 vs 47.3 μg/g) as compared to the L form, indicating stereoselective permeation of this compound. There was no or only negligible permeation of selenium sulfide and quantum dots into and across the skin. With in vivo topical application for 4 and 8 h, the skin deposition of Se-L-M was about 7 μg/g, and values were comparable to each other. The topical application of Se-L-M for up to 5 d did not caused apparent skin irritation. However, slight inflammation of the dermis was noted according to the histopathological examination. Conclusion: Se-L-M was readily absorbed by the skin in both the in vitro and in vivo experiments. The established profiles of Se-L-M skin absorption will be helpful in developing topical products of this compound.Keywords: seleno-L-methionine; selenium; percutaneous absorption; topical delivery; skin [5] . In humans, a low selenium status is associated with up to a 4-fold increased risk of developing skin cancer [6] . Topical selenium compounds also increase the minimum dose of UV radiation required to cause skin reddening and protect against skin damage caused by UVB [7,8] . Seleno-L-methionine (Se-L-M), the major component of dietary selenium, represents an organic form of selenium which may provide enhanced protection of the skin [9] . Se-L-M also decreases the oxidative stress of neurodegenerative diseases, rheumatoid arthritis, and HIV/AIDS [10,11] . Most ingested selenium, whether in organic or inorganic form, is converted by liver metabolism [3] . In addition, selenium toxicity can produce gastrointestinal problems [12] . Delivery of pharmacologically active compounds via the skin is an attractive alternative to oral dosing for numerous reasons including stable plasma concentrations, bypass of firstpass effects, and reduction of some si...

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Lin

Fang²,

Al‐Suwayeh

et al. 2011

Acta Pharmacol Sin

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“…Tape stripping was performed 10 min after application and pH SC was measured after every 10 tape strippings. In total, 100 tape strippings were performed to remove the complete SC [16,17]. Changes in pH SS over time were measured without removal of the SC on the other arm.…”

Section: Methodsmentioning

confidence: 99%

Impact of a Glycolic Acid-Containing pH 4 Water-in-Oil Emulsion on Skin pH

Behm

Kemper

Babilas

et al. 2015

Skin Pharmacol Physiol

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The skin pH is crucial for physiological skin functions. A decline in stratum corneum acidity, as observed in aged or diseased skin, may negatively affect physiological skin functions. Therefore, glycolic acid-containing water-in-oil (W/O) emulsions adjusted to pH 4 were investigated regarding their effect on normal or increased skin pH. A pH 4 W/O emulsion was applied on three areas with pathologically increased skin surface pH in diabetics (n = 10). Further, a 28-day half-side trial (n = 30) was performed to test the long-term efficacy and safety of a pH 4 W/O emulsion (n = 30). In summary, the application of a pH 4 W/O emulsion reduced the skin pH in healthy, elderly and diabetic subjects, which may improve epidermal barrier functions.

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“…Tape stripping removed SC partially or totally, and it is a simple standard technique for the establishment of skin barrier-damaged model. 30 However, NP penetration through tape stripped skin did not always lead to marked penetration enhancement. It varied qualitatively in magnitude from none [31][32][33][34] to some NPs detected.…”

mentioning

confidence: 99%

“…29,30 To investigate the permeability of NPs in barrier-damaged skin, we tape stripped samples mimicking barrier disruption. Tape stripping removed SC partially or totally, and it is a simple standard technique for the establishment of skin barrier-damaged model.…”

mentioning

confidence: 99%

Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro

Zou¹,

Celli²,

Zhu³

et al. 2017

IJN

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Objective With rapid development of nanotechnology, there is increasing interest in nanoparticle (NP) application and its safety and efficacy on human skin. In this study, we utilized confocal laser scanning microscopy to estimate NP skin penetration. Methods Three different-sized polystyrene NPs marked with red fluorescence were applied to human skin, and Calcium Green 5N was used as a counterstain. Dimethyl sulfoxide (DMSO) and ethanol were used as alternative vehicles for NPs. Tape stripping was utilized as a barrier-damaged skin model. Skin biopsies dosed with NPs were incubated at 4°C or 37°C for 24 hours and imaged using confocal laser scanning microscopy. Results NPs were localized in the stratum corneum (SC) and hair follicles without penetrating the epidermis/dermis. Barrier alteration with tape stripping and change in incubation temperature did not induce deeper penetration. DMSO enhanced NP SC penetration but ethanol did not. Conclusion Except with DMSO vehicle, these hydrolyzed polystyrene NPs did not penetrate intact or barrier-damaged human “viable” epidermis. For further clinical relevance, in vivo human skin studies and more sensitive analytic chemical methodology are suggested.

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Penetration studies of topically applied substances: optical determination of the amount of stratum corneum removed by tape stripping

Cited by 34 publications

References 19 publications

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Impact of a Glycolic Acid-Containing pH 4 Water-in-Oil Emulsion on Skin pH

Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro

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Penetration studies of topically applied substances: optical determination of the amount of stratum corneum removed by tape stripping

Cited by 34 publications

References 19 publications

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

In vitro and in vivo percutaneous absorption of seleno-L-methionine, an antioxidant agent, and other selenium species

Impact of a Glycolic Acid-Containing pH 4 Water-in-Oil Emulsion on Skin pH

Confocal laser scanning microscopy to estimate nanoparticles&rsquo; human skin penetration in vitro

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Product

Resources

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Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro