Human activity causes water pollution in Situ Cikaret and Situ Cilodong, in Bogor and Depok, West Java, respectively. The pollutant and toxicant altered the behaviour, anatomy, and physiology of fish in those habitats. This study aimed to analyze the histopathological features of fish gills in Situ Cikaret and Situ Cilodong. The physical and chemical parameters, including pH, temperature, and dissolved oxygen (DO), were measured. Four fish species, Spotted barb fish (Barbodes binotatus), Amazon sailfin catfish (Pterygoplichthys pardalis), Yellow rasbora fish (Rasbora lateristriata), and Louhan fish (Amphilopus citrinellus) were collected. The gills were processed with paraffin, cut into 5 μm, and stained with hematoxylin-eosin. The histological features of Amazon sailfin catfish showed a normal feature, whilst the others showed an abnormality, including hyperplasia, leucocyte proliferation, secondary lamella fusion, vasodilatation, oedema, necrosis, and hypertropia mucus cells, and chloride cells. Although the temperatures, pH, and DO in this study were within the normal values, the abnormality of histological features indicated that Situ Cikaret and Situ Cilodong were polluted.