Pengaruh Mutasi Fisik Iradiasi Sinar Gamma terhadap Keragaman Genetik dan Penampilan Coleus blumei

Togatorop, Eny Rolenti; Aisyah, Syarifah Iis; Damanik, Muhammad Rizal Martua

doi:10.29244/jhi.7.3.187-194

Cited by 5 publications

(2 citation statements)

References 3 publications

(3 reference statements)

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“…These differences in the plants' characters grown in a uniform environmental condition can be attributed to genetic differences that have changed due to the gamma-ray mutagens. Mutations can be artificially induced with physical mutagens through gamma-ray irradiation [12]. The Probit analysis showed a linear equation between irradiation dose and seed mortality (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Initial vegetative growth and determination of the lethal dose of local rice arias putih at various doses of gamma irradiation

Siregar,

Rosmayati,

Hanafiah

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Local rice cultivars that are resistant to biotic and abiotic stress, in fact, still have weaknesses in terms of longevity and idiotypes that causes farmers to rarely cultivate local rice cultivars commercially. One method of plant breeding that can be taken to correct deficiencies in a plant is a mutation program using gamma-ray irradiation. The use of gamma rays must be tested for the value of LD50 so that the right dose is obtained in an effort to increase diversity. One of the upland rice varieties, Arias Putih, was irradiated with gamma rays at the following doses, G0=0 Gy, G1=100 Gy, G2=200 Gy, G3=300 Gy, G4=400 Gy, G5=500 Gy, G6=600 Gy, G7=700 Gy, G8=800 Gy, G9=900 Gy, G10=1000 Gy, and G11=1100 Gy. This LD 50 was carried out with three repetitions per treatment dose, including control. Each dose of gamma-ray irradiation used 100 rice seeds and sown in the seedbed for three weeks. The parameters observed were germination percentage, seedling height and root length, crown and root fresh weight. LD50 was obtained from germination percentage data and was analyzed using Curve-Fit Analysis software. The results revealed a decrease in growth character as the irradiation dose increased. Increasing the irradiation dose up to 500 Gy caused the seeds to die. The LD50 value obtained from the study was 224.13 Gy.

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Section: Resultsmentioning

confidence: 99%

Initial vegetative growth and determination of the lethal dose of local rice arias putih at various doses of gamma irradiation

Siregar,

Rosmayati,

Hanafiah

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…Teknik iradiasi sinar gamma, sinar radioaktif maupun sinar X dapat memperbaiki sifat suatu varietas tanaman tanpa mengubah sifat lainnya melalui mutasi, dan menghasilkan sifat baru yang tidak dimiliki oleh tanaman induknya (Sari et al 2018). Iradiasi sinar gamma merupakan teknik mutasi tanaman untuk menghasilkan keragaman genetik sebagai bahan materi variasi unggul baru (Togatorop et al 2016). Iradiasi sinar gamma dapat meningkatkan ketahanan tanaman terhadap gangguan penyakit maupun kekeringan (Iwo et al 2013).…”

Section: Pendahuluanunclassified

The The Effect of Gamma Rays on Local Chilli Infected to Pepper Yellow Leaf Curl Virus Infection (Begomovirus)

Tarigan¹,

Hanafia

Sinuraya

et al. 2023

J Fitopatol Indones

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Cabai merupakan salah satu komoditas hortikultura bernilai ekonomi tinggi di Indonesia. Cabai lokal Karo berbatang ungu merupakan cabai yang memiliki keunggulan produksi dan pertumbuhan vegetatif tinggi, namun sangat rentan terhadap penyakit virus keriting kuning oleh begomovirus. Salah satu upaya pengendalian begomovirus ialah dengan perakitan genotipe baru yang tahan melalui induksi iradiasi sinar gamma. Penelitian bertujuan mengkaji pengaruh iradiasi sinar gamma pada benih cabai lokal Karo berbatang ungu terinfeksi begomovirus untuk mendapatkan calon genotipe mutan yang tahan. Benih dari tanaman cabai terinfeksi begomovirus diberi perlakuan iradiasi sinar gamma dengan taraf dosis 150, 200, dan 250 Gy untuk mendapatkan tanaman mutan 1 (M1). Benih sehat dan benih berasal dari tanaman sakit tanpa perlakuan digunakan sebagai tanaman kontrol. Parameter yang diamati meliputi insidensi dan keparahan penyakit, AUDPC dan deteksi begomovirus pada tanaman M1 dan benih generasi kedua (M2). Perlakuan iradiasi sinar gamma taraf dosis 150 Gy pada benih cabai terinfeksi begomovirus menunjukkan rata-rata insidensi, keparahan penyakit, dan AUDPC paling rendah dibandingkan dengan perlakuan lainnya pada tanaman mutan M1 dan M2. Berdasarkan deteksi dengan PCR pada tanaman mutan M2, dari perlakuan dosis 150 Gy didapatkan 7 calon genotipe tahan begomovirus terbanyak di antara perlakuan lainnya.

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Morphological characterization and gamma irradiation effect on plant growth of Curcuma heyneana Val & Zijp

Hapsari

Trimanto

Isnaini

et al. 2021

INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020)

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Pengaruh Mutasi Fisik Iradiasi Sinar Gamma terhadap Keragaman Genetik dan Penampilan Coleus blumei

Cited by 5 publications

References 3 publications

Initial vegetative growth and determination of the lethal dose of local rice arias putih at various doses of gamma irradiation

Initial vegetative growth and determination of the lethal dose of local rice arias putih at various doses of gamma irradiation

The The Effect of Gamma Rays on Local Chilli Infected to Pepper Yellow Leaf Curl Virus Infection (Begomovirus)

Morphological characterization and gamma irradiation effect on plant growth of Curcuma heyneana Val & Zijp

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