Pengaruh NAA dan BAP terhadap Kultur Jaringan Nenas Tangkit (Ananas comosus (L.) Merr. cv. Tangkit)

Zulkarnain, Zulkarnain; Neliyati, Neliyati

doi:10.22437/biospecies.v10i1.3480

Cited by 9 publications

(10 citation statements)

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“…Ikatan rangkap tersebut efektif untuk memacu pertumbuhan tunas dan daun pada eksplan in vitro. Zulkarnain (2009) juga menyatakan bahwa perimbangan konsentrasi auksin dan sitokinin eksogen dengan fitohormon endogen menyebabkan proses fisiologis pada eksplan berlangsung optimal dalam memicu awal pertumbuhan tunas. Setiap sel memiliki respon masing-masing terhadap zat pengatur tumbuh yang diberikan.…”

Section: Pendahuluanunclassified

Optimasi Induksi Tunas Aksiler Dendrobium nobile Melalui Kombinasi 2-iP dan 2,4-D In Vitro

Milah,

Sugiyarto,

Ratnawati

et al. 2023

AGROISTA

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This research aims to determine optimum combination of addition 2-iP and 2,4-D with different concentration and the nodus position for induction Dendrobium Red Emperor ‘Prince’ axial shoots. The research employed a CFR design with two factors, namely: variation of combined concentration 2-iP and 2,4-D (A: 1 ppm 2-iP + 2 ppm 2,4-D; B: 2 ppm 2-iP + 1,5 ppm 2,4-D; C: 3 ppm 2-iP + 1 ppm 2,4-D; D: 4 ppm 2-iP + 0,5 ppm 2,4-D) each treatment was repeated 6 times and the nodal position (top, middle, bottom). The basic medium is NP+CW. The axillary bud growth is measured based on the time of bud emerge, shoot growth, explant percentage which form axillary bud, root growth, and plantlet wet weight. Data obtained were analyzed using ANOVA and continued with DMRT 5%. Results show that B treatment give fastest bud emerging time, and highest stem diameter, buds height, leaves length, leaves width, roots length. A treatment shows best results on shoots number, plantlet wet weight, number of leaves, and number of roots. The optimum combined concentration of 2-iP and 2,4-D to induce axillary shoots is 2 ppm 2-iP + 1,5 ppm 2,4-D, whilst best nodus position is the top one.

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Section: Pendahuluanunclassified

Optimasi Induksi Tunas Aksiler Dendrobium nobile Melalui Kombinasi 2-iP dan 2,4-D In Vitro

Milah,

Sugiyarto,

Ratnawati

et al. 2023

AGROISTA

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“…The concentration of sucrose contained in the media had an effect on the growth of the number of roots. The addition of inappropriate PGR tends to cause inhibition of morphogenesis in plants [1]. According to [19] which stated that the results of the analysis on chrysanthemum plants showed that tomato extract also affected the speed of plant root growth, but the control treatment (no addition of tomato extract) gave better root length.…”

Section: Number Of Rootsmentioning

confidence: 99%

“…Tissue culture is a method for separating plant components like cells, tissues, organs and seeds which are then propagated on sterile artificial media so that the plant parts are able to regenerate and differentiate into complete plants [1]. The use of tissue culture is to obtain new plants in large quantities and in a relatively short time and have the exact same physiological and morphological properties as the parent.…”

Section: Introductionmentioning

confidence: 99%

Effect of tomato (Solanum lycopersicum) extract on height and number of roots in patchouli (Pogostemon cablin Benth.) subculture planlet

Yunita,

Izzah,

Amalia

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Extracts of plant parts have the potential as growth regulators that can be used in in vitro culture. In order to find the ideal tomato extract concentration for the growth of patchouli explan, this study examine the effect of tomato extract (Solanum lycopersicum) as natural growth regulator on the height and quantity of roots in patchouli (Pogotemon cablin Benth.) subculture. This research was conducted from March to May 2022 at the Laboratory of Plant Physiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University. This study used a completely randomized design (CRD) with 6 treatments and 5 replications. The treatments consisted of P0 (MS medium 0), P1 (MS medium + 4 ml/L tomato fruit extract), P2 (MS medium + 6 ml/L tomato fruit extract), P3 (MS medium + 8 ml/L tomato fruit extract), P4 (MS medium + 10 ml/L tomato fruit extract) and P5 (MS medium + 12 ml/L tomato extract). Analysis of Variance (ANOVA) and continued by Duncan post-hoc test at a significant level of 95% applied to data by using SPSS version 18. The outcomes demonstrated that the injection of tomato fruit extract had a substantial impact on the roots rather than the height parameter plantlets. The control treatment (MS 0) resulted in the best average number of roots, it was suspected that the tomato fruit extract contained compounds which inhibited root growth.

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“…Meskipun NAA banyak dilaporkan berperan dalam menginduksi akar (Sesay et al, 2018;Sukmadjaja & Widhiastuti, 2011;Yirssaw et al, 2014), namun dalam penelitian ini kombinasi NAA dengan konsentrasi yang rendah yaitu 0,05 mg L -1 dan BA 0,4 mg L -1 dapat meningkatkan jumlah tunas pada klon Unila UK-1 (Tabel 2) dan jumlah buku pada BW-1 (Tabel 3). Hal serupa terjadi pada tanaman nenas tangkit yang dikulturkan pada media MS dengan NAA 1 mg L -1 yang ditambahkan dengan BAP 2 atau 3 mg L -1 dapat menambah jumlah tunas, tetapi pemberian NAA di atas 3 mg L -1 menghambat pertambahan jumlah tunas meskipun dikombinasikan dengan BAP (Zulkarnain and Neliyati, 2017). Nazir et al, (2022) juga melaporkan pada tanaman obat Valeriana jatamansi bahwa rata-rata panjang tunas tertinggi diperoleh pada media MS dengan NAA 1 mg L -1 dan BAP 2 mg L -1 .…”

Section: Hasil Dan Pembahasanunclassified

Pengaruh BA dan NAA terhadap multiplikasi tunas ubi kayu secara in vitro

Yelli¹,

Ardian²,

Utomo³

2022

J. AGRO

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Ubi kayu varietas UJ-3, klon BW-1 dan Unila UK-1 banyak ditanam khususnya di Lampung. Untuk itu diperlukan bibit dalam jumlah besar yang dapat dilakukan secara in vitro. Penelitian ini bertujuan untuk mengevaluasi pengaruh BA dan NAA terhadap induksi dan multiplikasi tunas ubi kayu secara in vitro. Penelitian dilakukan menggunakan rancangan acak lengkap (RAL) dengan dua faktor, yaitu : (1) Varietas/klon yang terdiri atas UJ-3 (K1), BW-1 (K2) dan Unila UK-1 (K3), dan (2) Media yang terdiri atas media Murashige and Skoog (MS) tanpa zat pengatur tumbuh (M1), MS + BA 0,1 mg L-1 (M2), MS + BA 0,1 mg L-1 + NAA 0,05 mg L-1 (M3), MS + BA 0,3 mg L-1 + NAA 0,05 mg L-1 (M4), MS + BA 0,5 mg L-1 + NAA 0,05 mg L-1 (M5). Setiap perlakuan diulang sebanyak empat kali. Hasil menunjukkan bahwa tunas dari varietas/klon ubi kayu dapat terinduksi pada semua media perlakuan. Rata-rata jumlah tunas tertinggi (1,25 tunas) dihasilkan pada media M4 untuk klon Unila UK-1, jumlah buku paling tinggi (8 buku) dihasilkan klon BW-1 pada media M3 dan M4, jumlah daun hijau tertinggi (6 helai) pada media M3. Klon BW-1 juga menghasilkan jumlah daun gugur tertinggi (4,5 helai) pada media M4. ABSTRACTCassava UJ-3, BW-1, and Unila UK-1 are widely planted especially in Lampung. Therefore, it needs a high number of planting materials that can be done through tissue culture. This study aimed to determine the effect of BA and NAA on in vitro of cassava shoot induction and multiplication. The study used a completely randomized design with two factors, namely: (1) Variety/clone which consisted of UJ-3 (K1), BW-1 (K2), and Unila UK-1 (K3), and (2) Medium which consisted of MS without growth regulators (M1), MS + BA 0,1 mg L-1 (M2), MS + BA 0,1 mg L-1 + NAA 0,05 mg L-1 (M3), MS + BA 0,3 mg L-1 + NAA 0,05 mg L-1 (M4) and MS + BA 0,5 mg L-1 + NAA 0,05 mg L-1 (M5). Each treatment was repeated four times. Results showed that shoots from all genotypes could be induced in all treatment mediums. The highest shoot number (1.25 shoots) was produced by Unila UK-1 on M4 medium. The BW-1 clone showed the best result in the node number (8 nodes) on the M3 and M4 medium, the green leaves number (6 sheets) on the M3 medium, and produced a high deciduous leaves number (4.5 sheets) on the M4 medium.

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Pengaruh NAA dan BAP terhadap Kultur Jaringan Nenas Tangkit (Ananas comosus (L.) Merr. cv. Tangkit)

Cited by 9 publications

References 0 publications

Optimasi Induksi Tunas Aksiler Dendrobium nobile Melalui Kombinasi 2-iP dan 2,4-D In Vitro

Optimasi Induksi Tunas Aksiler Dendrobium nobile Melalui Kombinasi 2-iP dan 2,4-D In Vitro

Effect of tomato (Solanum lycopersicum) extract on height and number of roots in patchouli (Pogostemon cablin Benth.) subculture planlet

Pengaruh BA dan NAA terhadap multiplikasi tunas ubi kayu secara in vitro

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