PENGARUH VARIASI ZAT PENGATUR TUMBUH IAA, BAP, KINETIN TERHADAP METABOLIT SEKUNDER KALUS SIRIH HITAM  (Piper betle L. Var Nigra)

Junairiah, Junairiah; Amalia, Nurul; Manuhara, Yosephine Sri Wulan; Ni’matuzahroh, Ni’matuzzahroh; Sulistyorini, Lilis

doi:10.20473/jkr.v4i2.16898

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Likewise, better regeneration from the globular stage SE in Kenyan cassava was noted when decreased concentration or no PGRs were added in medium (Ngugi et al, 2015). It can be concluded that PGRs plays an important role in the level of callus induction prior to embryogenesis, especially the auxins that affect dedifferentiation and induces cell division (Junairiah et al, 2019). It was also noted that the growth of SEs was asynchronous in both UJ-3 and BW-1 genotypes, this is in corroboration with earlier reports on E. globules (Corredoira et al, 2015) and Leptadenia reticulata (Patel et al, 2021).…”

Section: Discussion Discussion Discussionsupporting

confidence: 86%

Somatic embryogenesis in two cassava (Manihot esculenta Crantz) genotypes

Yelli

TITIN²,

UTOMO³

et al. 2023

Not Bot Horti Agrobo

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Plant breeding through hybridization in cassava is facing a problem due to inconsistent flowering, and also the donor genes controlling superior traits are limited. An alternative method of breeding is through genetic transformation, and regeneration via somatic embryogenesis is promising route to achieve this. As somatic embryogenesis in cassava is genotype-specific, in the present study a protocol has been developed for UJ-3 and BW-1 genotypes. Immature sterile leaves from 7-10 days axillary shoots in a pre-condition medium were used as an explant. Leaves were inoculated on Murashige and Skoog (MS) medium containing picloram (0.0, 7.5, 10.0, 12.5, and 15.0 mg/L) and 1-naphthalene acetic acid (NAA, 6 mg/L) for induction of somatic embryos (SEs). Genotype BW-1 showed best results as early callus formation time i.e. 8.04±0.32 days after induction (dai) compared to UJ-3 (8.67±2.13 dai). The callus fresh weight (0.64 g) was also higher in BW-1 than UJ-3 (0.38 g) after 4 weeks in callus induction medium (CIM), and the callus formation ranges between 85.19±3.70 to 96.30±3.70% for both genotypes. Subculturing embryogenic callus to MS+CuSO4 (4 µM) + picloram (6 mg/L) +NAA (0.5 mg/L) (SK1 medium) germinated maximum SEs in BW-1 (46.56±36.86), whereas the number was less for UJ-3 (11.89±11.90). Further, shoots were developed from green cotyledons followed by hardening and acclimatization of plantlets.

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Section: Discussion Discussion Discussionsupporting

confidence: 86%

Somatic embryogenesis in two cassava (Manihot esculenta Crantz) genotypes

Yelli

TITIN²,

UTOMO³

et al. 2023

Not Bot Horti Agrobo

View full text Add to dashboard Cite

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“…Perlakuan K0I0 merupakan perlakuan kontrol yang tidak diberikan zat pengatur tumbuh Kinetin atau IAA, dengan tinggi eksplan rata-rata 1,1 cm. Hal ini mendukung pendapat (Junairiah et al, 2019), menunjukkan bahwa hormon yang disintesis oleh tanaman (endogen) dan hormon yang diberikan kepada tanaman (eksogen) mempunyai pengaruh yang signifikan terhadap proses pemanjangan sel pada tanaman. Gambar 2 menunjukkan dinamika pertumbuhan rata-rata tinggi plantlet.…”

Section: Tinggi Eksplan (Cm)unclassified

PENGARUH KOSENTRASI HORMON KINETIN DAN IAA PADA KULTUR JARINGAN TANAMAN MANGROVE (Rhizophora apiculate BL)

Mahadi,

Wulandari,

Kusmedi

2024

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Mangroves (Rhizophora apiculata) are one of the plants that grow in mangrove forests. The significant degradation of mangrove forests in Indonesia requires effective and efficient rehabilitation efforts. Efforts to provide superior seeds in a short time and in large quantities, one of which is through tissue culture. ZPT (Growth Regulator) Kinetin and IAA in balance can stimulate the morphogenesis process in the explant. This study used a 4×4 factorial randomized complete (RAL) design with 3 replications. The first factor is the concentration of kinetin. 0 mg/L, 1 mg/L, 3 mg/L, and 5 mg/L. The second factor is IAA concentrations of 0 mg/L, 0.5 mg/L, 1 mg/L, and 1.5 mg/L. The parameters observed were explant growth time, explant growth percentage, and bud height. The results showed an interaction with the plant growth time factor, and the best plant growth rate was achieved using the kinetin treatment of 5 mg/L and the IAA treatment of 1.5 mg/L, while the best shoot height factor was found in the kinetin treatment of 5 mg/L. l and IAA 1 mg/L treatment concentrate.

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PENGARUH VARIASI ZAT PENGATUR TUMBUH IAA, BAP, KINETIN TERHADAP METABOLIT SEKUNDER KALUS SIRIH HITAM (Piper betle L. Var Nigra)

Cited by 2 publications

References 14 publications

Somatic embryogenesis in two cassava (Manihot esculenta Crantz) genotypes

Somatic embryogenesis in two cassava (Manihot esculenta Crantz) genotypes

PENGARUH KOSENTRASI HORMON KINETIN DAN IAA PADA KULTUR JARINGAN TANAMAN MANGROVE (Rhizophora apiculate BL)

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