The inventory of fish species is required in developing a fisheries control plan, knowing the fish distribution and the potential of fishes as a basis for water spatial planning. This research aims to evaluate the fish diversity of freshwater fishes in Aceh Tamiang. An explorative study was conducted to investigate freshwater fishes in rivers, reservoirs, and other lotic and lentic ecosystems. Specimens were catched using fishing net, gill net and fish trap. The sampling locations were spread over 10 villages and data were collected from July to August 2023. The water quality parameters observed were temperature, pH, DO and brightness. This study reported a total of 26 species belonging to 11 families including: Cyprinidae, Osphronemidae, Cichlidae, Channidae, Synbranchidae, Clariidae, Zenachopteridae, Bagridae, Anabantidae, Nandidae, and Eleotridae. Whereas Cyprinidae and Osphronemidae are the most dominant families found. Morphometric of body weight varied from 0.6 to 156 g and total length 4.6 to 23.6 cm. The factor condition ranged of 0.26 to 7.95. This study showed the diversities of fishes in Aceh Tamiang which may be important for fisheries management. Furthermore, it serves as a basis for future efforts in domesticating freshwater fish in Aceh.