A toxicity test is a test carried out to estimate the degree of damage caused by a compound to biological or non-biological materials. One of the compounds known to have anticancer, antibacterial, and antifungal effects is Fe(II) N-benzyl methyl dithiocarbamate. This compound has cytotoxic activity on liver cells. The purpose of this study was to determine the LD50 value of Fe (II) N-benzyl methyl Dithiocarbamate and to determine the acute toxicity category of Fe (II) N-benzyl methyl Dithiocarbamate. From the results of research that have been carried out the synthesis of compounds capable of forming complex compounds of Fe (II) N-benzylmethyl dithiocarbamate which causes no death in male and female mice with an unknown LD50 value, categorized as non-toxic, meaning that this complex compound does not have a toxic effect on male mice. and female.