Pengzhenrongella phosphoraccumulans sp. nov., isolated from high Arctic glacial till, and emended description of the genus Pengzhenrongella

Abstract

A yellow pigmented, Gram-stain-positive, motile, facultatively anaerobic and irregular rod-shaped bacteria (strain M0-14T) was isolated from a till sample collected from the foreland of a high Arctic glacier near the settlement of Ny-Ålesund in the Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that M0-14T formed a lineage within the family Cellulomonadaceae, suborder Micrococcineae. M0-14T represented a novel member of the genus Pengzhenrongella and had highest 16S rRNA gene sequence similarity to Pengzhenrongella sicca LRZ-2T (97.3 %). Growth occurred at 4–25 °C (optimum 4–18 °C), at pH 6.0–9.0 (optimum pH 7.0), and in the presence of 0–5 % (w/v) NaCl. The predominant menaquinone was MK-9(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major polar lipids were phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol, one undefined phospholipid and five undefined phosphoglycolipids. The cell-wall diamino acid was l-ornithine whereas rhamnose and mannose were the cell-wall sugars. Polyphosphate particles were found inside the cells of M0-14T. Polyphosphate kinase and polyphosphate-dependent glucokinase genes were detected during genomic sequencing of M0-14. In addition, the complete pstSCAB gene cluster and phnCDE synthesis genes, which are important for the uptake and transport of phosphorus in cells, were annotated in the genomic data. According to the genomic data, M0-14T has a metabolic pathway related to phosphorus accumulation. The DNA G+C content of the genomic DNA was 70.8 %. On the basis of its phylogenetic relationship, phenotypic properties and chemotaxonomic distinctiveness, strain M0-14T represents a novel species of the genus Pengzhenrongella, for which the name Pengzhenrongella phosphoraccumulans sp. nov. is proposed. The type strain is M0-14T (= CCTCC AB 2012967T = NRRL B-59105T).

Pengzhenrongella frigida sp. nov., isolated from a glacier

“…In addition, P. phosphoraccumulans M0-14 T had phosphatidylglycerol, which was absent in strain HLT2-17 T . The sole menaquinone is MK-9 (H 4 ), which was consistent with the characteristics of Pengzhenrongella species [1, 3].…”

“…The main fatty acids (>5 %) of strain HLT2-17 T were anteiso-C 15 : 0 (36.6%), C 16 : 0 (15.4%), summed feature 3 (8.4 %), anteiso-C 15 : 1 A (6.8%), iso-C 14 : 0 (6.2%), iso-C 16 : 0 (5.3%). Strain HLT2-17 T and P. phosphoraccumulans M0-14 T [3] both contained anteiso-C 15 : 0 and C 16 : 0 as the major fatty acid. In contrast, P. sicca LRZ-2 T contained more anteiso-C 15 : 1 [1].…”

“…There is a significant difference in polar lipid composition between strain HLT2-17 T and its closest relative, P. phosphoraccumulans M0-14 T (). For example, strain HLT2-17 T contained DPG, which was absent in P. phosphoraccumulans M0-14 T [3]. In addition, P. phosphoraccumulans M0-14 T had phosphatidylglycerol, which was absent in strain HLT2-17 T .…”

“…At the time of writing, this genus comprises only two species with validly published and correct names [2]. The type strains of Pengzhenrongella sicca [1] and Pengzhenrongella phosphoraccumulans [3] was isolated from a soil sample collected from the High Arctic tundra [1]. During a comprehensive survey of bacterial diversity on the surfaces of glaciers in PR China, we isolated a significant number of bacterial strains.…”