Penicillin and Tetracycline as Contamination Control Agents in Alcoholic Fermentation of Sugar Cane Molasses1

Carbon dioxide released from alcoholic fermentation accounts for 33% of the whole CO(2) involved in the use of ethanol as fuel derived from glucose. As Arthrospira platensis can uptake this greenhouse gas, this study evaluates the use of the CO(2) released from alcoholic fermentation for the production of Arthrospira platensis. For this purpose, this cyanobacterium was cultivated in continuous process using urea as nitrogen source, either using CO(2) from alcoholic fermentation, without any treatment, or using pure CO(2) from cylinder. The experiments were carried out at 120 μmol photons m(-2) s(-1) in tubular photobioreactor at different dilution rates (0.2 ≤ D ≤ 0.8 d(-1) ). Using CO(2) from alcoholic fermentation, maximum steady-state cell concentration (2661 ± 71 mg L(-1) ) was achieved at D = 0.2 d(-1) , whereas higher dilution rate (0.6 d(-1) ) was needed to maximize cell productivity (839 mg L(-1) d(-1) ). This value was 10% lower than the one obtained with pure CO(2) , and there was no significant difference in the biomass protein content. With D = 0.8 d(-1) , it was possible to obtain 56% ± 1.5% and 50% ± 1.2% of protein in the dry biomass, using pure CO(2) and CO(2) from alcoholic fermentation, respectively. These results demonstrate that the use of such cost free CO(2) from alcoholic fermentation as carbon source, associated with low cost nitrogen source, may be a promising way to reduce costs of continuous cultivation of photosynthetic microorganisms, contributing at the same time to mitigate the greenhouse effect.

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“…Penicillin (500 u.i. mL −1 ) was added to prevent contamination 15. Continuous feeding was started after 12 h of batch fermentation.…”

Section: Methodsmentioning

confidence: 99%

CO₂ from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source

Matsudo

Bezerra

Converti

et al. 2011

Biotechnology Progress

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“…It is critical to prevent growth of the contaminating lactobacilli by following suitable guidelines for cleaning and sanitation, acid washing of yeast destined for re-use and the appropriate use of antibiotics during fermentation (Heist 2009). In the fuel ethanol industry, control of bacterial contamination is achieved by the use of antibiotics such as penicillin G, streptomycin, tetracycline (Day et al 1954;Aquarone 1960), virginiamycin (Hynes et al 1997;Islam et al 1999) and monensin (Stroppa et al 2000) alone or in combination. However, the concept of antibiotic use in industrial processes is controversial.…”

Section: Methods and Resultsmentioning

confidence: 99%

The role of nisin in fuel ethanol production with Saccharomyces cerevisiae

Peng

Zhang

et al. 2012

Letters in Applied Microbiology

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Aims: To investigate the effects of nisin on lactobacilli contamination of yeast during ethanol fermentation and to determine the appropriate concentration required to control the growth of selected lactobacilli in a YP/glucose media fermentation model. Methods and Results: The lowest concentration of nisin tested (5 IU ml−1) effectively controlled the contamination of YP/glucose media with 106 CFU ml−1 lactobacilli. Lactic acid yield decreased from 5·0 to 2·0 g l−1 and potential ethanol yield losses owing to the growth and metabolism of Lactobacillus plantarum and Lactobacillus brevis were reduced by 11 and 7·8%, respectively. Approximately, equal concentrations of lactic acid were produced by Lact. plantarum and Lact. brevis in the presence of 5 and 2 IU ml−1 nisin, respectively, thus demonstrating the relatively higher nisin sensitivity of Lact. brevis for the strains in this study. No differences were observed in the final ethanol concentrations produced by yeast in the absence of bacteria at any of the nisin concentrations tested. Conclusions: Metabolism of contaminating bacteria was reduced in the presence of 5 IU ml−1 nisin, resulting in reduced lactic acid production and increased ethanol production by the yeast. Significance and Impact of the Study: Bacteriocins represent an alternative to the use of antibiotics for the control of bacterial contamination in fuel ethanol plants and may be important in preventing the emergence of antibiotic‐resistant contaminating strains.

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“…The bioethanol industry often uses various antibiotics such as penicillin G, tetracycline, virginiamycin, or a cocktail of these antibiotics against Lactobacillus spp. and other bacterial species in fermentations at a range of concentrations from 0.1 mg/L to 20 mg/L [82][83][84][85]. Among the antibiotics, virginiamycin has been widely considered for yeast fermentation since it shows activity against lactobacilli at lower pH values than other antibiotics [86].…”

Section: Antibioticsmentioning

confidence: 99%

Anti-Contamination Strategies for Yeast Fermentations

Seo

Park

Jung³

et al. 2020

Microorganisms

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Yeasts are very useful microorganisms that are used in many industrial fermentation processes such as food and alcohol production. Microbial contamination of such processes is inevitable, since most of the fermentation substrates are not sterile. Contamination can cause a reduction of the final product concentration and render industrial yeast strains unable to be reused. Alternative approaches to controlling contamination, including the use of antibiotics, have been developed and proposed as solutions. However, more efficient and industry-friendly approaches are needed for use in industrial applications. This review covers: (i) general information about industrial uses of yeast fermentation, (ii) microbial contamination and its effects on yeast fermentation, and (iii) currently used and suggested approaches/strategies for controlling microbial contamination at the industrial and/or laboratory scale.

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Penicillin and Tetracycline as Contamination Control Agents in Alcoholic Fermentation of Sugar Cane Molasses1

Cited by 29 publications

References 6 publications

CO₂ from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source

CO₂ from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source

The role of nisin in fuel ethanol production with Saccharomyces cerevisiae

Anti-Contamination Strategies for Yeast Fermentations

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Penicillin and Tetracycline as Contamination Control Agents in Alcoholic Fermentation of Sugar Cane Molasses1

Cited by 29 publications

References 6 publications

CO2 from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source

CO2 from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source

The role of nisin in fuel ethanol production with Saccharomyces cerevisiae

Anti-Contamination Strategies for Yeast Fermentations

Contact Info

Product

Resources

About

CO₂ from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source

CO₂ from alcoholic fermentation for continuous cultivation of Arthrospira (Spirulina) platensis in tubular photobioreactor using urea as nitrogen source