Penicillin-Binding Protein 1A, 2B, and 2X Alterations in Canadian Isolates of Penicillin-Resistant
            <i>Streptococcus pneumoniae</i>

Nichol, Kim; Zhanel, George G.; Hoban, Daryl J.

doi:10.1128/aac.46.10.3261-3264.2002

Cited by 50 publications

(53 citation statements)

References 25 publications

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“…These findings suggest that antibiotic pressure is capable of promoting genetic rearrangement in S. pneumoniae. The alteration ratios for PBPs have been reported by a few investigators (9,21), but these pieces of information were restricted to the TP domains of PBPs. Clinical practitioners in Japan prefer to use cephalosporins instead of penicillins for the treatment of community-acquired infections, and the prevalence of cephalosporin-resistant S. pneumoniae strains in Japan has been increasing year by year (26).…”

Section: Vol 48 2004 Complete Sequences Of Six S Pneumoniae Pbp Gementioning

confidence: 99%

Complete Sequences of Six Penicillin-Binding Protein Genes from 40 Streptococcus pneumoniae Clinical Isolates Collected in Japan

Sanbongi

Ida

Ishikawa

et al. 2004

Antimicrob Agents Chemother

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All six penicillin-binding protein (PBP) genes, namely, pbp1a, pbp1b, pbp2a, pbp2b, pbp2x, and pbp3, of 40 Streptococcus pneumoniae clinical isolates, including penicillin-resistant S. pneumoniae isolates collected in Japan, were completely sequenced. The MICs of penicillin for these strains varied between 0.015 and 8 g/ml. In PBP 2X, the Thr550Ala mutation close to the KSG motif was observed in only 1 of 40 strains, whereas the Met339Phe mutation in the STMK motif was observed in six strains. These six strains were highly resistant (MICs м 2 g/ml) to cefotaxime. The MICs of cefotaxime for 27 strains bearing the Thr338Ala mutation tended to increase, but the His394Leu mutation next to the SSN motif did not exist in these strains. In PBP 2B, the Thr451Ala/Phe/Ser and Glu481Gly mutations close to the SSN motif were observed in 24 strains, which showed penicillin resistance and intermediate resistance, and the Thr624Gly mutation close to the KTG motif was observed in 2 strains for which the imipenem MIC (0.5 g/ml) was the highest imipenem MIC detected. In PBP 1A, the Thr371Ser/Ala mutation in the STMK motif was observed in all 13 strains for which the penicillin MICs were м1 g/ml. In PBP 2A, the Thr411Ala mutation in the STIK motif was observed in one strain for which with the cefotaxime MIC (8 g/ml) was the highest cefotaxime MIC detected. On the other hand, in PBPs 1B and 3, no mutations associated with resistance were observed. The results obtained here support the concept that alterations in PBPs 2B, 2X, and 1A are mainly involved in S. pneumoniae resistance to ␤-lactam antibiotics. Our findings also suggest that the Thr411Ala mutation in PBP 2A may be associated with ␤-lactam resistance.

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Section: Vol 48 2004 Complete Sequences Of Six S Pneumoniae Pbp Gementioning

confidence: 99%

Complete Sequences of Six Penicillin-Binding Protein Genes from 40 Streptococcus pneumoniae Clinical Isolates Collected in Japan

Sanbongi

Ida

Ishikawa

et al. 2004

Antimicrob Agents Chemother

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“…However, some pneumococcal strains did not belong to these classes and cannot be detected by class-specific PCR assays (24,25). Genetic analysis of pbp2b sequences in penicillin-resistant strains were reported from Japan, Spain, Iceland, Canada, France, the United Kingdom, Papua New Guinea, South Africa, and the United States (7,10,11,17,19,20,21,25). In Korea, the pbp2b gene sequences of multidrug-resistant S. pneumoniae isolates were reported (23).…”

mentioning

confidence: 99%

Unique Variations of pbp2b Sequences in Penicillin-Nonsusceptible Streptococcus pneumoniae Isolates from Korea

Baek¹,

Ko²,

et al. 2004

J Clin Microbiol

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pbp2b gene alterations were analyzed in 102 clinical isolates of Streptococcus pneumoniae (30 penicillin susceptible, 23 intermediate, and 49 resistant) from Korea. On the basis of PBP2B amino acid sequences, penicillin-nonsusceptible isolates of S. pneumoniae belonged to six groups, and 76% of the isolates in groups I to IV showed the same divergent block of amino acid alterations. Thirteen isolates (group II) also possessed a divergent block that was identical to that of Streptococcus oralis. The pbp2b genes of most Korean isolates showed novel mosaic mutations due to horizontal gene transfer. The Thr2523Ala substitution, previously thought to be associated only with penicillin-nonsusceptible strains, was also found in three penicillinsusceptible strains. On the basis of their pbp2b nucleotide sequences, all penicillin-nonsusceptible isolates can be detected by multiplex PCR, which can be used as a novel method for detection of antibiotic-resistant pneumococcal strains in clinical specimens.

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“…Approximately 1-kb fragments of pbp1a, pbp2x, and pbp2b encompassing the penicillin-binding domain of each gene were PCR amplified as described by Nichol et al (17) and sequenced by ACGT (Wheeling, IL). Competition assays were performed with a protocol based on the work of Sifaoui et al (20), using Bocillin-FL (Invitrogen, Carlsbad, CA).…”

mentioning

confidence: 99%

Activities of Ceftobiprole and Other β-Lactams against Streptococcus pneumoniae Clinical Isolates from the United States with Defined Substitutions in Penicillin-Binding Proteins PBP 1a, PBP 2b, and PBP 2x

Davies

Shang

Bush

2006

Antimicrob Agents Chemother

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The activities of ceftobiprole and other ␤-lactams were examined with 30 Streptococcus pneumoniae isolates containing multiple pbp1a, pbp2b, and pbp2x mutations. The highest ceftobiprole MIC was 1 g/ml, while the comparator MICs were 16 to 64 g/ml. Fifty percent inhibitory concentrations for penicillin-binding protein 2x were 0.5 g/ml (ceftobiprole) and 4 g/ml (ceftriaxone) in a penicillin-and ceftriaxone-resistant isolate.

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Penicillin-Binding Protein 1A, 2B, and 2X Alterations in Canadian Isolates of Penicillin-Resistant Streptococcus pneumoniae

Cited by 50 publications

References 25 publications

Complete Sequences of Six Penicillin-Binding Protein Genes from 40 Streptococcus pneumoniae Clinical Isolates Collected in Japan

Complete Sequences of Six Penicillin-Binding Protein Genes from 40 Streptococcus pneumoniae Clinical Isolates Collected in Japan

Unique Variations of pbp2b Sequences in Penicillin-Nonsusceptible Streptococcus pneumoniae Isolates from Korea

Activities of Ceftobiprole and Other β-Lactams against Streptococcus pneumoniae Clinical Isolates from the United States with Defined Substitutions in Penicillin-Binding Proteins PBP 1a, PBP 2b, and PBP 2x

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