Penicillin resistance in soil bacteria is an index of soil metal content near a porphyry copper deposit and near a concealed massive sulfide deposit

Watterson, John R.; Nagy, L.; Updegraff, David M.

doi:10.3133/ofr8416

Cited by 8 publications

(4 citation statements)

References 24 publications

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“…Antibiotic producers can evolve mechanisms to protect themselves from the compounds they are producing (Hopwood, 2007). A study in the US Geological Survey has unraveled very strong correlations between heavy metal concentrations in soil and the resistance of Bacillus isolates to penicillin (Watterson et al, 1984). Extreme resistance was observed, with up to 5 mg ml À1 of penicillin, and the isolates belonging to the B. cereus clade were among the most resistant (Watterson et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

“…A study in the US Geological Survey has unraveled very strong correlations between heavy metal concentrations in soil and the resistance of Bacillus isolates to penicillin (Watterson et al, 1984). Extreme resistance was observed, with up to 5 mg ml À1 of penicillin, and the isolates belonging to the B. cereus clade were among the most resistant (Watterson et al, 1984). Bacillus VU-DES13 was isolated from F. candida, that is producing this class of secondary metabolites (Suring et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Genome annotation and antimicrobial properties of Bacillus toyonensis VU‐DES13, isolated from the Folsomia candida gut

Agamennone¹,

Straalen

Brouwer³

et al. 2019

Entomologia Exp Applicata

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Antibiotic resistance necessitates the search for new bioactive compounds with novel mechanisms of action. Natural products derived from bacteria and fungi are widely used in the field of medicine and new environments can be explored as sources of antimicrobials. Bacteria associated with springtails have shown high inhibitory activity against pathogens. Here, we characterized a bacterial strain with high potential for antimicrobial activity, isolated from the gut of the springtail Folsomia candida Willem (Collembola: Isotomidae). The strain was characterized using the ‘analytical profile index’ and the ‘minimal inhibitory concentration’ assay to test for antibiotic resistance. Agar overlay and agar disk diffusion assays were used to test the inhibitory activity of the strain and its extract against a variety of pathogens, and reporter assays were used to investigate the mode of action. High‐performance liquid chromatography was used to analyze and fractionate the extract of bacterial culture, followed by additional assays on the fractions. The genome of the strain was screened for presence of antibiotic resistance genes and secondary metabolite gene clusters. The isolate was identified as Bacillus toyonensis Jiménez et al., but it displayed differences in metabolic profile when compared to the type species. The isolate was highly resistant to penicillin and inhibited the growth of a variety of pathogenic microorganisms. Genome analysis revealed an enrichment of resistance genes for β‐lactam antibiotics compared to the type isolate. Also, secondary metabolite clusters involved in the production of siderophores, bacteriocins, and nonribosomal peptide synthetases were identified. In conclusion, a unique Bacillus strain was isolated from the gut of F. candida, for which we provide evidence of inhibitory activity against an array of pathogens. This, coupled with high resistance to penicillin as substantiated by the presence of resistance genes, points to the potential of B. toyonensis VU‐DES13 to provide a new source of antimicrobial compounds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome annotation and antimicrobial properties of Bacillus toyonensis VU‐DES13, isolated from the Folsomia candida gut

Agamennone¹,

Straalen

Brouwer³

et al. 2019

Entomologia Exp Applicata

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“…This pattern suggests selection for a species that is chromosomally antibiotic resistant rather than selection for plasmids carrying metal and antibiotic resistance. Watterson et al (59) found that high levels of penicillin resistance in Bacillus spp. from metalliferous soil resulted from selection of Bacillus cereus, which has chromosomally encoded 1Blactamase.…”

Section: Discussionmentioning

confidence: 99%

“…from metalliferous soil resulted from selection of Bacillus cereus, which has chromosomally encoded 1Blactamase. This selection apparently results from an abundance in these soils of penicillin-producing molds (58), which Watterson et al (59) suggest may be due to the metal-binding ability of penicillamine, a main product of penicillin hydrolysis. Further study is clearly required to understand the basis for metal resistance and the significance of antibiotic resistance in these and other environmental isolates.…”

Section: Discussionmentioning

confidence: 99%

Alteration of a Salt Marsh Bacterial Community by Fertilization with Sewage Sludge

Hamlett

1986

Appl Environ Microbiol

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The effects of long-term fertilization with sewage sludge on the aerobic, chemoheterotrophic portion of a salt marsh bacterial community were examined. The study site in the Great Sippewissett Marsh, Cape Cod, Mass., consisted of experimental plots that were treated with different amounts of commercial sewage sludge fertilizer or with urea and phosphate. The number of CFUs, percentage of mercury-and cadmium-resistant bacteria, and percentage of antibiotic-resistant bacteria were all increased in the sludge-fertilized plots. Preliminary taxonomic characterization showed that sludge fertilization markedly altered the taxonomic distribution and reduced diversity within both the total heterotrophic and the mercury-resistant communities. In control plots, the total heterotrophic community was fairly evenly distributed among taxa and the mercury-resistant community was dominated by Pseudomonas spp. In sludge-fertilized plots, both the total and mercury-resistant communities were dominated by a single Cytophaga sp.

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Geomicrobiology applied to mineral exploration in Mexico

Melchior¹,

Cardenas²,

Dejonghe³

1994

Journal of Geochemical Exploration

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Penicillin resistance in soil bacteria is an index of soil metal content near a porphyry copper deposit and near a concealed massive sulfide deposit

Cited by 8 publications

References 24 publications

Genome annotation and antimicrobial properties of Bacillus toyonensis VU‐DES13, isolated from the Folsomia candida gut

Genome annotation and antimicrobial properties of Bacillus toyonensis VU‐DES13, isolated from the Folsomia candida gut

Alteration of a Salt Marsh Bacterial Community by Fertilization with Sewage Sludge

Geomicrobiology applied to mineral exploration in Mexico

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